Bacterophages Quiz 5

Question 1

Replicons

Answer 1

have independent genomes (like plasmids)

Question 2

replicons are different from plasmids bc

Answer 2

they have protein coat for survival outside organisms

Question 3

free state (virion) is

Answer 3

metabolically inert (non living) they need other cells to self replicate. associate w host cells and alter its genetics.

Question 4

__ nm heads, ___nm tails

Answer 4

25-130, approx 500

Question 5

bacteriophage

Answer 5

bacterial viruses. one or more of same type of phage

Question 6

bacteriophageS

Answer 6

more than one type of phage (diff types)

Question 7

plague

Answer 7

cleared area on bacterial lawn

Question 8

virion

Answer 8

free virus particle

Question 9

MOI

Answer 9

multiplicity of infection
ratio of phage/ bacteria.
when you numerate phage, you want a low MOI.

Question 10

all phage must attach and penetrate bacterial envelope to cause entry of genetic material

Answer 10

landing, attachment, tail contraction, penetration, dna injection.

Question 11

a bacterials dna is protected from

Answer 11

its own restriction enzymes by sequence specific methylases that methylate the re so that it doesnt cut the host dna.

Question 12

crispr stands for

Answer 12

clustered regularly interspaced short palindromic repeats

Question 13

spacers are

Answer 13

pieces of past foreign dna

Question 14

palindrome forms a

Answer 14

stem loop in rna-the binding site for CAS protein

Question 15

lytic produces

Answer 15

clear plague, while lysogenic produces cloudy plague

Question 16

some lytic phage can integrate into

Answer 16

host genome and doesnt cause lysis, known as lysogenic

Question 17

sometimes phage are accidentally packed with host dna

Answer 17

enabling lateral gene transfer, “transduction”