Natural Dye

Question 1

Natural dyes are those obtained from

Answer 1

plants and animals

Question 2

common natural dyes available

Answer 2

hematoxylin
cochineal dye
orcein
saffron

Question 3

most valuable staining reagent used by the cytologist due to its powerful nuclear and chromatin staining capacity

Answer 3

hematoxylin

Question 4

Hematoxylin is a natural dye derived by extraction from the core or the heartwood of a Mexican tree known as

Answer 4

hematoxylin campechianum

Question 5

T/F:
Hematoxylin itself is a true basic dye

Answer 5

false

Question 6

hematoxylin active coloring

Answer 6

hematin

Question 7

process where hematin is formed by oxidation of hematoxylin

Answer 7

ripening

Question 8

strong oxidizing agent that can accelerated ripening

Answer 8

hydrogen peroxide
mercuric oxide
potassium permanganate
sodium perborate or sodium iodate

Question 9

substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction to take place

Answer 9

Mordant

Question 10

acts as mordant in hematoxylin

Answer 10

alum
iron
chromium
copper salt

Question 11

recommended for progressive staining of tissues

Answer 11

Alum hematoxylin stain

Question 12

alum counterstains

Answer 12

eosin
congo red
safranin

Question 13

solutions that contain alum hematoxylin

Answer 13

ehrlich solution
harris solution

Question 14

Harris: Mecuric chloride
Ehrlich: ____

Answer 14

sodium iodate

Question 15

used only for differential or regressive staining

Answer 15

Iron Hematoxylin Stain

Question 16

Iron Hematoxylin differentiating agent

Answer 16

acid alcohol

Question 17

example of Iron Hematoxylin

Answer 17

Weigert’s Stain using Iron (Ferric) Chloride

Question 18

utilized for the study of spermatogenesis

Answer 18

Copper Hematoxylin solution

Question 19

used frequently in histology to examine thin sections of tissue

Answer 19

Hematoxylin and Eosin

Question 20

stains cell nuclei

Answer 20

hematoxylin

Question 21

stains cytoplasm, connective tissue, collagen and other extracellular substances

Answer 21

eosin

Question 22

widely used as a powerful chromatin and nuclear stain for fresh material and smear preparations

Answer 22

Cochineal dye

Question 23

Cochineal dye is an old histologic dye extracted from the female cochineal bug ______________

Answer 23

Coccus Cacti

Question 24

extensively used in neuropathological studies

Answer 24

Cochineal + picric acid (picrocarmine)

Question 25

used for the demonstration of glycogen

Answer 25

cochineal + aluminum chloride (Best carmine stain)

Question 26

vegetable dye extracted from certain lichens which are normally colorless

Answer 26

Orcein

Question 27

color produce if orcein is treated with ammonia and exposed to air

Answer 27

blue or violet

Question 28

used for staining elastic fibers

Answer 28

orcein

Question 29

also obtained from lichens, treated with lime and soda, and exposed to ammonia and air

Answer 29

lichens

Question 30

used mainly as an indicator

Answer 30

lichens

Question 31

T/F: used as a cytological stain because of its high staining property

Answer 31

False (not used; poor staining)