Part3 Flashcards
blank is distinct for bees from same colony - can tell nest mates from robbers
skin oils
bee skin called
cuticle
how can pathogens get into bees
food, contact with flowers, nest mates, fecal matter
what 2 things in gut offer protection against virus/bacteria
1- symbiotic bacterial communities in gut
2 - acidic pH of gut
what is innate immunity
immunity consistently present across life of organism
humans have what kind of immunity
adaptive (remember past attackers by keeping markers in blood)
6 types of social immunity
1 - grooming
2 - undertaking
3 - requeening
4 - absconding
5 - siting the nest
6 - propolizing the nest
what is it called when bees groom each other
allogrooming - remove objects/pathogens from nest mates
how old are undertaker bees
2-3 weeks old
what might colonies under stress from parasites/pathogens do with queen
replace her
when would bees abscond x 4
mite levels too high
hive damage
repetitive bear attacks
environmental problems
what is propolis
wax mixed with plant resin
presence of propolis reduces what and does what for colony
reduces expression of immune related genes, which is good means colony is healthier/less stressed with propolis
after what type of infection will colony increase # of resin foragers
chalkbrood
honeybee microbiome is
community of beneficial microorganisms found in gut
where do bees acquire gut bacteria
from hive environment and nestmates - touching fecal matter (at age 4-6 days old)
3 parts of IPM
1 - support honey bee immunity
2 - track the pest or parasite population
3 - targeted intervention by beekeeper
how does a beekeeper support honey bee immunity x6
provide access to flowers
supplement nutrition
clean and dry space
protection from heat/cold
strong genetic stock
cultural controls
4 ways to track pest/parasite population for IPM
1 - whether pests are affecting colony
2 - how those levels change over time
3 - whether or not high enough levels to affect colony - require intervention
4 - was intervention effective
wax moths in strong colony will
build slowly/keep to corners
wax moths in weak colonies will
destroy brood combs w/in weeks to months - not usually direct cause of colony death
wax worms raised commercially for
fish bait/feeding reptiles, birds, other small mammals
what other new study about wax moths is cool
they can eat plastic bags, decomposing polyethylene plastic films into ethylene glycol
2 species of wax moths
lesser and greater
wax moth eggs laid where
cracks/crevices or unprotected comb
how long until wax moths hatch
3 to 8 days in warm weather, up to 1 month when cold
what do wax worms eat
nectar, honey, pollen, bee bread, wax, cocoons of molting larvae/pupae in brood comb
how long can wax worm feeding phase last
5 months
how long until adult moths emerge and waht do they do
a week to 2 months - leave hive to harden wings and mate, come back to lay eggs
how long do wax moths live
avg of 12 days
what size/what do wax worms look like
between 1/2 inch to 3/4 inch, grey/brown in color with long thin antennae
difference between wax worm and small hive beetle larvae
twice as big as small hive beetle and SHB have no prolegs, wax moths have 4
how do shb vs wax moth larvae feel differet
shb rubbery
wax moth squish
indications of wax moths x 3
webbing on surface of frames (bees can’t remove)
fecal matter on webbing
bald brood could be - will uncap own larvae if wax moths beneath
when do wax moths usually fly
at night
wax moth eggs - how many are laid at once and what do they look like
laid in masses 50-100 but too small to be visible
wax moth larvae - looks like x 4
3/4 inch long - white with dark head but darken as they age
3 pairs of legs
multiple body segments
small prolegs
wax moth pupae - found where and look like
touch white cocoons are 1/2 to 3/4 inch long and usually stuck in oval depressions
wax moth adults
between 1/2 to 3/4 inch long with wingspan 1and 1/4 to 1/ and 1/2 inch
less wax moths are smaller
how to prevent wax moths x 5
strong hive - will remove larvae and prevent adults from entering
only supering when bees bring in nectar
freeze equipment or heat to 115 for 1.5 hours
store in airtight containers/bags
expose boxes to light
are there wax moth treatments for active hive
no
2 chemical treatments for wax moths
1 - honey supers fumigated with co2 - doesn’t affect honey
2- hive bodies but not honey can be fumigated with PDB crystals
If using PDB is it affective against eggs
no - needs to be used continously
how long before reusing a hive treated with PDB
at least 48 hours aired out
SHB arrived in US what year
1998 in FL
what can an intense SHB infection do
ferment honey - makes slimy discolored and frothy
SHB is a problem when
population small/colony weak or have too much space to defend
