Parasitology Flashcards
What is viewed on 4x
Tapeworm segments, fleas, ticks, mites, and lice
What is viewed on 10x
Scan and locate eggs, cysts, and larvae
What is viewed on 40x
ID eggs, Direct scan for cysts, larvae, and Giardia
Direct Smear procedure
- put 1-2 drops of saline on a slide 2. Mix a fleck of stool sample onto slide, add cover slip 3. with condenser down- scan on 10x and ID on 40x
Scan and ID Girdia on ____x
40x
Fecal Float procedure
- Fill fecalyzer 2/3 full with Fecasol or Zinc Sulfate 2. Add pea size fecal sample, mix well 3. Let sit 8-10 min with cover slip on top 4. Put cover slip on slide and scan on 10x, ID on 40x
Fasciola Hepatica
Liver Fluke
Paragonimus Kellicotti
Lung Fluke
Dipylidium Caninum
Flea Tapeworm Hexacanth ova in egg basket
Taenia Taeinaformis
Cat-Rat Tapeworm Radial striations, hexacanth ova
Anoplocephala spp.
Tapeworm Equine IM host- Orbatid mite
Moniezia spp.
Tapeworm Sheep and Cattle IM host Orbatid mite
Toxocara spp.
Roundworm Can be passed transplacentally Pot-belly appearance in puppies and kittens
Toxoscaris Leonina
Roundworm Arrowhead worm Cats and dogs
Ascaris suum
Roundworm pigs
Parascaris equorum
equine roundworm
Ancylostoma caninum
Hookworm
Braziliense
Hookworm Cat and Dog
Tubaeformae
Hookworm Cat
Uncinaria Stenocephala
Cold-weather hookworm Dogs
HOCT Complex
Haemonchus Ostertagia Cooperia Trichostrongylus
Heamonchus
Ruminants Barber pole worm
Ostertagia
Sheep and Cattle Brown Stomach worm
Cooperia
Cattle, Sheep, Goats Bankrupt worm
Trichostrongylus
Sheep and Cattle Black Scour worm
Trichuris vulpis
Whipworm Dogs Double operculated ova “Stewie”
Dirofilaria immitis
Heartworm ELISA test detects adult female antigens Vector- mosquito
Demodex
Burrowing Mite (mange) Deep skin scraping
Sarcoptes scabei
Burrowing mite seen in dogs
Notoedres cati
Burrowing mite seen in cats
Cheyletiella spp.
Walking dandruff Palpal claws Light skin scraping
Otodectes
Ear mite Waste looks like coffee grounds Roll sample on slide
Ctenocephalides
Flea Scotch tape prep
Giardia
Protozoa Trophozoites and Cysts
Coccidia
Protozoa Isospora- Sm animal (ID) Eimeria- Lg animal
What are 2 types of general parasite tests?
Qualitative- is there a parasite or not (more common) Quantitative- How many does the animal have (more for research)
3 samples for quantitative testing
Fecal Urine Blood
3 qualitative tests
Gross exam Direct smear/cytology Fecal float
What do you wright if not ova or parasites are seen on a fecal?
NSATT
What can be ID’d on a Fecal Float?
Parasite ova, Coccidia, and Giardia
How long are fecal samples spun at and using what type of centrifuge?
1200-1500 rpm for 3 min. Swinging head
Fecal cytology/smear procedure
- Roll a fleck of fecal sample on slide in a thin layer 2. allow to dry/heat fix 3. Stain with Dif-Quik 4. Examine on 100x oil immersion
What is ID’d on a fecal cytology
Clostridium- safety pins Campylobacter- Seagulls
Dif-Quik staining procedure
Jar 1- Fixative- 5, 1sec dips Jar 2- Eosinophilic- 5, 1sec dips Jar 3- Basophilic- 7-10, 1sec dips Rinse with water and allow to dry
Strongylus vulgaris
Large strongyle equine bloodworm
Oxyuris equi
Pinworm
Rhipicephalus sanguineus
Brown Dog Tick
Cutebra
Rabbit bot
Parasite transmittable to pregnant women vis the litter box
Toxoplasmosis gondi
Parasite causing VLM
Roundworms
Parasite causing CLM
Hookworms
Dioctophyma renale
Giant Kidney worm
Pearsonema capillaria
urine sample Bladderworm
What is a Facultative parasite?
Only the female is pathogenic
Hookworms
Blood feeders Reported as hookworms in sm animal and TASE in LA.