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OMICS > P. aeroginosa Biofilms Exam > Flashcards

P. aeroginosa Biofilms Exam Flashcards

1
Q

Where do you find P aeroginosa?

A

The muscous mebrane in the lungs and other parts of the body

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2
Q

What sre the ozygen requirements of the P. Aeriginosa

A

Faculatative aerobic

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3
Q

What are the two serotypes of the flagella of P. Aeruginosa?

A

Serotype A and serotype B

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4
Q

What is doin with the flagella of P. aeroginosa?

A
  • It has two serotypes
  • immunogenic hook
  • associated proteins
  • When the flagella is lost, there is less chance of the host immune system ID-ing the P. aeruginosa.(Zhang et al 2007)
  • Contact with cystic fibrosis fluids the flagella become down-regulated
  • Function: cell-to-cell signalling, signal transduction
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5
Q

What are the fimbriae on the P aeroginosa?

A

Cup A-C gene cluster fimbriae Adherence factors

TYPE IV Pilus: mediates adhesion and translocation to epithelial cells allows an independent twitch movement independent of the flagella

Adhesion factors: colonisation factors and protective antigens

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6
Q

What does c-di-GMP do in P. aeruginosa?

A

makes the ole biofilms

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7
Q

What mobile genetic elements does P. aeruginosa have?

A

conjugative plasmid

Gene islands which are highly capable of horizontal gene transfer

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8
Q

What shape is the P. aeruginosa in the wild, aquatic, and lab

A

fingers

smol fingers

mushroom

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9
Q

What are the two pol;saccharides of the Alginate?

A

glumuronic acid and mannuroic acid

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10
Q

What is the seconds most common polysachharide produces in the EPS of the P. aeroginosa?

A

PSL: Polysacharide

repeating pentemer of mannose, phamnose, glucose

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11
Q

What population benifits from PSL: Polysacharide?

A

Thise who make it and have the genes

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12
Q

What can Paerogunosa bleb?

A

eDNA

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13
Q

How does P aeruginosa detach from the biofilm?

A

Aliginate lysase: algL

Rhampolipids: surficants

Cis-2-deconoic acids

DN-ase

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14
Q

What are the three Quorum sensing pathways of the P. aeruginosa?

A

LasI/LasR

RhlI/RhlR

Pseudomonas quinolone Signalling

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15
Q

What are the Proteins involved in the LasI/LasR Pathway?

A

c12-HSL

LasR

QscR

LasI

RhlR

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16
Q

What are the stages of the LasI/LasR quorum sensing pathways?

A
  1. C12-HSL binds to the receptor of the P. aeruginosa
  2. C12-HSL enters the cytoplasm of the P. aeruginosa
  3. LasR autoinducer complex binds to the promoter
  4. Upregulates Virulence factors: LasI
  5. LasI makes some C12-HSL: upregulates RhlR
17
Q

What are the stages of the LasI/LasR quorum sensing pathways when you bind to QscR?

A
  1. C12-HSL binds to the receptor of the P. aeruginosa
  2. C12-HSL enters the cytoplasm of the P. aeruginosa
  3. C12-HSL binds to QscR in low cell density
  4. QcsR binds promoter to reduce Lasl/LasR and RhlI/RhlR operons
18
Q

What virulence factors are upregulated by the LasI/LasR Quorum sensing pathway?

A
  • LasI•Protease (lasA)
  • Elastase (lasB)
  • Exotoxin A (toxA)
  • Alkaline phosphatase (aprA)
19
Q

What proteins are involved in the RhlI/RhlR Quorum Sensing?

A

4-HSL

RhlR

RhIR

20
Q

What are the stages of the RhlI/RhlR Quorum Sensing?

A

C4-HSL binds to the receptor of the P. aeruginosa

C12-HSL enters the cytoplasm of the P. aeruginosa

C4-HSL binds to RhlR creating the RhlR autoinducer complex

LasR autoinducer complex binds to the promoter.

Upregulates Virulence factors RhlI

RhlI makes some C4-HSL

21
Q

What do all the three qurum sensing pathways of aerogunosa regulate?

A

Elastase production

22
Q

What is the PQS involved in regulating?

A

maturation through releasing blebbed eDNA.

elastase production

23
Q

What is the PQS system not in charge of regulating?

A

Not involved in sensing cell density

24
Q

What proteins are involved in the Pseudomonas quinolone signal (PQS)

A

PQS

PQS-R

PQS-I

25
Q

What are the stages of Pseudomonas quinolone signal (PQS)?

A

PQS binds to the receptor of the P. aeruginosa

PQS enters the cytoplasm of the P. aeruginosa

PQS binds to PQSR creating the LasR autoinducer complex

PQS-R autoinducer complex binds to the promoter.

Upregulates Virulence factors: PQSI

PQS-I makes some PQS

26
Q

What virulence factors are involved with Pseudomonas quinolone signal (PQS)?

A

•Partially controls, depending on physiological states is required to work in tandem with the other systems…. the lasB elastase and the pyocyanin genes regulated•PQS-I

27
Q

What are the virulence factors involved with RhlI/RhlR Quorum Sensing

A

•RhlI•rpoS – encodes stationary phase sigma factor•rhlAB – encodes rhamnosyltransferase (rhamnolipid production)•lecA – encodes a cytotoxic lectin•RhlI – autoregulatory feedback loop•Genes encoding pyocynain•Antibiotic•Oxides and reduces other molecules•lasB (elastase)•aprA (alkaline phosphatase)

OMICS (11 decks)

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