Microbiology

Question 1

Gram stain procedure

Answer 1

1. Bacteria on slide in drop of distilled water
2. Air dry and heat fix
3. Crystal Violet- 1 min
4. Gram’s Iodine- 1 min
5. Decolorizer- until clear
6. Safranin- 1 min

Question 2

Gram Positive Bacteria

Answer 2

Purple

Question 3

GPCOC

Answer 3

Staphylococcus and Streptococcus

Question 4

Catalase Test

Answer 4

H2O2
Bubbles- Staph
No Bubbles- Strep

Question 5

Coagulase Test

Answer 5

0.5mls Rabbit Plasma
No clot- non pathogenic Staph
Clot- pathogenic Staph

Question 6

Small GPROD

Answer 6

Corynebacterium, Listeria, Erysipelothrix, Arcanobacterium, and Rhodococcus.

Question 7

Large GPROD

Answer 7

Bacillus and Clostridium (safety pins)

Question 8

Branching Rods

Answer 8

Nocardia, Actinomyces, Dermatophilus, and Streptomyces

Question 9

Gram Negative Bacteria

Answer 9

Pink

Question 10

Oxidase Test

Answer 10

Filter Paper
No change- enteric
Purple/dark color on filter paper- non enteric

Question 11

Enteric GNROD

Answer 11

E. Coli, Klebsiella, Proteus, Salmonella, Shigella, and Yersinia.

Question 12

Non-Enteric GNROD

Answer 12

Brucella, Pasturella, Franciscella, Pseudomonas, Bordetella, Moraxella, and Campylobacter (sea gulls)

Question 13

Spirochete

Answer 13

Leptospira and Borrelia burgdorferi (Lyme Dz)

Question 14

Lyme Dz

Answer 14

Vector- Ixodes tick

Reservoir- white footed mouse

Question 15

Molds

Answer 15

Microsporum canis, Aspergillus, and Trichophyton

Question 16

Microsporum canis

Answer 16

Ringworm
Mold
Blue canoes
Woods lamp
Stain with Lactophenyl Cotton Blue

Question 17

Yeast

Answer 17

Malassezia, Candida, and Cryptococcus neoformans.

Question 18

Malassezia

Answer 18

Peanut Men
Otitis in dogs
Roll ear swab on slide, heat fix, and stain with Wrights

Question 19

Cryptococcus neoformans

Answer 19

Meningitis
Budding yeast
India Ink Prep

Question 20

Dimorphic Fungus

Answer 20

Mold in soil then become yeast in tissues.

Blastomyces, Histoplasma, Coccidioides, and Sporothrix.

Question 21

Mycobacterium

Answer 21

Tuberculosis

Question 22

Clamydophila psittaci

Answer 22

Psittacosis

Question 23

Rickettsia rickettsii

Answer 23

RMSF

Question 24

Ehrlichia canis

Answer 24

Tropical canine pancytopenia

Question 25

Bartonella

Answer 25

Cat scratch Dz

Question 26

Listeria monocytogenes

Answer 26

Circling Dz

Question 27

Streptococcus equi

Answer 27

Strangles

Question 28

Yersinia pestis

Answer 28

Plague

Question 29

Fusobacterium spp.

Answer 29

Thrush

Question 30

Beta Hemolysis

Answer 30

Complete lysis of RBC’s in blood agar

Question 31

Alpha Hemolysis

Answer 31

Incomplete lysis, brown/green zone around colony

Question 32

Gamma Hemolysis

Answer 32

No lysis

Question 33

Kirby-Bauer Susceptibility Test

Answer 33

Tests certain bacteria’s resistance to certain types of abx. Clearing measured in mm.
Resistant- no clearing
Intermediate- grey area
Susceptible- clearing around disk

Question 34

MacConkeys Agar

Answer 34

Selects for GNROD, lactose and non-lactose fermenters.
Bright Pink- lactose fermenter
Colorless- non lactose fermenter

Question 35

Isolation Streaking

Answer 35

… Know how to do it!

Question 36

What does MRSA stand for?

Answer 36

Methicillin Resistant Staph Aureus

Question 37

The Tetanus immunization is a ____? Who is most susceptible?

Answer 37

Toxoid

Humans and horses

Question 38

3 examples of micro-organisms

Answer 38

Bacteria, Fungi, Virus

Question 39

The Coggins test is used to dx what?

Answer 39

Equine Infectious Anemia (EIA)

Question 40

What is the vector that transmits the plague? Reservoir host?

Answer 40

Vector- flea

Reservoir- prairie dogs/cats

Question 41

Who do cows typically get rabies from?

Answer 41

Bats Bats Bats… And some times skunks

Question 42

Brucella abortis

Answer 42

Bangs Dz
GNROD
Vax young females (4-5mo)
Tattoo & orange tag in ear