Pathogen Control Program

Question 1

Define refined and traditional quarantine methods.

Answer 1

Refined - Imports into the main facility only progeny from previously imported surface disinfected embryos that were reared to adults and spawned in the quarantine facility.
Traditional - Imports surface-disinfected embryos from adults housed and spawned within the quarantine facility.

Question 2

How are chorionated embryos disinfected?

Answer 2

20-50 ppm bleach, buffered to pH 7.0

Question 3

What is an example of a pathogen with vertical transmission that can circumvent quarantine procedures?

Answer 3

P. neurophilia

Question 4

A sentinel surveillance program should use fish exposed to what type of water?

Answer 4

Pre- and post-filtration. Pre-filtration fish associated with dirty sumps, post-filtration fish on the same rack as colony fish.

Question 5

How long should sentinel fish be placed for? Why?

Answer 5

At least 3 months to detect chronic infections like mycobacteria or P. neurophilia