pack 4 Flashcards
1
Q
Why is absolute ethanol necessary during the DNA binding step?
A
Absolute ethanol promotes DNA precipitation and binding to the silica membrane.
2
Q
What does the pre-wash solution remove from the sample?
A
The pre-wash solution removes residual salts and contaminants.
3
Q
What is the purpose of the wash solution?
A
To clean the DNA-bound silica by removing remaining impurities.
4
Q
How does the elution buffer facilitate DNA retrieval from the silica membrane?
A
The elution buffer rehydrates the DNA, releasing it from the silica surface.
5
Q
Why is it important to determine the concentration and purity of extracted DNA before using it in experiments?
A
Ensuring proper concentration and purity prevents reaction failures and ensures reliable experimental results.
