Organisation Flashcards
Define enzyme
a biological catalyst that controls the rate of reactions in our body
Define catalyst
a substance which increases the speed of a reaction without being used up
Why can enzymes only catalyse one specific reaction?
because their active site has a unique shape that matches with a substrate - ‘lock and key’
What two factors can cause an enzyme to denature?
- temperatures that are too high
- a pH that is either too high or low
What happens when an enzyme denatures?
the shape of the active site changes
- Name 3 digestive enzymes
- What molecules they break down
- what the molecules convert into
- Protease : Proteins -> Amino acids
- Lipase : Lipids -> glycerol + fatty acids
- Amylase : starch -> maltose
- (Carbohydrase : Carbohydrates -> simple sugars)
Why is it important that food molecules are broken down during digestion?
So they are soluble and can be absorbed into the blood stream
Where is amylase made?
- Pancreas
- Small intestine
- Salivary glands
Where are proteases made?
- Pancreas
- Small intestine
- Stomach (where it’s called pepsin)
Where are lipases made?
- Pancreas
- Small intestine
Where is bile produced, stored and released?
- Produced - liver
- Stored - gall bladder
- Released - small intestine
Why does bile neutralise acids?
Because it’s an alkaline. The enzymes work best in these alkaline conditions.
Why does bile emulsify fats?
Because the fats break into tiny droplets they have a bigger surface area for enzymes to work on - making digestion faster.
Why does the stomach produce hydrochloric acid?
to kill bacteria,
to give the right pH for protease to work
How do you prepare a food sample?
- Break it up using a pestle and mortar
- Transfer to beaker, add some distilled water
- Stir with glass rod to dissolve some of the food
- Filter the solution using a funnel lined with filter paper to remove solid bits of food
- …
Which solution do you use for each food test?
Sugars - benedict’s solution
Starch - iodine solution
Proteins - biuret solution
Lipids - ethanol
What colour’s shown when sugar is present?
from blue to green, yellow, brick-red
in ascending order of how much is present ->
What colour’s shown when starch is present?
from browny-orange to blue-black
What colour’s shown when protein is present?
from blue to purple
What colour’s shown when lipids are present?
cloudy-white
What food test requires a water bath at 75C ?
test for sugars
using benedict’s solution
What food test should not be filtered when preparing a food sample?
test for lipids
because it sticks to the filter paper
What should you do after adding a solution to your food samples?
gently shake the test tube
How does air travel through when breathing in?
trachea -> bronchi -> bronchioles -> alveoli