ORGANISATION Flashcards
cells are…
a tissue is…
organs are….
organs are organised into …. which work together to …..
the basic building blocks of all living organisms
-a group of cells w/ similar structure + function
-aggregations/group of tissues(that work together) performing specific functions
-organ systems- form organisms
what do
glandular tissue
epithelial tissue
do
glandular tissue- makes + secretes chemicals(makes digestive juices)
epithelial tissue- covers some parts of body (outside + inside of stomach)
in the digestive system , what do these organs do-
glands
stomach
small intestine
liver
large intestine
glands-produce digestive juices
stomach -digest food
small intestine-digest food and absorbs soluble food molecules
liver-produce bile
large intestine-absorbs water from undigested food
what does muscular tissue do- how x2
-muscular tissue moves stomach walls to churn food
muscular tissue-has muscle cells which contract for movement
the muscles cells have protein fibres which change length(to allow contractions)
-muscle cells have many mitochondria- provide energy for contraction
()
an organ is a group of tissues working together- give an example
stomach- contains muscular tissue and glandular tissue
3 main nutrients in food
-why do they have to be digested
-what happens during digestion
carbs(eg starch)-protiens-lipids(fats)
-large molecules- too large to be absorbed into blood stream
-digestive enzymes break large molecules into smaller ones so they can pass through the walls of digestive system and be absorbed into bloodstream
mouth,
oesophagus,
stomach,
small intestine,
large intestine
rectum.
-liver
-pancreas
-gall bladder
1)mouth-amylase enzymes in saliva digest starch into smaller sugar molecules
2)oesophagus-food passes down to
3) stomach- enzymes begin digesting protiens
-hydrochloric acid helps enzymes
-muscular walls churns food to fluid
4) sm. intestine-
chemicals released from liver(bile) and pancreas (enzymes)
-digestion continued
-walls of sm. i release enzymes
-small food molecules absorbed into bloodstream
5)large intestine-
water absorbed into blood stream
what happens in the mouth
what happens in the oesophagus
amylase enzyme in saliva digest starch into smaller sugar molecules
food passes down to stomach
what happens in stomach
1- why
2
3- and why x2
1-muscular walls churn food into fluid - increases s.a for enzymes to digest
2-produces protease enzyme pepsin-begins digesting proteins
3-produces hydrochloric acid to -
-kill bacteria
-give right acidic ph for protease enzyme to work
what happens in sm. intestine
1
2
3
4
1)where most of the digestion happens
2)digested food absorbed into bloodstream(diffuse/activetransport)
3) protease, amalyse,lipase enzymes from pancreas in form of pancreatic juices complete digestion
(also creates some itself)
4)gets bile from gall bladder
what does bile do x3
where is bile made
where is bile stored
-neutralises acid from stomach,
-makes ph more ideal for digestive enzymes
-emulsifies fats into smaller droplets-for greater s.a for enzymes to work on
-made-liver
-stored-gall bladder
what happens once food is digested in small intestine
-how is the lining of the small intestine adapted
x3
absorbed into blood stream
-has loads of villi which increase s.a of small intestine so diffusion of digested food into the blood stream is quicker
-villi have single layer of surface cells- short diffusion pathway
-villi- good blood supply-maintains concentration gradient
-what is the material left in the sm. intestine like-why
-what happens to this material that wasn’t absorbed into blood
x3
-because of secreation from pancreas,stomach,bladder, leftover material is watery
1)material passes to large intestine-
2)excess water absorbed by large intestine- faeces left behind
3)faeces left in rectum
9 steps to the digestive system
-food chewed-salivary glands add saliva
-down oesophagus and into stomach
-fluid pushed into small intestine
-mixes w/ pancreatic juices+ bile
-broken down and digested
-small molecules absorbed from sm. intestine into blood stream
-molecules that X be absorbed go to large intestine
-water from leftover material absorbed back into body
-faeces left behind in rectum
enzymes are—-
what type of molecules are enzymes-
describe lock and key theory-3 points
biological catalysts that speed up(catalyse) chemical reactions without being changed or used up
large protien molecules- all protiens made of chains of amino acids
-The shape of the substrate is complementary/specific to the shape of the active site
-when they bond it forms an enzyme-substrate complex.
