Path Flashcards
What should samples be fixed in?
at least 10x their volume of bufferred formalin.
What stain is used for fat?
Oil Red O
What stain is used for fibrous tissue?
massons trichome - stains ct green
What stain is used for haemosiderin?
Perls Prussian blue
What is pyknosis?
Small shrunken densely staining nuclei
What is karyorrhexis?
The nuceus has fragmented into small pieces
What is karyolysis?
Nucleus has lysed
What is lipidosis?
Accumulation of intracytoplasmic lipid. the central role of the liver means that it is especially susceptible. the major mechanisms are excessive FFas into the liver or toxic damage affecting metabolism of fatty acids and triglycerides.
What is pseudomelanosis?
green/black discolouration due to conversion of iron to iron sulphide by GI bacteria.
What is biliary imbibition?
Pigment imbibed - onto liver and any organs in contact e.g GI tract.
How does Rigor mortis occur?
Beings approx 2-4 hours after death. Burst of metabolic activity as substrates depleted on cessation of circulation progressive decrease in muscle Ph, Oxygen and ATP. Ca2+ efflux from sarcoplasmic reticulum of muscle cell > contraction of muscle fibres. Generally the head and neck musculature is affected first then the changes spread to the extremities. disappears as putrefaction begins (approx 1-2 days) Completely disappeared by 72 hours.
what PM signs are seen when an animal is euthanased with barbiturates?
Spleen is congested & crystal deposition on the endocardium of the heart.
What is splenic siderofibrosis?
yellow, dry encrustations on the capsule of the spleen thought to represent sites of previous local haemorrhage with usbsequent deposits of Fe, Ca and fibrosis.
What does steroid use do to the liver?
long term steroid use can lead to steroid induced hepatopathy. pathogenesis : glucocorticoids induce the enzyme glycogen synthetase leading to increased storage of glycogen within hepatocytes. The hepatocytes in midzonal areas are often preferentially affected and can be up to 10x normal size.
What is the role of the renin-angiotensin and aldosterone system?
Main role is in maintaining effective circulating volume. Renin is produced by specialised juxtaglomerular cells in the glomerular afferent arertiole. renin is released in response to decreased renal perfusion due to decreased blood pressure and decreased blood volume. Renin converts angiotensin to angiotensin I which then converts to angiotensin II. Angiotensin II increases aldosterone production by adrenal cortex > aldosterone enhances renal sodium absorption.
What is the function of ADH?
ADH is made in the hypothalamus and released when hypothalamic osmoreceptors are stimulated. ADH controls permeability of distal tubules and collecting ducts. if ADH is present, urine volume is low. if ADH is absent, urine volume is high. Another stimulus for ADH secretion is decreased blood volume and pressure. Baroreceptors in the left atrium are stimulated by increased blood volume. Impulses pass in neurons to the hypothalamus where they inhibit ADH producing cells > urine volume is high which reduces blood volume and pressure. Decreased BP leads to decreased firing of baroreceptors and increased ADH secretion > urine volume is low > increased blood volume and pressure.
What are Atrial natriuretic peptides?
A group of diverse peptide hormones produced by the heart in response to stretching of the heart, they reduce cardiac output and blood pressure through loss of NA and water from the kidneys as well as through vasodilation.
What are the possible reasons for hypokalaemia?
Increased insulin activity, anorexia in herbivores, low K diet, GI disease especially horses, profuse sweating in horses, increased renal loss.
What are the possible reasons for Hyperkalaemia?
Diabetes mellitus, high K fluid therapy, post renal obstruction, anuric renal failure, addisons disease.
What are the possible reasons for Hypomagnesaemia?
Hypoporteinaemia, decreased absorption (grass tetany), anorexia, excess urinary excretion eg e.g ketonuria, blister beetle poisoning in horses, lactation tetany in shetland pony mares.
What are the possible reasons for hypermagnesaemia?
Decreased renal excretion (renal failure, Decreased GFR), increased PTH (milk fever), increased intestinal absorption. Excess Iv administration.
What are the possible reasons for hypocalcaemia?
Milk fever and eclapmsia in bitches, hypoproteinaemia, chronic renal failure, acute pancreatitis.
What are the possible reasons for hypercalcaemia?
Malignant neoplasia
Renal failure in hrose
Thiazide diuretics, increased vit D activity.
What is the protein content of transudate and exudate comparitively?
Tranusdate is 0.05-0.5% mainly albumin. Exudate is usually 2-4%, but can be higher. Transudate does not coagulate as no fibrinogen but exudate contains fibrinogen so will coagulate.