Natural Dye Flashcards
Natural dyes are those obtained from
plants and animals
common natural dyes available
hematoxylin
cochineal dye
orcein
saffron
most valuable staining reagent used by the cytologist due to its powerful nuclear and chromatin staining capacity
hematoxylin
Hematoxylin is a natural dye derived by extraction from the core or the heartwood of a Mexican tree known as
hematoxylin campechianum
T/F:
Hematoxylin itself is a true basic dye
false
hematoxylin active coloring
hematin
process where hematin is formed by oxidation of hematoxylin
ripening
strong oxidizing agent that can accelerated ripening
hydrogen peroxide
mercuric oxide
potassium permanganate
sodium perborate or sodium iodate
substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction to take place
Mordant
acts as mordant in hematoxylin
alum
iron
chromium
copper salt
recommended for progressive staining of tissues
Alum hematoxylin stain
alum counterstains
eosin
congo red
safranin
solutions that contain alum hematoxylin
ehrlich solution
harris solution
Harris: Mecuric chloride
Ehrlich: ____
sodium iodate
used only for differential or regressive staining
Iron Hematoxylin Stain