-Once bound- reaction takes place, products released
from surface of the enzyme
what can enzymes do-not about catalysing
break up large molecules or join small ones.
what do digestive enzymes do
what are the products of digestion used for
convert food into small soluble molecules that can be absorbed into bloodstream
- build new carbs, lipids/protiens- some glucose used in respiration
what are the 3 digestive enzymes, what do they break down
what do they break them into
amylase(carbohydrase)- breaks starch(carb) into glucose(simple sugars eg maltose)
Proteases break down proteins into amino acids
Lipases break down lipids/fats into fatty acids + glycerol
protease enzymes made in
-what are protiens
-what does protease enzymes do -why(dont talk about digestion)
stomach
pancreas
small intestine
long chains of amino acids
-break protiens back into amino acids which are absorbed by body cells and joined in different order to make human proteins
amalyse made in
amalyse is a ….
-what is starch made of
salivary glands, pancreas, small intestine
carbohydrase
-chain of glucose molecules(it is a type of carb)
lipase is made in….
what is a lipid molecule made of-
pancreas and small intestine
-glycerol molecule + 3 fatty acid molecules
bile is made in …… and stored in …… It is ….. to neutralise ……. ……. from the stomach. It also ……. fat to form …… ……. which increases the ……. ……… The ……. conditions and large ……. ….. increase rate of fat breakdown by ……..
made in liver
stored in gall bladder
Its alkaline
neutralise hydrochloric acid in stomach
It emulsifies fat
to form small droplets
Increases S.A
The alkaline conditions & large S.A
increase rate of fat breakdown by lipase
4 food tests-what is added, what is a +ve test
Starchy Dina
Sweet Benedict
Protien Biuret
Fatty Sudan
-Starchy Dina- add iodine solution- —-> bluey/black
-Sweet Benedict-add benedict solution.
—> brick red
-Protien Biuret- add biurets
—-> purple
-Fatty Sudan-add Sudan III stain
—->separates to 2 layer- top one red
how do u prepare a food sample
-break up food w/ mortar + pestle
-put in beaker- add distilled water
-stir w/ glass rod
-filter -funnel lined w/ filter paper
-use the watery solution
what does the benedict test for
-steps to the test x4
-a positive test would be…
-safety precautions x2
-reducing sugars
1)add 5cm3 sample to test tube
2)prep water bath @ 75*c
3)add 10 drops benedict to tube
4)put tube in bath- 5 mins
- blue —> green/yellow/brick red- depend on sugar conc.
*use test tube holder
*point tube away from u
iodine test for starch
-steps to the test x 3
-a positive test would be
1) 5cm3 sample to tube
2) few drops iodine solution- will turn browny orange
3)gently shake
-browny orange —–> bluey black
biuret test for protiens
-steps to the test x 3
-a positive test would be
1) 2cm3 sample + 2cm3 biuret solution to tube
2)will turn blue
3)gently shake
-blue—-> pink/purple
Sudan lll test for lipids
-steps to the test x 3
-a positive test would be
1) 5cm3 sample (maybe unfiltered)
2) + 3 drops Sudan lll stain solution
3) gently shake
-mixture separate to 2 layers- top layer bright red
-what happens to reaction if temp increases
why
what does -‘optimum temp’ mean
what is optimum temp of most human enzymes
what happens if temp increased above optimum-why
-as temp increases, rate increases
-particles have more kinetic energy
-more collisions per second btwn substrate + active site
-once optimum temp exceeded- rate decreases
optimum- temp where enzyme working at fastest rate- maximum frequency of successful collisions btwn substrate and active site
-37*c
-activity of enzyme rapidly decreases.
