organization of cells Flashcards

1
Q

How is mitochondrial DNA inherited?

A

Mitochondrial DNA is inherited only from the mother through maternal inheritance.

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2
Q

What are the two main stages of cellular respiration that occur in mitochondria?

A

The Krebs cycle (in the matrix) and the electron transport chain (on the inner membrane).

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3
Q

Why is the plasma membrane called a “fluid” mosaic?

A

It is called “fluid” because the molecules, such as lipids and proteins, can move freely past one another within the membrane.

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4
Q

What role do vacuoles play in plant cells?

A

Vacuoles maintain proper internal pressure to provide support and structure for growing plant cells

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5
Q

What process involves the breakdown of damaged organelles by lysosomes?

A

This process is called autophagy.

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6
Q

How is illumination provided in Transmission Electron Microscopy (TEM)?

A

Illumination is provided by an electron beam produced by a heated tungsten filament.

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7
Q

What is the key structural difference between the smooth ER and the rough ER?

A

The smooth ER lacks ribosomes on its surface, while the rough ER has ribosomes attached to its membrane

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8
Q

What is the primary function of chloroplasts?

A

Chloroplasts are responsible for photosynthesis, converting light energy into chemical energy stored in glucose.

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9
Q

What does the ribosome do with mRNA?

A

The ribosome moves along the mRNA, translating the genetic message into a protein.

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10
Q

How does the smooth ER help in detoxification?

A

the smooth ER helps liver cells detoxify circulating drugs by modifying harmful substances, making them easier for the body to eliminate.

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11
Q

What roles do proteins play in the plasma membrane?

A

Proteins in the plasma membrane regulate traffic (e.g., transport proteins), assist in signal reception (receptor proteins), and provide structural support and cell recognition.

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12
Q

What are the two main parts of a mitochondrion?

A

The outer membrane and the inner membrane, which is folded into structures called cristae.

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13
Q

What is the tonoplast?

A

The tonoplast is the membrane surrounding the vacuole in plant cells, regulating the movement of materials in and out of the vacuole.

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14
Q

How do vacuoles contribute to cellular function?

A

Vacuoles store water, nutrients, and waste products, and help maintain turgor pressure in plant cells.

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15
Q

What advantage does the Scanning Electron Microscope (SEM) have over TEM?

A

SEM provides great depth of focus and creates 3D images of specimens’ surfaces.

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16
Q

What are the two stages of photosynthesis that occur in chloroplasts?

A

The light-dependent reactions (produce ATP and NADPH) and the light-independent reactions (Calvin cycle, convert CO2 into glucose).

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17
Q

What pigment is found in chloroplasts that helps with photosynthesis?

A

Chlorophyll is the pigment that absorbs light for photosynthesis.

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18
Q

How does a Light Microscope (LM) achieve magnification?

A

Magnification is achieved by the objective lens and ocular (eyepiece) lenses, with illumination provided by light

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19
Q

Why do plants appear green?

A

Plants appear green because chlorophyll reflects green light and absorbs other wavelengths, especially blue and red.

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20
Q

How do proteins from the rough ER reach other parts of the cell?

A

Transport vesicles bud off from the rough ER and carry proteins to other locations, such as the Golgi apparatus.

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21
Q

What are the three main types of RNA involved in protein synthesis?

A

mRNA, tRNA, and rRNA.

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22
Q

What is the function of the nuclear envelope?

A

It separates the nucleus from the cytoplasm and contains pores to regulate material exchange.

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23
Q

What is the structure of microtubules and their role?

A

Microtubules are hollow tubes made of tubulin proteins that help guide the movement of organelles and chromosomes during cell division.

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24
Q

What is the extracellular matrix (ECM) of animal cells made up of?

A

The ECM is composed of glycoproteins such as collagen, proteoglycans, and fibronectin.

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25
Q

What is the role of mRNA in protein synthesis?

A

mRNA carries the genetic code from DNA to the ribosome for protein synthesis

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26
Q

What is the function of lysosomes in eukaryotic cells?

A

Lysosomes break down waste and foreign material within the cell.

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27
Q

What is the function of the vacuole in plant cells related to waste?

A

Vacuoles in plant cells store waste and help break it down, protecting the rest of the plant from harm.

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28
Q

What is the primary function of peroxisomes regarding organic molecules?

A

Peroxisomes use molecular oxygen to oxidize organic molecules.

