pack 3 Flashcards
1
Q
Why is it important to incubate the sample at 56°C during lysis?
A
Incubation at 56°C activates proteinase K and ensures efficient cell lysis.
2
Q
During the washing step, why are two different buffers used?
A
To sequentially remove proteins, salts, and other impurities.
3
Q
What is the role of the elution buffer in the final step of DNA extraction?
A
The elution buffer releases bound DNA from the silica membrane for collection.
4
Q
What is the role of proteinase K in DNA extraction?
A
Proteinase K digests proteins, including histones, freeing DNA from protein complexes.
5
Q
How do chaotropic salts in the lysis buffer aid in DNA isolation?
A
Chaotropic salts disrupt hydrogen bonds and solubilize cellular components, aiding DNA release.
