Page 2 Flashcards

1
Q

Legal’s Test steps

A

3 cc urine + NaOH or KOH -> alkaline urine

Few drops Acetic acid (concentrated)

Few drops Na nitroprusside, red to purple if (+)

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2
Q

Legal’s test detects what ketone body exactly?

A

Acetone
Acetoacetic acid

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3
Q

Rothera’s Test steps

A

5 cc urine (fresh) + 1 gm of NH4 SO3

2 drops Na nitroprusside

NaOH overlay, yields purple color when (+)

Negative when no purple color after 10-15 minutes.

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4
Q

Rothera’s Test is negative, result?

A

No rxn after 10-15 minutes

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5
Q

Acetest steps

A

Put Acetest reagent tablet on surface

Few drops urine on tablet, turns purple after 30s if (+), compare to color chart

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6
Q

Which ketone tests uses a color chart?

A

Reagent Strip Test
Acetest

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7
Q

Manner of Reporting acetone for acetest

A

+1 (Trace)
: 25-50 mg%
+2 (Moderate)
: 200-250 mg%
+3 (Strong)
: 400-1000 mg%

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8
Q

Manner of Reporting diacetic acid for acetest

A

+1 (Trace)
: 10-20 mg%
+2 (Moderate)
: 25-40 mg%
+3 (Strong)
: >50 mg%

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9
Q

Why KOH or NaOH is added in Legal’’s Test

A

To produce an alkaline urine

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