Oxidative Phosphorylation

Question 1

Complex V has 14 subunits and goes across the inner mitochondrial membrance to connect the mitochondrial matrix and intermembranous space. What makes it spin and what reaction is happening?

Answer 1

Hydrogen (H⁺) passes through complex V (ATP synthase) to make the complex spin. This then helps ADP and phosphate to become ATP.

ATP synthase is the rotary motor

Question 2

Where does oxidative phosphorylation occur?

Answer 2

Entirely in the inner
membrane of the mitochondria

Question 3

What is the goal of oxidative phosphorylation?

Answer 3

to produce ATP through the phosphorylation of ADP

Question 4

We want to harvest NADH (energy) in pieces so taht it is most efficient.

Answer 4

I think this is why there are multiple complexes to bring H⁺ in to the intermembranous space.

Question 5

Which complexes are responsible for for bringig H⁺ ions in to the intermembranous space?

Answer 5

complex I
complex III
complex IV

Question 6

How many subunits make up complex I?

Answer 6

40 subunits

Question 7

How many subunits make up complex III?

Answer 7

9 subunits

Question 8

How many subunits make up complex IV?

Answer 8

13 subunits

Question 9

What does complex I do?

Answer 9

• NADH enters complex 1 and interacts with FMNH2 and results in the products of 4 protons and coenzyme Q

Question 10

What does complex II do?

Answer 10

is in the mitochondrial matrix and is part of the TcA cycle
enzyme = succinate dehydrogenase
FADH -> ETF.CoQ

Question 11

What does complex III do?

Answer 11

2H⁺ interacts with coenzyme Q and spits out of 4H⁺ and cytochrome C

cytochrome C is rish in iron

Question 12

What is cytochrome C rich in?

Answer 12

iron

Question 13

What does complex IV do?

Answer 13

In the mitochondrial matrix hydrogen and oxygen come together and make water, while the other products enter complex IV with cytochrome C pump 2 H⁺ ions in to the intermembranous space