Enzyme Kinetics Flashcards
The KM of an enzyme represents…
substrate affinity
Which one of the following represents a way of measuring the rate of an enzymatic reaction?
A. Change in the concentration of a substrate as a function of time
B. Mean value between initial velocity and maximum velocity
C. Ratio of the forward and backward reaction rate constants
D. The ratio of concentrations of product over substrate at the equilibrium point of a reversible reaction.
E. Turnover rate of an enzyme.
A. Change in the concentration of a substrate as a function of time
What is the algebraic relationship between the initial velocity (V0) of a reaction and the enzyme concentration?
A. Exponential
B. Hyperbolic
C. Independent
D. Linear
E. Sigmoid
D. Linear
The rate of an enzymatic reaction is not dependent on which one of the following?
A. concentration of substrate
B. concentration of enzyme
C. difference in free energy between the substrates and the products
D. pH
E. Temperature
C. difference in free energy between the substrates and the products
Which one of hte following is not part of the Michaelis Menden equation?
A. [E] (enzyme concentration)
B. Km (Michaelis constant)
C. [S] (substrate concentration)
D. V0 (initial reaction velocity
E. Vmax (maximal reaction velocity)
A. [E] (enzyme concentration)
True or False?
In the Michaelis-Menten equation, the original definition of KM is contingent upon the assumption that early in the enzyme reaction, the concentration of the product is so low that the rate of backward conversion of the product toenzyme-substrate intermediates (EScomplex) is negligible.
True