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MTAP2-FINALS-HISTO > Natural Dye > Flashcards

Natural Dye Flashcards

1
Q

Natural dyes are those obtained from

A

plants and animals

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2
Q

common natural dyes available

A

hematoxylin
cochineal dye
orcein
saffron

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3
Q

most valuable staining reagent used by the cytologist due to its powerful nuclear and chromatin staining capacity

A

hematoxylin

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4
Q

Hematoxylin is a natural dye derived by extraction from the core or the heartwood of a Mexican tree known as

A

hematoxylin campechianum

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5
Q

T/F:
Hematoxylin itself is a true basic dye

A

false

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6
Q

hematoxylin active coloring

A

hematin

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7
Q

process where hematin is formed by oxidation of hematoxylin

A

ripening

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8
Q

strong oxidizing agent that can accelerated ripening

A

hydrogen peroxide
mercuric oxide
potassium permanganate
sodium perborate or sodium iodate

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9
Q

substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction to take place

A

Mordant

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10
Q

acts as mordant in hematoxylin

A

alum
iron
chromium
copper salt

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11
Q

recommended for progressive staining of tissues

A

Alum hematoxylin stain

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12
Q

alum counterstains

A

eosin
congo red
safranin

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13
Q

solutions that contain alum hematoxylin

A

ehrlich solution
harris solution

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14
Q

Harris: Mecuric chloride
Ehrlich: ____

A

sodium iodate

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15
Q

used only for differential or regressive staining

A

Iron Hematoxylin Stain

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16
Q

Iron Hematoxylin differentiating agent

A

acid alcohol

17
Q

example of Iron Hematoxylin

A

Weigert’s Stain using Iron (Ferric) Chloride

18
Q

utilized for the study of spermatogenesis

A

Copper Hematoxylin solution

19
Q

used frequently in histology to examine thin sections of tissue

A

Hematoxylin and Eosin

20
Q

stains cell nuclei

A

hematoxylin

21
Q

stains cytoplasm, connective tissue, collagen and other extracellular substances

A

eosin

22
Q

widely used as a powerful chromatin and nuclear stain for fresh material and smear preparations

A

Cochineal dye

23
Q

Cochineal dye is an old histologic dye extracted from the female cochineal bug ______________

A

Coccus Cacti

24
Q

extensively used in neuropathological studies

A

Cochineal + picric acid (picrocarmine)

25
Q

used for the demonstration of glycogen

A

cochineal + aluminum chloride (Best carmine stain)

26
Q

vegetable dye extracted from certain lichens which are normally colorless

A

Orcein

27
Q

color produce if orcein is treated with ammonia and exposed to air

A

blue or violet

28
Q

used for staining elastic fibers

A

orcein

29
Q

also obtained from lichens, treated with lime and soda, and exposed to ammonia and air

A

lichens

30
Q

used mainly as an indicator

A

lichens

31
Q

T/F:
used as a cytological stain because of its high staining property

A

False (not used; poor staining)

MTAP2-FINALS-HISTO (5 decks)

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