Paper 2 Knowledge Gaps: Content Flashcards
What is the test for reducing sugars?
Benedicts test
What is the method for testing reducing sugars?
Add Benedict’s reagent to the food and boil in a water bath
What change takes place to indicate the presence of reducing sugars in its test?
Goes from blue to a brick red precipitate
What is the test for starch?
Iodine test
Describe change that occurs to indicate the presence of starch
Goes from orange to blue black
Name the test for amino acids/ proteins
Biuret
Describe change that occurs to indicate the presence of protein
Goes from blue to lilac after adding the biuret reagent
Name the test for fats
Ethanol emulsion test
Describe the test for fats
Add ethanol to the food to dissolve the fat then add water.
Describe the colour change that occurs to indicate the presence of fat
Colourless to white emulsion
What is the route that the blood takes to become purified?
- blood is transported to the kidney through the renal artery
- blood is filtered at a high pressure and the kidney selectively reabsorbs any useful materials, eg: glucose, mineral ions and water. Any excess is sent to the ureter for excretion and eventually the bladder
- the purified blood eventually returns to circulation via the renal vein
What is the role of the kidney?
To regulate the levels of salt, ions and urea in the blood
What is urea and when is it produced?
- produced in the liver when excess amino acids are broken down
- main waste product removed in urine
- as it is not reabsorbed by the kidney
What occurs during kidney failure?
When both kidneys stop working, causing a build-up of waste substances
How does dialysis work?
- unfiltered blood is taken from a blood vessel in the arm, mixed with blood thinners / an anti-coagulant to prevent clotting and pumped through the dialysis machine
- inside the machine, blood and the dialysis fluid are separated by a partially permeable membrane
- the blood flows in the opposite direction as compared to the dialysis fluid, allowing for the creation of a concentration gradient for the exchange of substances occur
What are the stages the kidney works in?
- filtration
- selective reabsorption
- formation of urine
Describe what happens in filtration
- blood passes into the kidney’s many capillaries
- blood under high pressure at the start of the nephron, which helps the ultrafiltration of the blood
- small molecules are squeezed out and pass into the nephron tubule called the Bowman’s Capsule
–> Small molecules: urea, water, ions, and glucose - large molecules too big to fit through the wall and will remain in the blood
Where does filtration happen?
Glomerulus
Where does selective reabsorption happen?
proximal convuluted tubule
Describe selective reabsorption
- kidneys must reabsorb the useful molecules that just got filtered out
- allows those that are not needed to pass out and form urine
- reabsorption of water and ions occur in the loop of Henle and collecting areas of the tubule
Describe what happens during the formation of urine
- molecules that aren’t reabsorbed continue along the nephron tubule as urine
- this eventually passes down the bladder
- urine exits in the ureter
What molecules are reabsorbed by the kidney during reabsorption?
- all of the glucose initially filtered out
- as much water as the body requires to maintain a constant water level in the blood plasma
- as many ions as the body needs to maintain a constant balance of mineral ions in the blood plasma
What are the adaptations of a nephron?
- large surface area of contact between nephron and capillaries
- cell membrane of first convoluted tube has folds (microvilli) increasing the surface area
- cells that use active transport have lots of mitochondria
- cells of bowman’s capsule and glomerulus capillaries have spaces making them leaky
What does ADH do if the water potential is too high?
- less ADH is released from the pituitary gland
- making the collecting duct less permeable to water
- leads to more water being released in urination
What does ADH do if the water potential is too low?
- more ADH is released from the pituitary gland
- making the collecting duct more permeable to water
- less water remains in the tubule + more entering blood surrounding the tubule
- less water is released in urination
Explain the effect of the negative feedback system when the thyroxine levels in the blood are low
- stimulate the hypothalamus to release TRH
- which causes the release TSH by the pituitary gland
- this hormone triggers the release of thyroxine by the thyroid
- causing the blood levels to return to normal
Explain the effect of negative feedback when the thyroxine levels in the blood are high
- hypothalamus inhibits the production of TRH
- stopping production of TSH
- stopping the thyroxine from being released
- so blood levels can return to normal