Page 1

Question 1

The intermediate products of fat metabolism.

Answer 1

Ketone Bodies

Question 2

Give me 3 Ketone bodies.

Answer 2

Acetone, acetoacetic acid and ß-hydroxybutyric acid

Question 3

Why ketones do not appear on urine?

Answer 3

Fats undergo complete metabolism into CO2 + H2O, thus no ketone products.

Question 4

When do ketone bodies appear?

Answer 4

Fat metabolism increase.

Question 5

Ketone bodies conditions: (4)

Answer 5

Inability to metabolize carbs (DM)
Increased carb loss (Vomiting)
Inadequate carb intake (Weight loss, Starve)

Question 6

Reaction for ketone bodies?

Answer 6

Nitroprusside Reaction

Question 7

Nitroprusside reaction reactants + products?

Answer 7

Na nitroprusside + Acetoacetic acid -> Purple color

Question 8

Nitroprusside rxn limitation

Answer 8

Does not measure ß-hydroxybutyric acid, only acetone

Question 9

Nitroprusside reaction is used in these tests. What tests exactly?

Answer 9

Reagent Strip
Acetest

Legal’s Test
Rothera’s Test

Gerhardt’s Test
Hart’s Test

Question 10

Gerhardt’s Test principle

Answer 10

FeCl3 + Acetoacetic acid -> red color

Question 11

Hart’s Test principle

Answer 11

Acetone & acetoacetic acid removed via boiling, then:

ß-hydroxybutyric acid + hydrogen peroxide -> ???