Chemical Agents for quiz 5 Flashcards
phenolics
lab and hospital disinfectants-disrupts membrane and denatures proteins
-phenol, amphyl, lysol
alcohols
wont kill endospores, denatures proteins
-isopropanol better than etOH (ethanol)
halogens
disinfectants
-bleach, chlorine, iodine
aldehydes
reactive molecules that act by combining w proteins and inactivating them
sterilized gases
sterilize heat sensitive materials like plastic petri dishes
-ethylene oxide
antibiotics
selectively kills microbes. best ones: minimal effect on eukaryotic cells.
bactericidal antibiotics
kill target organism. ex penicillin, take until all cells leave stationary phase
bacteriostatic antibiotics
prevent growth of organism, cant kill organism
Kirby bauer disk susceptibility test
test strain sensitivity to multiple antibiotics
peptidoglycan antibiotics
penicillin, ampicillin
transpeptidation
adds new peptidoglycan strand
autolysin
cuts peptide stem
quinolones- ciprofloxacin
blocks bacterial dna gyrase, prevents dna replication
macrolides, chloramphenicols, lincosamides
bind to large subunit, block transfer of peptides.
-erythromycin
aminoglycosides
prevents 30s and 50s from binding to each other
-streptomycin
tetracyclines
binds small subunit, blocks binding of aminoacyl-trna.
antibiotic resistance
antibiotics overused (overprescribed)
many bacteria secrete
b-lactamase (exo enyzyme)
phage transfer
transduction
uptake of naked dna
transformation