Environmental Factors Flashcards

1
Q

Define poikilotherm

A

Cold-blooded, rely on external sources for temperature regulation.

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2
Q

As a general rule, a change in temperature should be limited to what? Can fish tolerate larger shifts? How do ZF compare?

A

+/- 1.5C/day, although many aquatic organisms can tolerate later shifts in temp well after an initial shock and brief period of acclimation. Zebrafish among most eurythermal, with an acclimated thermal tolerance of 6.7-41.7C.

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3
Q

How does temperature impact metabolism and oxygen demand?

A

Increased temp speeds up metabolism and increases oxygen demand. Metabolic rate doubles for each 10C increase in temperature. ZF larvae less tolerant.

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4
Q

What is the outcome of maintaining life support systems at slightly lower temperatures?

A

Increase available oxygen, reduce need for food, and minimize losses due to accidental temp increases. Lower temps decrease rate of growth and development.

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5
Q

How does temperature impact reproductive/hormonal induced activity?

A

Temp change (usually an increase) is a factor in initiation of repro activity or other hormonal induced activity.

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6
Q

At what temperature should the ambient room be maintained? Why? Room temperatures should not be maintained above what temperature? Why?

A

At least 1-2 degrees above that of the containment system water to prevent condensation of water on the external surface of the aquaria, walls, and floors, which can serve as a medium for mold or fungi and negatively impact air quality. Do not exceed room temperatures of 80F (27C) due to cost, lower dissolved oxygen saturation, higher metabolic rate of fish, and lab worker discomfort.

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7
Q

Describe the oxygen content at the air-water interface.

A

Slightly higher, thus why fish gasp or sip at the water surface with low oxygen concentration.

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8
Q

What can result in supersaturation of water?

A

Massive aeration, rapid heating of cold water, cavitating water pumps, air stones placed too close to the water pump inflow lines

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9
Q

In general, sufficient aeration can be provided by what? Oxygen diffusion is adequate to what depth?

A

Maintaining appropriate circulation. Oxygen diffusion adequate up to 4 inches.

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10
Q

As a general rule for recirculating aquaculture systems, how often should the tank water be turned over?

A

One complete turnover per hour.

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11
Q

Compressed air may be added to the recirculating system, usually at what point?

A

Within the filtration zone.

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12
Q

What is a disadvantage of providing compressed air to water?

A

Is often hot and dry, which accelerates evaporation. Air bubbling into aquaria can produce a fine mist that acts as a fomite and aids in transmission of pathogens. Mists can also corrode metal components, resulting in scale that can build up and drop into tanks resulting in increased levels of toxic metals.

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13
Q

How can the introduction of scale or rust from metallic components of an aquaculture system be prevented?

A

Regularly evaluate components. Seal with an appropriate protectant or paint. Replace components or scrape routinely.

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14
Q

What pH range do ZF prefer? What are negative outcomes of higher and lower pH?

A

7-8. pH > 8 = Higher concentrations of toxic unionized ammonia. pH <5 = Inhibits activity of nitrifying bacteria, increasing total ammonia levels due to accumulation

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15
Q

In closed recirculating systems, how does the pH normally trend?

A

Gradually decrease due to production of acids during nitrification process as ammonia is converted to nitrate. Also decreases in poorly aerated systems due to production and accumulation of CO2 by respiring fish.

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16
Q

How does pH influence the susceptibility of fish to toxins?

A

Common metallic components are more soluble in water under acidic conditions, so toxicity associated with exposure is more common in systems maintained at a lower pH.

17
Q

Define conductivity. What does it directly measure?

A

Indicator of the total amount of dissolved ions in a solution, including sodium and other ionized minerals. Direct measure of electric current and an aqueous solution can conduct.

18
Q

How are changes in salinity monitored?

A

Direct measurements of salinity are difficult, so conductivity is a convenient method to imprecisely measure the salinity.

19
Q

At what salinity range can ZF be maintained at? Larvae?

A

125 to 2500 microseimens. Larvae reared at salinities up to 5g/l from day 5-10 postfertilization.

20
Q

Define water hardness. How is it usually measured via commercial test kits and water quality reports?

A

Amount of calcium and magnesium salts in the water. Other cations also contribute, but are usually present in very small quantities.
Commercial kits measure calcium carbonate. Water quality reports measure in ppm or mg/l.

21
Q

What hardness of water do ZF prefer? What is the result of very soft water (0-10 ppm)?

A

Hard water species, preferring levels between 80-200 ppm. Very soft water is detrimental to developing fry as they rely on water for mineral uptake during early growing phases of life. Low water hardness or Ca associated with low embryo survival rates and increased susceptibility to other environmentally induced disease.

22
Q

What must occur when utilizing reverse osmosis or distilled water for ZF?

A

Add minerals back. Can add calcium carbonate, crushed coral, or aragonite preparations.

23
Q

What are ammonia-oxidizing bacterial species? What other organisms play a role?

A

Nitrosomonas, Nitrospira, and Nitrosococcus. Archae also play a role.

24
Q

What species oxidize nitrite?

A

Primary Nitrospira, also Nitrobacter.

25
Q

What is the role of bacterial species of fish flora in the nitrogen cycle?

A

Contribute to conversion of nitrogenous wastes.

26
Q

At what level is nitrate toxic to fish? Why is it important to maintain lower levels of nitrate?

A

At levels approaching or exceeding 200 mg/l. Maintain lower levels to ensure health of biofilter and control algal growth.