Oxidative Phosphorylation and the Electron Transport Chain

Question 1

Name the 5 carriers of the e- transport chain (in order)

Answer 1

1. NADH dehydrogenase, aka coenzyme Q reductase
2. Ubiquinone, aka coenzyme Q
3. Cytochrome C reductase
4. Cytochrome C
5. Cytochrome C oxidase

Question 2

Oxidative phosphorylation refers to both ____ transport chain and ____ production

Answer 2

e-

ATP

Question 3

when H+ are pumped across the inner mitochondrial membrane out of the matrix and into the intermembrane space, this creates a ____ pH in the intermembrane space

Answer 3

low (high acidity)

Question 4

What is the large protein complex embedded in the inner membrane space that allows passage of H+ from the intermembrane space back into the matrix, allowing ADP to be turned into ATP?

Answer 4

ATP Synthase

Question 5

How many H+ are used to make 1 ATP molecule?

Answer 5

4

Question 6

How many ATP are generated per NADH and FADH2?

Answer 6

2. 5 ATP per NADH

1. 5 ATP per FADH2

Question 7

How many ATP are generated per glucose molecule in glycolysis, PDC, Krebs, and altogether?

Answer 7

glycolysis: 2 net ATP directly, 3 ATP from it’s NADH (cytosol NADH only generates 1.5 ATP per NADH)
PDC: 5 ATP (from 2 NADH)
Krebs: 15 ATP from NADH, 3 ATP from FADH2, 2 ATP from GTP
Total: 30 for eukaryotes (32 for prokaryotes)

Question 8

The e- transport chain in the inner mitochondrial membrane starts with the __1__ of the e- carriers NADH and FADH2, and ends with the reduction of __2__ and the generation of a __3__ gradient across the inner mitochondrial membrane.

Answer 8

1. oxidation
2. oxygen
3. proton (H+)

Question 9

How many ATP are produced from 1 cytoplasmic NADH from glycolysis? Why?

Answer 9

1.5 ATP because cytoplasmic NADH drop their e- off at the second e- carrier (like FADH2 do) instead of the first

Question 10

Where do prokaryotes perform their e- transport chain?

Answer 10

prokaryotes use their cell membrane for their e- transport chain