Osmotic stress- quiz 4 Flashcards
osmolarity
measure of all solute molecules in sol. moles/L
osmosis
movement of water through membrane
hyposmotic medium (hypotonic)
lower solute outside cell. uses cell wall. bursting.
isosmotic
same solute in/ out of cell. no cell shrinkage
hyperosmotic (hypertonic)
higher solute outside cell. import/synthesize compatible solutes. (potassium/proline) plasmolysis=shriveled.
aquaporin
small pore lined with hydrophobic amino acids. allow only water to pass through.
halophiles
-requre high nacl.
•produce glycerol instead of glycogen
pH
-log(H+)
ph 7= 10^-7 M of protons
-10 fold change less each ph unit
proteins require
normal ph. ph 5-8.5
neutralophiles
ph 5-8, bacteria in gut
acidophiles
ph 0-5. high amounts of tetraether lipids.
alkalophiles
ph 9-11, soda lakes. diether lipids in membrane.
archaeal membrane vs eukaryotes membrane
archaeal have ether bonds.
added what to the plates
cyclohexamide (120 90 disturbed undisturbed)
cells in distilled water …
blow up
cells in salty solution…
shrivel up