how many eggs can SHB lay
1000-2000
how fast do shb eggs hatch
in 3-5 days
how long do SHB larvae feed on bee resources
10-14 days or more
what do shb do after feeding
leave hive at night and got 4-8 inches down in soil to pupate
how long are shb pupating in soil
3-4 weeks
what do SHB do after pupating
fly back into hive at dusk - can identify hives by pheromones
how long after returning to hive do shb mate
1 week
how long is entire lifecycle of shb
4-6 weeks
how long can adult shb live
up to 6 months
what does shb do in winter
stops reproducing and keeps warm/alive with bee cluster
what does shb do to honey when their population is high
defecate and gets slimy/greasy - smells like rotting oranges -humans & bees will reject
shb eggs look like and are where
similar in appearance to bee eggs but smaller
laid in clusters
small puncture in cap of brood cell visible
shb larvae looks like
whiteish with brown heads much larger than adult beetles
resembles wax moths but wax moth larger
have 3 pairs of legs next to head but no additional prolegs like wax moths
shb pupae are where/look like
underground - not apparent
pearly white and darken over 3-4 weeks of pupating
adult shb are size/color
1/4 inch long and .13 wide
dark brown turn black when mature
6 legs and club like antennae
adults prefer top of hives
3 ways to prevent shb
keep apiaries/honey houses free of combs, cappings, wet frames
promptly replace uneaten pollen patties and extra supers
controlled with beetle traps/swiffers - pesticides in ground last resort
how far can adult shb fly
8 miles
how long can adult shb survive without food
10 days
are there protocols to measure level of shb infestation
no
what humidity level to keep honey house at to delay shb from hatching
below 50% humidity
2 synthetic chemicals to treat shb
checkmite +
gardstar
should you use checkmite+ and why
no - ingredients stay in frames for many years - fertility problems in queens
should you use gardstar and why
no - main ingredient permethrin is toxic to bees and animals
4 ways to reduce wasps as pests
1 - entrance reducers/robber screens
2 - no cracks/holes in hives - wasps can smell the honey
3 - don’t use open feeders - dripping honey while working, keeping colony open for extended time
4 - wasp traps at edges of apiary
wasps eat blank and blank
honey and dead adults & brood
what is nosema
disease of digestive system - fungal gut parasite cause by 2 different species
what are the two species of nosema
apis and ceranae
can a colony have both types of nosema
yes
is dysentery a symptom of nosema ceranae
no
which version of nosema is still prevalent today
ceranae - apis almost wiped out
which version of nosema is specific to honeybees
apis, ceranae can affect other bees too - bumbles
how does a bee get infected with nosema and how does it spread
spores enter body
spread through fecal->oral route or through grooming or sexually transmitted
how many infected spores in midgut one infected bee
30-50 million
symptoms of nosema in individual bees x5
1 = bee has less energy - parasite uses energy
2 - starved state and eat more food - less able to make royal jelly
3 - start foraging earlier - not 100% on why (helps spread disease to others?)
4 - less likely to return home - disoriented
5 - die sooner - avg 9 dies earlier
can nosema affect more than workers
yes - all castes
colony level symptoms of nosema
reduced honey production
decreased adult population
sometimes death - not common in USA
reduced brood production
if a colony dies from nosema where do the adult bees die
far away from hive
dysentery is usually a sign of what
nutrition issues
fermented honey, dark honey, unripened honey or sugar with high moisture content
how to tell if your colony has nosema - 2 ways
send sample to beltville lab in maryland - free
at least 100 bees collected alive
get back spore count per bee
OR count yourself under microscope
name of device used to count blood cells
hemocytometer
when is nosema most common during year
spring but high levels in fall can kill over winter
nosema infections can exacerbated by
pesticides
are there registered treatments for nosema that are effective
no
ways to prevent/manage nosema x5
good drainage & sun during winter
good ventilation
spring - supplement with syrup & pollen if jelly levels are low
replace old comb with foundation to prevent accumulation of spores
combs that are from colonies with nosema should be disinfected before used again
how to disinfect combs with nosema - 4 ways
1 - freeze combs/hive bodies at least 4 days
2 - fumigate combs/hives with acetic acid - lay pads on top bars of hive body for 1 week
3 - expose combs/hives to heat - at least 120 for 24 hours or 140 for 15 mins
4 - expose to gamma radiation
tips to control nosema infection
