Other Stains

Question 1

a mixture of picric acid and acid fuchsin for demonstration of connective tissues

Answer 1

Acid Fuchsin-Picric Acid (Van Gieson’s Stain)

Question 2

used to stain collagen, smooth muscle, or mitochondria; used as the nuclear and cytoplasmic stain in Mallory’s trichrome method

Answer 2

Acid Fuchsin (Masson Stain)

Question 3

traditional stain for mitochondria

Answer 3

Acid fuchsin

Question 4

permits discrimination between dead and living cells

Answer 4

acridine orange

Question 5

green fluorescence

Answer 5

DNA

Question 6

red fluorescence

Answer 6

RNA

Question 7

used to demonstrate deposits of CALCIUM SALTS and
possible sites of phosphatase activities.

Answer 7

ACRIDINE RED 3B

Question 8

a complex, water-soluble phthalocyanin dye; similar to chlorophyll that stains acid mucopolysaccharides

Answer 8

alcian blue

Question 9

used on the Dupont ACA analyzer to measure serum calcium photometrically

Answer 9

Aliizarin Red S

Question 10

stain used for counterstaining of epithelial sections

Answer 10

ANILINE BLUE

Question 11

plasma stain utilized also for deep staining of acid fast organisms, for mitochondria, for differentiation of smooth muscles

Answer 11

basic fuchsin

Question 12

used for staining hemoglobin

Answer 12

Benzidine

Question 13

used as a contrast stain for Gram’s technique; for staining diphtheria organisms

Answer 13

Bismark brown

Question 14

used as a chromatin stain for fresh materials

Answer 14

Carmine (carminic acid)

Question 15

mordanted dye acting as a basic dye and staining acidic substances

Answer 15

CARMALUM (MAYER’S) SOLUTION

Question 16

an oxazine dye used as an alternative to iron hematoxylin nuclear stain

Answer 16

CELESTINE BLUE

Question 17

best known as an indicator; stain for axis cylinders in embryos; AMYLOID

Answer 17

CONGO RED

Question 18

used in histology to stain nervous tissues

Answer 18

Cresyl violet

Question 19

nuclear or chromatin stain used for staining amyloid in frozen sections and platelets in blood

Answer 19

crsytal violet

Question 20

solution formed by the mixture of crystal violet, methyl violet and dexterin

Answer 20

Gentian violet

Question 21

stains DNA, providing a fluorescent red-orange stain; used to identify cells that are in the final stages of apoptosis

Answer 21

ETHIDIUM BROMIDE

Question 22

mixture of methylene-blue and eosin; staining blood to differentiate leukocytes

Answer 22

giemsa stain

Question 23

binds to spirochetes, trypanosomes and bacteria

Answer 23

giemsa stain

Question 24

stain used for metallic impregnation, made up of gold chloride and mercuric chloride

Answer 24

Gold sublimate

Question 25

oldest of all stains; stains amyloid, cellulose, starch, carotenes and glycogen

Answer 25

IODINE

Question 26

used to identify and differentiate bacteria

Answer 26

Grams Iodine

Question 27

a brown solution that turns black in the presence of starches

Answer 27

Lugols iodine

Question 28

used as a mordant in Gram's staining

Answer 28

lugols iodine

Question 29

used for demonstrating mitochondria during intravital staining

Answer 29

Janus green B

Question 30

staining erythrocytes, ascaris egg, bacterial spores; both decolizer and counterstain

Answer 30

malachite green

Question 31

stains chromatin green in bright field microscope

Answer 31

Methyl Green

Question 32

common basic nuclear stain

Answer 32

methylene blue

Question 33

The resulting mixture of methylene blue, azures and thiazoles is known

Answer 33

polychrome methylene blue

Question 34

metachromatic dye; coloring nuclei of leukocytes reddish-purple in the presence of methylene blue

Answer 34

methylene violet

Question 35

metachromatic dye; coloring nuclei of leukocytes reddish-purple in the presence of methylene blue

Answer 35

methylene violet

Question 36

formed by boiling Nile blue with sulfuric acid; LIPOPHILIC STAIN

Answer 36

nile red

Question 37

dye that is more soluble in fat; stain for neutral fat

Answer 37

OIL RED O

Question 38

excellent stain for elastic fibers

Answer 38

orcein

Question 39

demonstrate the finest and most delicate fibers in the skin

Answer 39

orcein

Question 40

stain for unsaturated lipids and for lipoproteins such as myelin (black)

Answer 40

Osmic oxide

Question 41

all-around useful stain; stains glycogen, mucin, mucoprotein, glycoprotein

Answer 41

Periodic Acid Schiff

Question 42

demonstration of connective tissue

Answer 42

Picric acid

Question 43

used for microanatomical color contrast of specimens; demonstration of the blood and lymph vessels by injection (intravital staining)

Answer 43

Prussian blue

Question 44

stain blood and glandular tissues

Answer 44

Rhodamine B

Question 45

used primarily as a counterstain; produce red nuclei and yellow color to collagen

Answer 45

Safranin

Question 46

identification of spirochetes, reticulum and other fiber stains.

Answer 46

Silver nitrate

Question 47

used for demonstration of neuroglia in frozen sections

Answer 47

victoria blue

Question 48

four major staining properties of WRIGHT STAIN

Answer 48

basophilia (methylene blue) azuphilia acidophilia (eosin) neutrophilia