Lecture 1

Question 1

How to figure out percent?

given 1%

Answer 1

1%=1gram/100mL
Make 500 mL of 0.4% agar.
0.4g/100 mL * 500 mL = 2g

Question 2

How would you make it?

obtained 2 g from last flashcard

Answer 2

Add 2g agar to 500 mL media, autoclave, cool to 50 degrees C, then pour.

Question 3

Axenic

Answer 3

Pure culture

Question 4

Obtaining pure cultures

Answer 4

•dilution streaking (streak cells on plate)

Flame the loop, cool, add streak

Question 5

Complex media

Answer 5

Usually yeast extract or soybean.

has unknown quantities

Question 6

Defined media

Answer 6

(minimal/mineral media)
•know the contents
•culture oligotrophs - enviro w/ low carbon

Question 7

autoclave

Answer 7

steam cooker
-121 C, 15 psi for 20 min. 
-Kills all bacteria and endospores 
•clostridium botulinum (disease)
    -botulism 
•bacillus anthracis (disease) 
    -anthrax

Question 8

Other methods that kill

Answer 8

•freezing
-will kill some bacteria, not all 
•irradiation 
-uv, x rays, y rays 
•filtration- 0.22mm

Question 9

What happens with detergent?

Answer 9

Dissolves/disrupts the membrane, pulls it apart.

Question 10

What part of meniscus is accurate?

how to keep them sterile?

Answer 10

Bottom.

use proper technique.

Question 11

Plunger has two stops

Answer 11

First- used to measure volume

Second- used to dispense all the liquid

Question 12

buffer

Answer 12

phosphate or potassium source.