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Question 1

Legal’s Test steps

Answer 1

3 cc urine + NaOH or KOH -> alkaline urine

Few drops Acetic acid (concentrated)

Few drops Na nitroprusside, red to purple if (+)

Question 2

Legal’s test detects what ketone body exactly?

Answer 2

Acetone
Acetoacetic acid

Question 3

Rothera’s Test steps

Answer 3

5 cc urine (fresh) + 1 gm of NH4 SO3

2 drops Na nitroprusside

NaOH overlay, yields purple color when (+)

Negative when no purple color after 10-15 minutes.

Question 4

Rothera’s Test is negative, result?

Answer 4

No rxn after 10-15 minutes

Question 5

Acetest steps

Answer 5

Put Acetest reagent tablet on surface

Few drops urine on tablet, turns purple after 30s if (+), compare to color chart

Question 6

Which ketone tests uses a color chart?

Answer 6

Reagent Strip Test
Acetest

Question 7

Manner of Reporting acetone for acetest

Answer 7

+1 (Trace)
: 25-50 mg%
+2 (Moderate)
: 200-250 mg%
+3 (Strong)
: 400-1000 mg%

Question 8

Manner of Reporting diacetic acid for acetest

Answer 8

+1 (Trace)
: 10-20 mg%
+2 (Moderate)
: 25-40 mg%
+3 (Strong)
: >50 mg%

Question 9

Why KOH or NaOH is added in Legal’’s Test

Answer 9

To produce an alkaline urine