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29
Q

What is the primary energy currency of the cell?

A

ATP (adenosine triphosphate) is the primary energy carrier in cells.

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30
Q

What does “mosaic” refer to in the fluid mosaic model?

A

“Mosaic” refers to the diverse collection of proteins embedded in the lipid bilayer of the plasma membrane, each performing different functions.

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31
Q

What are glycoproteins?

A

recognition proteins

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32
Q

How do vacuoles function in animal cells?

A

Vacuoles in animal cells are relatively small and primarily involved in storage and waste removal.

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33
Q

How does mRNA exit the nucleus?

A

mRNA exits the nucleus through pores in the nuclear envelope.

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34
Q

What components make up ribosomes?

A

Ribosomes are made up of 4 rRNAs and approximately 80 ribosomal proteins.

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35
Q

What is a key characteristic of prokaryotic cells?

A

They lack membrane-enclosed organelles and a nucleus.

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36
Q

What are the two major categories of cells?

A

Prokaryotic cells and Eukaryotic cells.

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37
Q

What is a nucleosome?

A

A nucleosome is the basic unit of chromatin, consisting of DNA wrapped around 8 histone proteins.

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38
Q

What separates the nucleus from the cytoplasm in a eukaryotic cell?

A

The nuclear envelope, a double membrane that serves as a barrier.

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39
Q

What byproducts are produced during mitochondrial cellular respiration?

A

The byproducts are carbon dioxide (CO2) and water (H2O).

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40
Q

How does the Golgi apparatus work with the endoplasmic reticulum?

A

the Golgi receives products from the ER, modifies them, and then packages them for distribution or secretion

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41
Q

What role do peroxisomes play in inflammation?

A

Peroxisomes assist in the regulation of inflammation, important for immune and tissue repair responses.

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42
Q

What is the structure of a peroxisome?

A

Peroxisomes are bounded by a single bi-lipid layer membrane and do not contain genetic material.

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43
Q

What process do chloroplasts perform to generate energy?

A

Chloroplasts perform photosynthesis, converting light energy into glucose.

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44
Q

What molecule is the final electron acceptor in the mitochondrion’s electron transport chain?

A

Oxygen is the final electron acceptor, forming water when combined with electrons and protons.

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45
Q

What role does collagen play in the ECM?

A

Collagen provides structural strength and resilience to tissues, contributing to the overall integrity of the ECM.

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46
Q

What are the two main parts of chloroplasts involved in photosynthesis?

A

The thylakoid membranes (where light-dependent reactions occur) and the stroma (where the Calvin cycle takes place).

47
Q

What structure inside the mitochondria increases surface area for energy production?

A

The inner membrane is highly folded into cristae, increasing the surface area for cellular respiration

48
Q

What is a unique feature of animal cells that is not present in plant cells?

A

Animal cells may have channels between cells that allow for communication and material exchange.

49
Q

What types of proteins are synthesized in the rough ER?

A

The rough ER synthesizes membrane proteins and secretory proteins.

50
Q

Does the number of chromosomes vary between species?

A

Yes, the number of chromosomes varies between species. For example, humans have 46 chromosomes.

51
Q

Where are ribosomes partially assembled?

A

Ribosomes are partially assembled in the nucleolus.

52
Q

What is the function of the nucleolus inside the nucleus?

A

The nucleolus is where ribosome precursors are assembled, which include rRNA and ribosomal proteins.

53
Q

What is the cis face and trans face of the Golgi apparatus?

A

The cis face faces the ER and receives materials from it, while the trans face faces away from the ER and ships out processed products.

54
Q

Can lysosomes digest foreign particles?

A

Yes, lysosomes can digest foreign particles or invading microorganisms, contributing to the cell’s immune defense

55
Q

What is the function of phospholipids in the plasma membrane?

A

Phospholipids form the phospholipid bilayer, creating a barrier with hydrophilic heads facing outward and hydrophobic tails facing inward to separate the cell’s internal and external environments.

56
Q

What types of chemical modifications occur in the Golgi apparatus?

A

The Golgi adds carbohydrate chains to proteins and lipids, forming glycoproteins and glycolipids.

57
Q

What is the role of integrins in the ECM?

A

Integrins are receptor proteins in the plasma membrane that bind to ECM proteins and help mediate communication between the ECM and the cytoskeleton.

58
Q

What byproducts are produced in mitochondria during cellular respiration?