move to isolated apiary if higher than 1 million spores per bee
support with syrup and pollen
increase population
treat if all else fails
2 natural products to help nosema infections
1 - nosevit - oak bark extract
2 - hivealive - seaweed extracts, thymol, lemongrass oil
is fumagillin an effective treatment for nosema
no - was used to treat apis may make cerane worse
what is fumagillin
antibiotic derived from funus aspergillus fumigatus
how does fumagillin work
inhibits an enzyme in nosema parasite, METAP2
only targets parasite and doesn’t kill spores
remaining spores can reinfect bees
side effects of fumagillian on bees if given regularly
impact digestive health - low levels alter midgut proteins
METAP2 enzyme is in all organisms not just nosema
side effects of fumagillian on humans
abdominal cramps
weight loss
can you apply fumagillian when honey supers present
no
what is the leading cause of colony losses
varroa mites
how many and names of varroa species
2 varroa jacobsoni and varroa destructor
which varroa species is bigger and more ovular
destructor
which type affects western honey bees
only destructor - jacobsoni can’t reproduce
which type of bee originally had varroa
asian honey bee - moved to others in 1960s to western bee
when and where did varroa enter US
1987 - florida - hives transported to wisconsin from fl
when did every contigous state have varroa
1990s (hawaii 2007)
where are the areas without varroa
newfoundland canada and australia (had a case it was destroyed)
where do varroa mites reproduce
brood cells (prefer drone)
2 stages in varroa life cycle
1 - reproductive - mite inside cell reproducing and feeding on pupae
2 - phoretic - mite on outside of adult bee feeding on bee
when do varroa enter brood cells - worker vs drone
worker = 20 hours before capped
drone = 40 hours before capped
how many more time likely varroa to be in drone cell vs worker cell
9x
how many days after cell is capped does varroa lay egg and what sex of egg
3 days and male
how many eggs and how often does varroa lay egg after first egg - how many total per cell
every 30 hours or so - female
total 1-6 female eggs
what color are varroa when hatching male vs female
male - white to light pink and small
female - white but darken with age
how many days for varroa to reach sexual maturity - both sexes
6 days
when the bee leaves the cell after being with varroa what happens to the varroa
mated females go out on the bee, the male and immature females die in the cell
on avg how many mites released per cell worker vs drone
1.3 worker
2.6 drone
how long are mites on adult bees feeding
13 days - 7 avg
what type bees do varroa prefer and why
nurse bees - closer to brood plus better nutrition
in apis cerana (asian honey bee) varroa can only reproduce where
in drone brood
what do varroa mites transmit to bees and what can it become if advanced
viruses and called parasitic mite syndrome
when does varroa peak in northeast
late summer - fall, need to monitor aug - oct
when making winter bees
4 ways varroa impact health
1 - feed on fat bodies = shortened lifespan
2 - transmit viruses through wounds in bee cuticle - can sicken/kill bees
3 - can suppress bee immune system
4 - can kill entire colonies if unmanaged = die within 6months - 2 years
problem if bees parasitized in fall
may not develop into winter bees - varroa reduces lipid levels
problem if bees parasitized during development
smaller hypophargengeal glands and lower protein levels as adults (ability to feed larva)
varroa is a vector for how many bee viruses
at least 8
Parasitic mite syndrome is when and 3 things happening in colony with it
the most advanced stage of varroa infestation happens in a colony
diseased brood with 1 or more viruses
adults with deformed wings
visible mites on adults
signs of hygenic behavior with varroa
spotty brood
perforated cappings
chewed down brood
how fast after getting PMS stage can colony collapse
within 1-4 weeks
how much does varroa grow it’s population per month
doubles once per month
IPM for varroa
keep levels low all year
manage mites before hitting dangerous levels
develop scheduling plan to regularly monitor
what percentage of colonies should you monitor for varroa
10%
what level of mites to take action - both spring and fall
if 2 per 100 Apr-July
if 3 per 100 Aug-October
what kind of bees do you want to sample with alcohol wash/sugar shake
nurse bees on brood
what percentage alcohol to use in alcohol wash
70% of better
methods for measuring varroa that don’t work x4
visual observation
uncapping drone brood
sticky board
ether roll - car starter fluid
today what is considered a severe infection of mites - #, %
over 3000
2-3%
2-3 per 100
3 stages of hygenic behavior
chew holes in cappings
bald brood
chewed down brood