Glycolysis Flashcards
Glycolysis Cat or Ana
Catabolic pathway
Glycolysis Overview
1 molecule of glucose into 1 molecule of pyruvate, generates ATP directly and NADH from oxidation of metabolites.
glucose to pyruvate
glycolysis
pyruvate to glucose
gluconeogensis
Does glycolysis require O2
NO, it is anaerobic
(can operate anaerobically)
stage 1
energy investment (hexose)
stage 2
energy pay off (triose)
hexokinase catalyzed rxn
irreversable, exergonic, coupled, phosphate transfer, regulated (but not rate limiting)
PFK-1 cataylzed rxn
irreversable, exergonic, coupled, phosphate transfer, regulated, rate limiting
glyceraldyehyde-3-phosphate dehydrogenase cat. rxn
oxidation, reversible, “energy capture”
1,3-BPG to 3-phosphoglycerate
reverisble, coupled, “energy capture” step, substrate-level phosphorylation
pyruvate kinase cat. rxn
irriversible, coupled, substarate level phosphorylation, regulated, energy capture step
4 things affecting flux rate
(general metabolic processes)
substrate availability, aleration of enzyme activity, alteration of amount of enzyme, compartmentation
regulation in glycolysis
substrate availability (glucose import), and enzyme regulation (Hexiokinase, PFK-1, Pyruvate kinase)
Aerobic reoxidation of NADH back to NAD+
oxidative phosphorylation
Anareobic reoxidation of NADH to NAD+
Pyruvate reduction (ethanol, lactate formation)
why does pyruvated get converted to lactate
to regentate NAD+ to NADH
what transport pyruvate into the mitochdria matrix
pyruvate translocase
PDH regulation (dephosphorylation)
swithces activoty off via kinase
PDH regulation (phosphorylation)
activates the complex via a phosphotase
PDH regulation substrate activation
NAD+ and HS-CoA
PDH regulation substarte inhibition
NADH and acety;-CoA
