Pancreatic Enzymes Flashcards
Amylase is also known as
Alpha-1-4-glucan-4-glucohydrolase
Catalyzes the breakdown of polysaccharides like starch and glycogen via alpha-1-6 branching linkages
Amylase
Major tissue source of Amylase
Pancreas, Salivary gland
Minor tissue source of Amylase
Adipose tissue, small intestine, skeletal muscles and fallopian tube
Increase amylase is seen on
Acute pancreatitis, renal failure, and parotitis
The fastest to elevate (2-12 hours rise after the onset of attack acute pancreatitis)
Amylase
Level of amylase in the circulation normalizes after
3-5 days
T/F: Amylase is NOT specific to acute pancreatitis since there are other source in other organs or glands (parotid/salivary gland)
TRUE
T/F: Increase levels of Amylase in the plasma, increase in the urine
TRUE
Smallest enzyme in the plasma, therefore, normally filtered by the renal glomerulus (present in urine)
Amylase
Amylase in urine is decreased since it is reabsorbed so amylase in plasma is increased
Renal failure
Salivary amylase
Ptyalin (fast moving) *s-type
Pancreatic amylase
Amylopsin (slow moving) *p-type higher level than s-type
Measures the disappearance of starch substrate
Starch-iodine complex (Dark Blue) → Decrease in color intensity
Amyloclastic
Alternative substrate for starch
Glycogen
Iodine + Glycogen will result in what color?
Mahogany Brown
Measures the appearance of the product
Saccharogenic
Measures the increasing color from the production of a product -chromogenic dye fragment
Chromogenic
Coupling or several enzyme systems to monitor amylase activity
Continuous Monitoring
Amylase activity is directly proportional to absorbance in what methods?
Chromogenic and Saccharogenic
Amylase activity is inversely proportional to absorbance in what method?
Amyloclastic
Lipase is also known as
Triaceylglycerol acylhydrolase
● Hydrolyzes the ester linkages of fats to produce alcohols and fatty acids
● Used as a digestive enzyme
Lipase
Hydrolysis of dietary glycerides in the intestine to 2-monoglyceride and fatty acids
Lipase
Early and the most specific marker for acute pancreatitis
Lipase
Is secreted by the pancreas and not affected by renal disorder and salivary gland dse
Lipase
Major tissue source of Lipase
Pancreas
Lipase is a large molecule that remains in the circulation for how many days?
7
Elevation of pancreatitis starts after
6 hours
Substrate used fro Cherry Crandall and Tietz method?
50% olive oil (triolein)
Titrating agent used for Cherry Crandall and Tietz
0.4 NaOH
Indicator in Cherry Crandall
Phenolpthalein
Indicator in Tietz
Thymolpthalein + veronal
Endpoint for Cherry Crandall and Tietz
Fatty acid (Oleic acid)
End color for Cherry Crandall
Pink
End color for Tietz
Blue
More pure form of triglyceride and is also used as a substrate for lipase test
Triolein
It is the reference method for test of Lipase
Cherry Crandall
Hemolyzed sample is not allowed because it will inhibit hemoglobin and will cause false _
Decrease
T/F: Higher the lipase activity, higher level of liberated triglyceride
TRUE
Estimation of liberated fatty acids
Turbidimetric methods
Triglyceride is hydrophobic/insoluble, more turbid sample is equal to
More light blocking
The higher the lipase activity, the __ the absorbance
Lower absorbance (Inversely proportional)
