P53

Question 1

What are some P53 therapeutics?

Answer 1

1. Gendicine
2. Onyx adenovirus (oncorine)
3. Nutlins

Question 2

What does gendicine do and what cells do they attack? what is the before treatment or after treatment?

Answer 2

P53 negative cells

they replace the P53 gene by using an adenovirus to deliever P53

before treatment: tumor has +P53 and -P53

after treatment: all tumor cells have +P53

Question 3

what does onyx adenovirus (oncorine) and what cells do they target? what is the before treatment or after treatment?

Answer 3

they target P53 negative cells

it is a lytic virus (lysing and spreading through tumor cells)

only replicating in/infecting cells that are lacking P53 function

before treatment: tumor has +P53 and -P53

after treatment: tumor has only +P53 and the cells were lysed

Question 4

what does nutlins and what cells do they target? what is the before treatment or after treatment?

Answer 4

they target +P53 cells

small molecule protien modifier
need to have P53 produced in cell for this therapeutic to be effective
sequesters MDM2 to increase P53 activity

before treatment: tumor has +P53 and -P53

after treatment: P53 is activated

Question 5

What does HPV and cervical cancer do? what happens with E7 and E6?

Answer 5

E6: binds to p53 and inhibits its function
E7: binds to the RB protein and prevents the interaction of E2F

Question 6

Fill the blank: ___ of cancer have mutated P53

Answer 6

50%

Question 7

how does P53 regulate MDM2 protein?

Answer 7

MDM2 binds to N-terminal P53 transactivation domain; resulting in

no phosphorylation/activation of P53 and translocation of P53 into cytoplasm