Natural Dye Flashcards
Natural dyes are those obtained from
plants and animals
common natural dyes available
hematoxylin
cochineal dye
orcein
saffron
most valuable staining reagent used by the cytologist due to its powerful nuclear and chromatin staining capacity
hematoxylin
Hematoxylin is a natural dye derived by extraction from the core or the heartwood of a Mexican tree known as
hematoxylin campechianum
T/F:
Hematoxylin itself is a true basic dye
false
hematoxylin active coloring
hematin
process where hematin is formed by oxidation of hematoxylin
ripening
strong oxidizing agent that can accelerated ripening
hydrogen peroxide
mercuric oxide
potassium permanganate
sodium perborate or sodium iodate
substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction to take place
Mordant
acts as mordant in hematoxylin
alum
iron
chromium
copper salt
recommended for progressive staining of tissues
Alum hematoxylin stain
alum counterstains
eosin
congo red
safranin
solutions that contain alum hematoxylin
ehrlich solution
harris solution
Harris: Mecuric chloride
Ehrlich: ____
sodium iodate
used only for differential or regressive staining
Iron Hematoxylin Stain
Iron Hematoxylin differentiating agent
acid alcohol
example of Iron Hematoxylin
Weigert’s Stain using Iron (Ferric) Chloride
utilized for the study of spermatogenesis
Copper Hematoxylin solution
used frequently in histology to examine thin sections of tissue
Hematoxylin and Eosin
stains cell nuclei
hematoxylin
stains cytoplasm, connective tissue, collagen and other extracellular substances
eosin
widely used as a powerful chromatin and nuclear stain for fresh material and smear preparations
Cochineal dye
Cochineal dye is an old histologic dye extracted from the female cochineal bug ______________
Coccus Cacti
extensively used in neuropathological studies
Cochineal + picric acid (picrocarmine)
used for the demonstration of glycogen
cochineal + aluminum chloride (Best carmine stain)
vegetable dye extracted from certain lichens which are normally colorless
Orcein
color produce if orcein is treated with ammonia and exposed to air
blue or violet
used for staining elastic fibers
orcein
also obtained from lichens, treated with lime and soda, and exposed to ammonia and air
lichens
used mainly as an indicator
lichens
T/F:
used as a cytological stain because of its high staining property
False (not used; poor staining)
