pack 2

Question 1

Name the three common methods of DNA extraction.

Answer 1

Phenol-chloroform, silica-based, and magnetic bead methods.

Question 1

What are the advantages of silica-based extraction over other methods?

Answer 1

Silica-based methods are faster, avoid toxic chemicals, and yield high-quality DNA.

Question 2

Why is it important to ensure no food or drink is consumed 30 minutes before buccal swab collection?

Answer 2

To prevent contamination of the DNA sample with food particles or foreign DNA.

Question 3

What is the purpose of adding proteinase K and lysis buffer during the lysis step?

Answer 3

Proteinase K breaks down proteins, while lysis buffer disrupts cell membranes and denatures proteins, releasing DNA.

Question 4

How does ethanol enhance DNA binding to the silica membrane?

Answer 4

Ethanol removes water, creating conditions that favor DNA binding to silica.