Hematology Flashcards

1
Q

16g Needle

A

Grey Pigs

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2
Q

18g Needle

A

Green Horse, Cow, Large breed dogs

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3
Q

20g Needle

A

Pink Dogs

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4
Q

22g Needle

A

Blue Small dogs, cats

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5
Q

25g needle

A

Red Avian, neonates, Cats (femoral vein)

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6
Q

Purple Top Tube (PTT or LTT)

A

EDTA CBC

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7
Q

Green Top Tube (GTT)

A

Heparin Blood Gases

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8
Q

Blue Top Tube (BTT)

A

Sodium Citrate Coagulation Studies Tube must be full, mix, spin, plasma goes in another tube, refrigerate

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9
Q

Grey Top Tube (GreyTT)

A

Oxylate Glucose Studies

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10
Q

Red Top Tube (RTT)

A

Nothing Blood Chemistries

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11
Q

Red Ring Crit

A

Heparin PCV/TP direct from pt

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12
Q

Blue Ring Crit

A

Nothing PCV/TP from PTT

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13
Q

Black Ring Crit

A

Heparin Avian/Reptile CBC

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14
Q

PCV

A

Packed Cell Volume % of whole blood that is made up of RBC’s

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15
Q

TP

A

Total Protein Amount of protein present in the fluid portion of blood

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16
Q

Plasma

A

the fluid portion of anticoagulated blood

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17
Q

Serum

A

the fluid portion of coagulated blood

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18
Q

RBC normal ranges

A

RBC/ul Dog 5-10 Cat 5-11

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19
Q

WBC normal ranges

A

WBC/ul Dog 6,000- 17,000 Cat 5,500- 19,500

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20
Q

Corrected WBC

A

Only do if >5 nRBC’s WBC x 100 / nRBC’s + 100

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21
Q

Platelet normal ranges

A

Dog/Cat 200,000- 500,000 plts/ul

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22
Q

MCV normal ranges

A

Dog 60-70 fl Cat 39-55 fl

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23
Q

MCHC normal ranges

A

Dog/Cat 30-36 g/dl

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24
Q

MCV math/units

A

PCV x 10 / RBC = fl

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25
Q

MCHC math/units

A

Hgb x 100 / PCV= g/dl

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26
Q

MCH math/units

A

Hgb x 10 / RBC= pg

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27
Q

Observed Retics math/units

A

Retics / 1000 x 100= %

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28
Q

Corr Obs Retics math/units

A

PCV x ObsRetics / PCV avg= %

29
Q

Abs Retics math/units

A

Retics / 1000 x RBC (whole #) = retic/ul

30
Q

Abs Rectic ranges

A

> 60,000= regenative anemia < 60,000= non-regenative anemia

31
Q

PCV normal ranges

A

Dog 37-55% Cat 30-45%

32
Q

TP normal ranges

A

Dog 5.0-7.0 g/dl Cat 5.0-8.0 g/dl

33
Q

5 differences between avian blood vs. Mammalian blood

A
  1. Mature nRBC’s (avian) v. Concave shape 2. Polychromasia common (avian) v. not common 3. Thrombocytes (avian) v. Platelets 4. Heterophils (avian) v. Neutrophils 5. Basophil is mononuclear (avian) v. Basophil is segmented
34
Q

Avian blood parasites

A

Hemoproteus spp. Leukocytoon spp.

35
Q

Toxic Neutrophils

A

Diffused cytoplasmic basophilia Dohle bodies Cytoplasmic vacuolization Toxic Azurophilic granules

36
Q

Granulocytes

A

Neutrophils (segs) Eosinophils Basophils

37
Q

Agranulocytes

A

Lymphocytes Monocytes

38
Q

2 types of Retics

A

Punctate- polka dots, more mature Aggregate- Clumped ribosomes, less mature, only count in cats and birds

39
Q

Why do we do a Retic count

A

to determine the bone marrow’s response to anemia

40
Q

How many RBC’s are counted for a retic count

A

1000 RBC and tally retics as you go

41
Q

How many WBC’s are counted for a diff

A

100 cells on 40x

42
Q

Normal BV rages

A

Dog 88 mls/kg Cat 66 mls/kg

43
Q

Normal BV math/units

A

Animals wight in kg x species norm= mls

44
Q

Fluid replacement math/units

A

wt in kg x % dehydrated x 1000= mls needed

45
Q

Fibrinogen math/units

A

TP - hp x 1000= mg/dl

46
Q

TP:F math/units

A

TP / F in g/dl= (no units)

47
Q

How do you spin a crit tube

A

6000 rpm for 5 min

48
Q

What is done on 10x in the feathered edge

A

WBC distribution, Plt clumping, Abnormal cells, Microfilaria

49
Q

What is done on 10x in the monolayer

A

WBC distribution Adequate size

50
Q

What is done on 40x in the monolayer

A

WBC diff Begin morphology

51
Q

What is done on 100x oil in the monolayer

A

RBC/WBC Morphology - size, shape, color, +/- inclusions. Always quantify as mild, mod, marked Plt assessment - 8-30 app adequate - < 8 app decreasd - > 30 app increased

52
Q

What is done on 10x in the body

A

RBC associations Rouleaux Agglutination

53
Q

How do you tell the difference between Rouleaux and Agglutination

A

Saline wash -Rouleaux will wash away, while Agglutination will stay.

54
Q

Abnormally shaped RBC’s

A

Poikiocytosis

55
Q

RBC’s with no central pallor

A

Spherocytes

56
Q

2 terms for nRBC’s depending on stain

A

Polychromatophil- Dif-Quik/Wright’s Reticylocyte- NMB

57
Q

RBC’s with long, irregular projections

A

Acanthocyte

58
Q

RBC fragments

A

Schistocytes

59
Q

RBC’s with small, spike-like projections

A

Echinocytes

60
Q

RBC’s with blister or vacuoles

A

Keratocytes

61
Q

RBC’s with more surface area then contents

A

Leptocytes

62
Q

RBC that has a pale colored mouth area, seen only in dogs with chondrodystrophy

A

Stomatocytes

63
Q

Punched out cell

A

Torocytes

64
Q

Blister cell seen in conjunction with Heinz bodies

A

Eccentrocyte

65
Q

Empty RBC

A

ghost cell

66
Q

RNA appearing as very small spots that stain blue

A

Basophilic Stippling

67
Q

Single or double inclusions on a RBC

A

Howell-Jolly Bodies

68
Q

Clear nipple like protrusion form a cell, caused by zinc toxicity or stress in cats

A

Heinz Bodies

69
Q

What is the anatomy of a crit tube

A

RBC’s, Buffy coat, Plasma Buffy coat= WBC’s and Plts