-too hot- bonds holding enzyme break- shape of active site changes-
-enzyme denatured
-enzyme can’t catalyse reaction
-what happens to reaction if ph made more acidic/ alkali then the optimum- why
what does -‘optimum ph’ mean
generally, optimum enzyme ph is-
-Enzymes released from pancreas into sm. intestine work best at ….
-Enzymes in stomach work best at…
enzyme activity drops to 0
-active site denatures if conditions too acidic/alkaline
-ph when enzyme works at fastest rate-rate of reaction highest
-7-neutral- (enzymes have specific optimum ph’s)
-alkaline ph
-acidic ph
is the damage done to an enzyme when it denatures permanent or reversible
The presence of starch is a sign that…
-how
permanent
.
.
that photosynthesis has occured
-product of photosynthesis is glucose
-plants store glucose as starch
RP effect of Ph on amylase
Step 1
Place drop of iodine solution into each well of spotting tile
Step 2
Take 3 test tubes - label each one
Step 3
1st test tube = add 2cm cubed of starch solution
2nd test tube = add 2cm cubed of amylase solution
3rd test tube = add 2cm cubed of PH5 buffer solution
Step 4
Place all 3 test tubes in water bath at 30 degrees-leave for 10 mins so they reach right temp
Step 5
Combine 3 solutions into one test tube + Mix w/ stirring rod
Step 7
Return test tubes to water bath- start stop watch
Step 8
After 30 seconds use the dropping pipette to transfer one drop of solution to a well in the spotting tile that contains iodine
what does buffer solution do
what will happen in the spotting tiles-
-how long do we continue to add solution to the tiles.
-why do we stop
-what do we do once iodine doesn’t change to bluey black
Buffer solution - used to control PH
The iodine turn blue-black as starch is present
-Take a sample every 30 seconds and continue until iodine remains orange
When iodine remains orange - tells us that starch is no longer present and the reaction has been completed
stop timer-
We record the time for this in our results
Repeat whole experiment several times using different PH Buffers
eg PH 6, PH 7, PH 8
Rp effect of Ph on enzyme activity
-what are the problems with the experiment-how would you address it x4
What are the control variables
-only taking samples every 30 secs, only have approximate time.
-take sample every 10 secs
-not always obvious when iodine doesn’t go bluey black- hard to see when reaction complete -
-ask few ppl to look at tile + decide when reaction complete
-use a ph meter to measure ph more accurately
-could use an electric heater to control temp - more accurate than a normal water bath
-concentration/ volume of amylase solution
how to calculate rate of reaction x2
what’s the 2 units for rate
1000/time
s to power -1
OR
amount of product formed/time
cm 3 / s to power -1
how is the small intestine adapted to absorbing products of digestion
x5
-very long-large s.a for absorbtion of products of digestion
-interior covered in villi- increase s.a
-villi have microvilli- further increase s.a
-villi have good blood supply-blood stream rapidly removes products of digestion- increases conc. gradient
-villi have thin membrane- short diffusion path
describe track of air through the different organs and parts of your chest- 5 points
(thorax is the top part of the bod, separated from lower bod by diaphragm) (Lungs protected by ribcage + surrounded by pleural membranes)
-Air goes through trachea
-trachea splits into 2 bronchi
-each bronchi goes to each lung
-bronchi then split in to bronchioles
-bronchioles end in sacs called alveoli
-feature of trachea
-what happens at alveoli
rings of cartilage stop trachea collapsing when inhaling
- gases diffuse in and out of blood- sites of gas exchange
how does breathing affect rate of gas diffusion in alveoli
increases rate- brings fresh O2 into alveoli, takes away carbon- dioxide- makes concentration gradient high
process of alveoli in gas exchange- 3 steps
-blood passing through capillaries, next to alveoli contains lots of CO2- little O2 - has just returned from rest of body
-oxygen diffuses out alveolus into blood.