A

The byproducts of cellular respiration in mitochondria are carbon dioxide and water.

59
Q

What forms the chromatin in the nucleus?

A

Long DNA molecules wrapped around proteins, specifically histones, form chromatin.

60
Q

What is the difference between rough ER and smooth ER?

A

Rough ER has ribosomes and synthesizes proteins, while smooth ER lacks ribosomes and is involved in lipid synthesis and detoxification.

61
Q

What are food vacuoles in the context of lysosome function?

A

Food vacuoles are tiny cytoplasmic sacs that engulf nutrients for digestion by lysosomes.

62
Q

What do mitochondria do to produce energy?

A

Mitochondria perform cellular respiration, converting glucose and oxygen into ATP.

63
Q

Name two key membranous organelles in eukaryotic cells.

A

Mitochondria (energy production) and Golgi Apparatus (modifies and packages proteins).

64
Q

What is the main role of the Golgi apparatus in the cell?

A

The Golgi apparatus receives, refines, stores, and distributes chemical products of the cell.

65
Q

Where are chloroplasts mainly found in plants?

A

Chloroplasts are primarily found in the leaves and other green or colored organs of plants and in algae

66
Q

What are the main functions of the extracellular matrix (ECM)?

A

The ECM provides structural support, helps adhere cells together, and allows cell communication to regulate processes like growth and differentiation.

67
Q

What is a key modification that occurs in the rough ER for some proteins?

A

Some proteins undergo glycosylation, where carbohydrates are added to form glycoproteins.

68
Q

Which organisms are made up of eukaryotic cells?

A

Protists, plants, fungi, and animals.

69
Q

What happens when a food vacuole fuses with a lysosome?

A

The enzymes in the lysosome break down the food inside the vacuole, allowing digestion to occur.

70
Q

How do vacuoles in plant cells differ from those in animal cells?

A

Plant cells have large vacuoles that store materials, waste, and help maintain internal pressure for structural support. Animal cells have smaller vacuoles.

71
Q

In which types of cells are mitochondria found?

A

Mitochondria are found in almost all eukaryotic cells (both plant and animal cells).

72
Q

What is the function of the nucleus in eukaryotic cells?

A

The nucleus contains the cell’s DNA and controls cellular activities.

73
Q

How do lysosomes contribute to cell nutrition?

A

Lysosomes digest food engulfed in food vacuoles, providing essential nutrients to the cell after breaking down large molecules.

74
Q

What is the resolution of a Transmission Electron Microscope (TEM)?

A

TEM provides very high resolution (2 to 4 Å), allowing for observation at a molecular level.

75
Q

What is the function of chloroplasts in plant cells?

A

Chloroplasts convert light energy into chemical energy through photosynthesis to produce food for the plant.

76
Q

What do intermediate filaments provide for the cell?

A

Intermediate filaments provide structural support and help maintain cell shape, but they do not participate in cell movement.

77
Q

Which organisms are made up of prokaryotic cells?

A

Bacteria and Archaea.

78
Q

What is the function of proteoglycans in the ECM?

A

Proteoglycans help retain water in the ECM and provide structural support to tissues, aiding in their flexibility and cushioning.

79
Q

What happens to the small molecules produced from digestion inside lysosomes?

A

The small molecules are released from the lysosome and are used to nourish the cell.

80
Q

What type of lipids does the smooth ER produce?

A

The smooth ER produces steroids and phospholipids, essential for cell membranes.

81
Q

What are the two key characteristics of the plasma membrane described in the fluid mosaic model?

A

The two key characteristics are fluidity (molecules can move freely) and mosaic (the membrane has a variety of proteins).

82
Q

What are the digestive enzymes in lysosomes used for?

A

The enzymes break down macromolecules such as proteins, fats, polysaccharides, nucleic acids, and damaged organelles.

83
Q

What organelle is formed in the Golgi apparatus?

A

The Golgi apparatus is responsible for the formation of lysosomes, which digest cellular material.

84
Q

What is the function of ribosomes?

A

Ribosomes are the protein-synthesizing machines of the cell.

85
Q

What is the primary function of lysosomes?

A

Lysosomes break down large molecules (proteins, fats, polysaccharides, nucleic acids) and damaged organelles in the cell.

86
Q

Does the amount of DNA vary across species?

A

Yes, the amount of DNA in a cell depends on the species and can differ significantly.

87
Q

How does a Light Microscope (LM) achieve magnification?

