Oxidative Phosphorylation

Question 1

Catabolic Pathway Purposes

Answer 1

Breakdown larger molecules into smaller building units, release and storage of energy in high-energy molecules

Question 2

cataobolic pathway cofactors

Answer 2

reduced cofactors (NADH/FADH2)

Question 3

Catabolism Ox or Red

Answer 3

Oxidative. Metabolites are oxidized whiel cofactors are reduced.

Question 4

Re-oxidation of cofactors (catabolism)

Answer 4

used to generate ATP

Question 5

Oxidative Phsophorylation

Answer 5

separate but connected process, linked through a proton gradient across the mitochondrial membrane

Question 6

Oxidation

Answer 6

oxidation of reduced cofactors (NADH, FADH2) and reduction of molecular oxygen

Question 7

Phosphorylation

Answer 7

phosphorylation of ADP to ATP

Question 8

Overview of oxidative phosphorylation

Answer 8

reduced cofactors generated form glycolysis -> electron teransport chain -< prton gradient -> ATP synthesis

Question 9

Location of oxidative phosphorylation

Answer 9

Inner Mitochondrial membrane

Question 10

how many protons do you need for ATP synthesis (approx)

Answer 10

3

Question 11

Two portions of ATP synthase

Answer 11

F0 and F1

Question 12

Fo

Answer 12

Transmembrane portion, protons pass through, triggers conformation change in F1

Question 13

F1

Answer 13

catalytic protion, synthesis of ATP from ADP and Pi

Question 14

What does the rate of ATP synthase determine

Answer 14

Proton movement and (ultimately) oxygen consumption

Question 15

adenine nucleotide translocation and the Pi-H+ Symport

Answer 15

exports newly syntheised ATP into the cytsol and ADP + Pi produced in the cytosol into the mitochondrial matrix

Question 16

coupling (in Oxidative phosphorylation and ATP synthesis)

Answer 16

It is a coupled process
(as one increases so does the other)

Question 17

how many water are made for 1 NADH or FADH2 reoxidized

Answer 17

1 H2O (each 2e-)

Question 18

P:O ratio for FADH2 reoxidized

Answer 18

1.5

Question 19

P:O ration for NADH reoxidived

Answer 19

2.5

Question 20

ADP increases (effect on O2)

Answer 20

O2 consumption increases

Question 21

What is uncoupling

Answer 21

allows H+ to enter matrix without ATP, generating heat in the process

Question 22

Uncoupling effect on O2

Answer 22

electron transport occurs without ATP synthesis