Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MTAP 2 - CC2 (Trans-Enzymology) > Liver Enzymes > Flashcards

Liver Enzymes Flashcards

1
Q

Other name for Aspartate Aminotransferase

A

Serum glutamic-oxaloacetic transaminase (SGOT) or serum glutamate-oxaloacetate transaminase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Transfer of an amino group between aspartate and alpha- keto acids where oxaloacetate and glutamate are the products

A

AST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Involved in the synthesis and degradation of amino acids

A

AST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

AST is not liver-specific because it is widely distributed, highest activities in

A

Cardiac, Liver and Skeletal Muscles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Predominant isoenzyme in the serum

A

Cytoplasmic AST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Elevated when there is severe injury or damage to the cell (mitochondrial membrane is damaged allowing mitochondrial AST to elevate)

A

Mitochondrial AST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

After MI, AST levels begin to rise in __ hours,
peak (highest serum level) at __ hours and return
to normal in __ days

A

6 to 8 hours
24 hours
5 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

T/F; AST is released to a greater degree in chronic
disorder of the liver with progressive damage to
the hepatocytes.

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Uses malate dehydrogenase and monitors decrease in absorbance at 340 nm

A

Karmen Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Reduce, high, increased absorbance

A

NADH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Oxidized, decreased absorbance

A

NAD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the color of the product in Karmen Method?

A

Colorless

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Secondary/coupling/indicator enzyme, found in reagent in Karmen Method

A

Malate Dehydrogenase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The activity of AST is __ to the product of secondary enzyme (MD)

A

Directly proportional

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Alanine Aminotransferase (ALT) is also known as

A

Serum glutamic-pyruvic transaminase (SGPT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q
  • Transfer of an amino group between alanine and alpha-ketoglutarate where the product is pyruvate and glutamate
  • Increased in hepatocellular disorders
A

ALT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

More liver-specific than AST

A

ALT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Is a significant marker in the evaluation of the hepatic disorders, specifically acute inflammatory conditions

A

ALT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

ALT is used to:

A
  1. Monitor the course of hepatitis treatment
  2. Monitor the effects of drug therapy especially among px with chronic disease
  3. Also used to screen blood donors
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

More sensitive and specific for post-transfusion hepatitis and occupational toxic exposure

A

ALT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Secondary enzyme for ALT measurement

A

Lactate Dehydrogenase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

The AST/ALT Ratio
Differentiates the cause of hepatic disord

A

De Ritis Ratio

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Non-viral origin; AST is higher than ALT

A

Ratio >1

24
Q

Viral in origin; ALT is higher than AST

A

Ratio <1

25
Q

coenzyme for aminotransferases. This will serve as a prosthetic group

A

Pyridoxal Phosphate (Vitamin B6)

26
Q

Major organ affected SGOT/AST

A

Heart, muscle, liver

27
Q

Substrate of SGOT/AST

A

Aspartic Alpha Ketoglutaric acid

28
Q

End products of AST

A

Glutamic acid + Oxaloacetic acid

29
Q

Color developer used in AST

A

2,4 DNPH

30
Q

Color intensifier for AST

A

0.4 N NaOH

31
Q

Methods used for AST

A

Reitman and Frankel

32
Q

Major affected organ for SGPT/ALT

A

Liver

33
Q

Substrate for SGPT/ALT

A

Alanine alpha Ketoglutaric acid

34
Q

End product of SGPT/ALT

A

Glutamic acid + Pyruvic acid

35
Q

Color developer for ALT

A

2,4 DNPH

36
Q

Color intensifier methods

A

0.4 N NaOH

37
Q

Methods for ALT

A

Reitman and Frankel

38
Q

NDPH means

A

Dinitrophenylhyradizine

39
Q

is a colorimetric method for transaminase measurement

A

Reitman and Frankel

39
Q

Toxic Hepatitis

A

Both (AST and ALT)

40
Q

Acute Myocardial Infarction

A

AST

41
Q

Wolff-Parkinson White Syndrome (involuntary contraction of muscle)

A

AST

42
Q

Trichinosis (Parasitic infection of T. spiralis

A

AST

43
Q

Chronic Alcoholism

A

Both (AST and ALT)

44
Q

Dermatomyositis (muscle)

A

AST

45
Q

Hepatic Cancer

A

Both

46
Q

Reye’s syndrome (Liver)

A

Both

47
Q

Viral Hepatitis

A

Both

48
Q

Muscular Dystrophy

A

AST

49
Q

Acute pancreatitis

A

AST

50
Q

Catalyze the transfer of the y-glutamyl residue to y-glutamyl peptides to amino acids, H2O, etc.

A

Gamma-Glutamyltransferase (GGT)

51
Q

Uses for diagnosis hepatobiliary disorders and chronic alcoholism

A

GGT

52
Q

Most common donor of gamma-glutamyl peptide is

A

Glutathione

53
Q

A sensitive marker for the determination of ethanol intoxication

A

GGT

54
Q

Used as marker for occult alcoholism (hidden consumption)

A

GGT

55
Q

Assay for GGT

A

Szaz Assay

56
Q

The absorbance of __ in Szaz assay is measured at

A

p-nitroaniline
405-420

MTAP 2 - CC2 (Trans-Enzymology) (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions