Common Staining Solutions

Question 1

staining solution most commonly used for routine histologic studies

Answer 1

hematoxylin

Question 2

the color of hematoxylin is depended on what salt

Answer 2

Aluminum salt - blue
ferric salt - blue black

Question 3

most commonly used staining system

Answer 3

H&E

Question 4

T/F
hematoxylin (basic): stain acidic
eosin(acidic): stain basic

Answer 4

TRUE

Question 5

recommended for progressive staining of tissues; can be used in regressive staining

Answer 5

aluminum hematoxylin stain

Question 6

the procedure in which the neutralize the acid and free the OH group, to form an insoluble blue aluminum hematin-tissue-lake

Answer 6

blueing

Question 7

in blueing alum-hematoxylin, what is the commonly used

Answer 7

warm tap water

Question 8

water is not sufficiently alkaline, what may be used

Answer 8

lithium carbonate
bicarbonate
potassium/sodium acetate

Question 9

used to accelerate blueing of thin paraffin sections

Answer 9

Scott’s Tap Water Substitute

Question 10

may be “hard” on delicate tissues and may loosen and cause sections to fall off the slides during staining.

Answer 10

ammonia

Question 11

has a tendency to form crystalline deposits unless the slides are agitated in it and washed well afterwards.

Answer 11

lithium carbonate

Question 12

slows down the process of blueing; sections may even produce pink artifact discolorations on the tissue

Answer 12

cold water

Question 13

acts as a stabilizer, retards evaporation of the solution, and appears to slow down ripening

Answer 13

glycerin

Question 14

not an ideal stain for frozen sections

Answer 14

ehrlich hematoxylin

Question 15

good regressive stain that may either be used immediately or stored for future use

Answer 15

harris hematoxylin

Question 16

widely used for routine nuclear staining, in exfoliative cytology, and for staining of sex chromosomes

Answer 16

harris hematoxylin

Question 17

another alum hematoxylin solution recommended for routine purposes; used in sequence with CELESTINE BLUE

Answer 17

cole’s hematoxylin

Question 18

alum hematoxylin that is chemically ripened with sodium iodate; used as a regressive stain, but it is also
useful as a progressive stain

Answer 18

Mayer’s hematoxylin

Question 19

used in Celestine Blue hemalum method of nuclear staining

Answer 19

mayer’s hematoxylin

Question 20

used only for differential or regressive staining

Answer 20

Iron Hematoxylin

Question 21

Main Iron hematoxylin solutions employed for routine work

Answer 21

Weigert’s solurtion
Heidenhain’s solution

Question 22

Tissue structures are stained blackish or grayish; producing minimal eyestrain and useful for photomicrography

Answer 22

Iron hematoxylin

Question 23

permanent and simplest in demonstrating mitochondria by LM

Answer 23

Regaund’s Hematoxylin

Question 24

standard iron hematoxylin stain used in the laboratory; demonstrating muscle fibers and connective tissues

Answer 24

Weigert’s solution

Question 25

a popular cytological stain, especially for the study of mitosis; all components are black or dark grey - black.

Answer 25

Heidenhain’s hematoxylin

Question 26

demonstrates structures in paraffin as well as celloidin and frozen sections

Answer 26

Phosphotungstic acid hematoxylin stain

Question 27

stains used for differentially staining connective tissues and cytoplasm; hemoglobin gives ORANGE color

Answer 27

EOSIN

Question 28

routinely used in histopathology as a counterstain to hematoxylin

Answer 28

EOSIN

Question 29

what eosin is commonly used

Answer 29

Eosin Y

Question 30

Eosin that has a very faint bluish cast

Answer 30

Eosin B

Question 31

Romanowsky stain is combination of what?

Answer 31

eosinate and methylene blue

Question 32

used to examine blood or bone marrow samples; o detect blood-borne parasites like malaria.

Answer 32

Romanowsky stain

Question 33

variants of Romanowsky stain

Answer 33

Wright stain
Jenner Stain
Leishman
Giemsa