Oxidative Phosphorylation Uncouplers

Question 1

Synthetic Uncouplers

Answer 1

• Acts by destroying Proton gradient

- Includes: DNP (2, 4 Dinitrophenol) & ASA (Aspirin)

Question 2

Ionophores

Answer 2

• Gramicidin (Channel forming)

- Valinomycin (Mobile barrier)

Question 3

Uncoupling Proteins

Answer 3

UCP1/Thermogenin

Question 4

Uncouplers

Answer 4

• Decrease ATP synthesis, Increase ETC & O2 consumption

- Includes: Synthetic Uncouplers, Ionophores, Uncoupling Proteins

Question 5

Valinomycin

Answer 5

• Mobile Ionophore
• Antibiotic
• Makes inner membrane permeable to K+
• Dissipates membrane potention/proton motive force

Question 6

Gramicidin

Answer 6

• Channel forming ionophore
• Peptide mix which form helices that span lipid bilayers
• Makes a pore through IMM & makes it permeable to H+

Question 7

UPC1/Thermogenin

Answer 7

• Uncoupling Protein
• Expressed in brown adipose tissue
• Heat generation
• Brown pigment due to Cyt-containing mitochondria
• Biological heating pad in infants (neck & upper back)