OIL SOLUBLE DYES Flashcards
not real dyes
lysochromes
used for the demonstration of intracellular fats
Sudan III
Sudan IV
In order to penetrate fats, the oil soluble dyes (Sudan dyes) must be
dissolve of the oil in soluble dye
it has a much greater affinity for phospholipids
sudan black
stain that colors fat droplets black and is the most
SENSITIVE of the oil soluble dyes
SUDAN BLACK
sudan black imparts _____ on intracellular lipids
black color
recommended for paraffin sections especially for tissues fixed in formol calcium with post chroming
Sudan Black
demonstrate lipids that are resistant to paraffin embedding
Sudan black
stains phospholipids as well as neutral fats
sudan black B
does not stain crystalline cholesterol, and free fatty acids
sudan black B
it has no secondary amino group and it does not color phospholipids
sudan IV
Addition of ____________ intensifies fat and prevents rapid deterioration of the solutions
BENZOIC ACID
first Sudan dye; good as a fat stain for central nervous system tissues
Sudan III
giving a less deep and lighter orange stain
SUDAN III
CHEIF SOLVENTS USED FOR STAINS
water
alcohol
aniline water
phenol