Glycolysis

Question 1

List the major intermediate compounds of glycolysis, starting with glucose and ending with pyruvate/

Answer 1

Glucose, Glucose-6-Phosphate (G6P), Fructose-6-Phosphate (F6P), Fructose-1,6-bisphosphate (F-1,6-bP), glyceraldehyde-3-P (GAP), Phosphoenolpyruvate (PEP), pyruvate

Question 2

List functions of hexokinase, phosphofruktokinase (PFK), and pyruvate kinase in glycolysis.

Answer 2

hexokinase converts glucose to G6P
PFK converts F6P into F1,6bP
Pyruvate kinase converts PEP into pyruvate

Question 3

What are the regulatory steps of glycolysis?

Answer 3

mainly hexokinase and phosphofructokinase (less so pyruvate kinase)

Question 4

Which steps in glycolysis have a very negative delta G?

Answer 4

hexokinase, PFK, pyruvate kinase

Question 5

what is hexokinase inhibited by?

Answer 5

6C-P

Question 6

What is PFK inhibited by and stimulated by?

Answer 6

inhibited by ATP

stimulated by F-2,6-bP (which is stimulated by insulin and inhibited by glucagon)

Question 7

Name 2 positive regulators of PFK and one negative regulator of PFK

Answer 7

positive = F26BP and AMP
negative = ATP