Cell biology Flashcards
What is the difference between eukaryotic and prokaryotic cells?
Eukaryotic cells are complex and have a nucleus
Prokaryotic cells are simpler and smaller
What is a eukaryote?
An organism made up of eukaryotic cells
Name all the organelles in an animal cell
there are 5
1. Nucleus
2. Cytoplasm
3. Cell membrane
4. Ribosomes
5. Mitochondria
Name all the extra organelles in a plant cell
not including those in an animal cell
1. Cell wall
2. Permanent vacuole
3. Chloroplasts
4. + those in an animal cell
What is a prokaryote and an example
It is a prokaryotic cell (single-celled organism)
Example: bacteria
Name the organelles in a bacterial cell
1. Cytoplasm
2. Cell membrane
3. Cell wall
4. Plasmids
5. Single strand of DNA
Name the 2 types of microscopes
Light microscopes
Electron Microscopes
How do electron microscopes work? What do they let us see?
They use electrons to form an image. They have much higher magnification and resolution.
They let us see smaller things in more detail (e.g. internal structure of mitochondria)
How do light microscopes work? What can they let us see?
They use light and lenses to form an image of a specimen and magnify it.
They let us see individual cells and large subcellular structures (e.g. nuclei)
What is the formula for magnification?
magnification = image size / real size
How do you prepare a slide for onion cells?
- Add a drop of water to the middle of a clean slide
- Cut up an onion + seperate its layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers
- Using the tweezers, place the epidermal tissue into the water on the slide
- Add a drop of iodine solution (a stain) which will highlight the object
- Place a cover slip on top. Try to avoid air bubbles - they’ll obstruct the view.
How do you use a light microscope to observe a slide?
- Clip the slide onto the stage
- Select the lowest-powered objective lens
- Adjust mirror and turn on the light
- Use the coarse adjustment knob to move the stage up to just below the objective lens
- Slide must not touch lens
- Look down eyepiece. Use coarse adjustment knob to move stage downwards until the image is roughly in focus
- Adjust focus with fine adjustment knob, until you get a clear image,
How do you work out the total visual magnification from a microscope?
magnification of objective lens x magnification of eyepiece = total visual magnification
Define differentiation
the process by which a cell changes to become specialised for its job
Can cells always differentiate?
most animal cells lose the ability at an early stage
most plant cells retain the ability to differentiate throughout their life
What is an undifferentiated cell called?
stem cell
Name 5/6 specialised cells and what they’re specialised for
sperm cells - reproduction
nerve cells - rapid signalling
muscle cells - contraction
root hair cell - absorbing water + minerals
phloem + xylem cells - transporting substances
How is a sperm cell specialised?
lots of mitrochondria - provides energy from glucose
flagella + streamline head - mobility + movement
enzymes - digest through egg cell membrane
nucleus - contains DNA of father
How is a nerve cell specialised?
many endings - to connect with other adjacent nerve cells
myelin sheath - insulating cover
axon - (the extension of a nerve cell along which electrical impulses travel) to cover more distance
How is a muscle cell specialised?
lots of mitochondria - to generate energy for muscle contractions
(protein filaments - to determine shape and movement)
How is a root hair cell specialised?
Small thin extension - to increase surface area or root allowing plant to absorb more water + minerals from soil
How is a xylem cell specialised?
long cells and joined end to end - to form the xylem tube
hollow - so water can flow through
How is a phloem cell specialised?
long cells and joined end to end - to form the tube
few subcellular structure - so food substances (mainly sugars) can flow through
Where is genetic material found?
In the nucleus, in the form of a chromosome
Define chromosome
coiled up strands of DNA containing large numbers of genes