A

Magnification is achieved by the objective lens and ocular (eyepiece) lenses, with illumination provided by light.

88
Q

What is the primary composition of the plasma membrane?

A

The plasma membrane is composed of lipids (mainly phospholipids) and proteins.

89
Q

What theory explains the origin of mitochondria and chloroplasts?

A

The endosymbiont theory suggests that mitochondria and chloroplasts were once free-living prokaryotes that became incorporated into eukaryotic cells.

90
Q

What are the main functions of the smooth ER?

A

The smooth ER is involved in lipid synthesis, detoxification of drugs, calcium storage, and carbohydrate metabolism.

91
Q

What is the role of the plasma membrane?

A

The plasma membrane controls the entry and exit of materials and helps maintain communication with the environment.

92
Q

What are microfilaments made of and what is their role in muscle contraction?

A

Microfilaments are made of actin proteins and play a key role in muscle contraction by interacting with myosin

93
Q

What role does the Golgi apparatus play in the cell?

A

The Golgi apparatus modifies, sorts, and packages proteins and lipids for transport.

94
Q

What types of molecules do peroxisomes break down?

A

Peroxisomes break down fatty acids, amino acids, and toxins (e.g., alcohol, drugs).

95
Q

What does the cytoskeleton in eukaryotic cells do?

A

Provides structure and helps with cellular movement using microtubules, intermediate filaments, and microfilaments.

96
Q

What is the primary function of mitochondria?

A

Mitochondria convert the energy in food molecules into ATP through cellular respiration.

97
Q

What role do the pores in the nuclear envelope play?

A

They regulate the selective movement of materials between the nucleus and the cytoplasm.

98
Q

What is the composition of eukaryotic ribosomes?

A

Eukaryotic ribosomes are made of 4 rRNA molecules and about 80 ribosomal proteins.

99
Q

What are lysosomes and their function?

A

Lysosomes contain digestive enzymes that break down waste materials, cellular debris, and foreign substances.

100
Q

What is the purpose of the cell wall in plant cells?

A

The cell wall provides protection, structural support, and helps prevent the plant cell from absorbing too much water. It is made of cellulose.

101
Q

Which cytoskeletal component is involved in cytokinesis?

A

Microfilaments (actin filaments) are involved in cytokinesis, helping to pinch the cell membrane during cell division.

102
Q

What does each chromatin fiber constitute?

A

Each long chromatin fiber constitutes one chromosome.

103
Q

Where are ribosomes completely assembled?

A

Ribosomes are completely assembled in the cytoplasm

104
Q

What are the main functions of the cytoskeleton?

A

The cytoskeleton provides mechanical support, maintains cell shape, and facilitates movement.

105
Q

What does DNA do to direct protein production?

A

DNA transfers its coded information into messenger RNA (mRNA), which directs protein production.

106
Q

What is the main difference between TEM and SEM?

A

TEM passes an electron beam through the specimen for internal details, while SEM scans the surface of the specimen, providing a 3D image with great depth of focus.

107
Q

What types of substances can vacuoles store in plant cells?

A

Vacuoles can store organic nutrients, waste, pigments (e.g., autumn colors), and poisons (e.g., nicotine).

108
Q

What are the two main energy-producing organelles in cells?

A

Chloroplasts (in plant cells) and mitochondria.

109
Q

What is the role of vacuoles in plant cell structure?

A

Vacuoles absorb water to develop hydrostatic pressure, helping to keep the plant turgid and maintain its shape.

110
Q

What is the matrix of a mitochondrion?

A

The matrix is the innermost part of the mitochondrion, where the Citric Acid Cycle occurs and contains enzymes for cellular respiration.

111
Q

Why does a Transmission Electron Microscope (TEM) operate under a vacuum?

A

The vacuum prevents electrons from being absorbed or deflected by air, ensuring clear imaging.

112
Q

What is meant by the fluid mosaic model of the plasma membrane?

A

The fluid mosaic model describes the membrane as a flexible, dynamic structure with lipids and proteins that can move fluidly, creating a “mosaic” pattern of various components.

113
Q

Which type of cells contain lysosomes?

A

Animal cells contain lysosomes, while they are usually absent in plant cells.

114
Q

Why do mitochondria have their own DNA?

A

Mitochondria have their own DNA because of their evolutionary history as free-living prokaryotes, as suggested by the endosymbiont theory.