Other Technologies and Automation Flashcards
Which technology has the following advantages:
- AHG testing can be performed without multiple saline washes
- Check cells are not needed to check for AHG reagent added for negative results
Gel technology
Gel technology results are valid for how long?
up to 3 days
In gel technology, what is located in the reaction chamber?
reagents (including patient sample when added)
In gel technology, where is the gel located?
in the column
TRUE OR FALSE: Gel technology provides a more stable endpoint, more reproducible results, and reduces the variability associated with the physical resuspension of RBC buttons.
TRUE
ABO forward grouping cards contain gel that contain…
anti-A, anti-B, and anti-A,B
Which antisera can help detect subgroups of type A and/or B?
anti-A,B
The Rh typing card contains gel that contains
anti-D
The Rh phenotyping card contains what in the gel?
anti-D, anti-C, anti-c, anti-E, anti-e, and a control
For compatibility testing, antibody detection/ID, the microtubes are filled with gel that contain what?
anti-IgG
What kind of gel is used in the gel technology cards?
dextran-acrylamide gel
A mixed-field result in gel technology may be seen when?
post transfusion
TRUE OR FALSE: Rouleaux may cause a problem when running gel technology.
TRUE
What is the difference between 1st and 2nd generation Solid-Phase Red Cell Adherence (SPRCA) tests?
1st generation: target antigen is added by the user
2nd generation: target antigen is bound to microplates by the manufacturer
A monolayer of RBC coating the bottom of the microplate is what type of result?
positive
Protein A is a cell wall component of which organism?
Staphylococcus aureus
Protein A has high affinity to what portion of most Ig?
Fc portion
TRUE OR FALSE: HLA antibodies (Class 1 and 2) can be screen for via ELISA methods.
TRUE
Which solid-phase method is used to screen and identify platelet antibodies?
Solid-Phase ELISA
The solid-phase ELISA test (MACE) is used primarily for
compatibility testing
Which solid-phase product provides well-characterized platelet glycoproteins that are either immobilized directly or captured my monoclonal antibodies to wells of a micro well plate?
PAK products
Solid-phase PAK products allow for detections and differentiations of…
antibodies that bind to platelet-specific glycoproteins
Gel technology can detect which classes of Ig?
IgG and IgM
SPRCA can only detect which class of Ig?
IgG
Do SPRCA or gel technology detect complement?
no
Which method requires washing, solid-phase and/or gel technology?
solid-phase
Which technology utilizes both protein A and protein G?
Affinity column
Which technology utilized a beaded gel matrix?
Affinity column
Which protein binds to the Fc portion of all IgG subclasses?
Protein G
Which protein binds to the Fc portion of all IgG subclasses, except IgG3
Protein A