-Carbon dioxide diffuses out blood to alveolus to be breathed out
-process of gas exchange btwn blood and body cells - 3 steps
-oxygenated blood reaches cells,o2 diffuses into cells
-CO2 diffuses out cells into blood and carried back to lungs
-then diffuses into alveoli to be breathed out
how can you calculate breathing rate per min
-bob takes 91 breaths in 7 mins- calculate average rate in breaths per minute
no. of breath/ no. of minutes
91/7 = 13
13 breaths per minute.
describe the single circulatory system in fish
-4 points
-deoxygenated blood pumped from heart to gills
-collects oxygen
-oxygenated blood passes straight to organs
-O2 diffuses into body cells
-blood returns to heart
what is a problem w/ single circulatory system in fish
blood loses alot pressure as it passes to gills bfore organs- so passes to organs slowly- so can’t deliver alot of oxygen
describe the double circulatory system in humans
6 points
3 for right ventricle
3 for left ventricle
-right ventricle pumps deoxygenated blood to lungs
- it takes in o2
- oxygenated blood returns to heart
-left ventricle pumps oxygenated blood around body.
-oxygen diffuses from blood into body cells.
-deoxygenated blood returns to heart to be pumped to lungs again
what are heart walls mainly made of-
what are the four chambers of the heart as though you were looking at a heart
-where are the valves- what do they do-
muscle tissue
right atrium ||||| left atrium
right ventricle ||||| left ventricle
-stop backflow of blood in the heart (when atria contract)- they separate the atria from ventricles
what are the 4 main blood vessels entering and leaving heart
-what side are they on
which side of heart deals w/ O2 blood
pulmonary artery-R-upper
vena cava- R-lower
aorta-L-upper
pulmonary vein-L-lower
-left side- the side that is on your right when u look at a pic- deals with oxygenated blood
what do these do:
vena cava
pulmonary artery
pulmonary vein
aorta
-vena cava- brings XO2 blood from body to heart
-pulmonary artery- carries XO2 blood from heart to lungs
-pulmonary vein- carries O2 blood from lungs to heart
-Aorta- pumps O2 blood from heart to body
describe the pattern of blood flow through the heart- 3 points
-blood enters right atrium from vena cava
and left atrium from pulmonary vein
-atria contract, blood forced into ventricles
-ventricles contract - blood forced out of heart
through pulmonary artery (to lungs)
through aorta (to body)
-which side has a thicker muscular wall
-why
left side- (side on your right if u look at pic)
bcse left ventricle pumps blood around body- so needs greater force
what are coronary arteries
what do they do-
how is this helpful
-arteries that branch out from aorta + spread into heart muscle
-provide oxygen to muscle cells of heart.
-the oxygen is used in respiration to provide energy for contraction
-how is natural resting heart rate controlled
-where is it
-what do they do
by group of cells that act as a pacemaker
-right atrium
(produce small electrical impulses that spread through the muscular walls of the heart, causing them to contract)
what are artificial pacemakers
electrical devices used to correct irregularities in heart rate
—-implanted under skin- has wire going to Heart-produces electric current to keep heart beating regularly—-
what do these blood vessels do-
Arteries
capillaries
veins do
carry high press blood Away from heart
-exchange nutrients + oxygen w/ tissues
- carry blood to heart
-why do arteries need to be strong and elastic
features of arteries
x3
because blood is pumped from heart at high pressure
-walls have thick muscular tissue- to withstand high pressure blood from heart
-walls contain elastic fibres- stretch when surge of blood passes through + recoil between surges- keeps blood moving
-therefore walls are thick compared to lumen
what do capillaries do-
features of capillaries x4
-blood passes through capillaries, glucose/food/o2 ect diffuse into cells and co2 out from cells to blood
(exchange substances w/ cell)
-permeable walls- substances can diffuse in/out
-very thin walls-increase rate of diffusion-shorter diffusion distance
-small lumen
-very tiny