CELL BIOLOGY CGP Flashcards
what parts do most animal cells have-
what extra parts do plants have
what extra part do plant and algal cells also have-why
nucleus
mitrochondria
ribosomes
cytoplasm
cell membrane
cell wall
-chloroplasts
-permanent vacuole
-cellulose cell wall to strengthen cell + chloroplasts.
what do all eukaryotic cells have- x3what cells are these
compare prokaryotic cell to eukaryotic cell-
What parts are in eukaryotic cell x5
cell membrane, cytoplasm. genetic material enclosed in nucleus- Plant and Animal
pro are smaller
pro dont have mitrochondria
pro circular dna strand floats freely in cytoplasm- no nuclues
pro have plasmids- small rings of DNA
both have cytoplasm,
both have cell membrane,
cell memrane, cytoplasm. nucleus, ribosomes, mitrochondria
describe
nucleus
mitrochondria
cytoplasm
cell membrane
ribosomes
nucleus-has genetic material that controls cell
mitrochondria- site of aerobic respiration, provide energy for cell
cytoplasm-gel like substance- most chemical reactions happen- has enzymes to control the reactions
cell membrane-holds cell tgether, controls what goes IN + OUT
ribosomes-site of protien synthesis
describe
cell wall
permanent vacuole
chloroplasts
cell wall- supports, strengthens cell
permanent vacuole- contains cell sap- sugar + salt solution to keep cell turgid
chloroplasts-site of photosynthesis- chlorophyll absorb light.
do bacteria cells have mitrochondria
do bacteria cells have ribosomes
what are the parts of a bacteria cell x6
no
yes
cell wall
cell membrane
cytoplasm
circular strand
plasmid
ribosomes
how do light microscopes work
how do electron ones work
use light and lenses to form an image of a specimen and magnify it
-use electrons to form an image
measurement names in descending order and symbols
what would you times it by to get number in metre
metre m
centimetre cm 10^-2
millimetre mm 10^-3
micrometre μm 10^-6
nanometre nm 10^-9
pros of electron microscopes - x2
can we see mitrochondria and chloroplasts through electron microscopes
can we see ribosomes and plasmids through electron microscopes
higher magnification and resolving
power than a light microscope.
allow biologists to see and understand more subcellular structures
yes can see their internal structure
yes- tinier than mitrochondria and plasmids
what is resolution
what is magnification
units for magnification
unit for image size is
unit for real size is
how do you convert between these units
ability to distinguish btwn 2 points - higher resolution = sharper image
-how big an object looks
no unit
mm
µm
from µm to mm, divide by 1000,
mm to µm, X by 1000
ribosomes are very small. Can they be seen with an electron microscope, a light microscope, or both
-whats smaller, mitochondria or ribosomes
electron
ribosomes
difference bwn plant and animal cell
plants have a regular shape
(animal cells can change their shape easily)
plant cells packed with chloroplasts, vacuole, cell wall
how has microscopy techniques developed over time
as tech and knowledge has improved
how have electron microscopes increased understanding of subcellular structures
3 points
uses electrons to form an image with much higher magnification AND resolution-
see smaller things in more detail-internal structure of mitochondria + chloroplasts
-see tinier things -ribosomes + plasmids
how do you prepare a slide
6 steps-
-add drop of water to middle of clean slide
-cut up onion, separate into layers. Use tweezers, pull of epidermal tissue from bottom of a layer
-use tweezers- put epidermal tissue into water on slide
-add drop of iodine solution- a stain
-put cover slip on top
-stand slip up, next to water droplet, tilt + lower it so it covers specimen.
important thing to remember when preparing slide
what do stains do
what does iodine stain do
try not get air bubbles when putting cover slip on- obstructs view of specimen.
-hold slide/ slip by edges, dont get fingerprints on it - obscure image
highlight objects in a cell by adding colour- make subcellular structures easier to see.
stains the starch in onion cells
what are parts of microscope slide- 6 parts
eye piece
high/low power objective lenses
stage
coarse adjustment knob
fine adjustment knob
light/lamp
what do some light microscopes have instead of a light- what does this do
a mirror- the mirror reflects light through microscope slide
how many objective lenses do most microscopes have- give example of these
what does the eyepiece have
3
4x 10x 40x
the eyepiece lens- 10x magnification
how to use light microscope to look at slide- 6 steps
What would you do if u need greater magnification
-clip prepared slide onto stage
-select lowest powered objective lens
-position lens so almost touches slide
-to do this- use coarse adjustment knob to move stage just below objective lenses
-look down eyepiece. Use coarse adjustment know to move stage downwards till image in rough focus
-adjust focus w/ fine adjustment knob till you get clear image
—swap to higher powered objective lens+ refocus
what is important to remember when adjusting level of objective lens or the power of objective lens
look from the side so u see how close the slide + lens are so u dont damage slide
things to remember when drawing observations x7
p
1/2
c
c
p
t
ll
- use sharp pencil
-should take up minimum 1/2 space available
-draw with clear, unbroken lines
-dont colour/shade
-subcellular structures drawn in proportion
-add title of what you were observing and magnification used
-label important features eg nucleus w/ un-crossed lines
how can you find size of cell w/out using magnification equation
Try find a row with whole cells along diameter
Count how many cells eg 4
get a ruler and use microscope to measure diameter of field of view eg 2 mm
turn mm to um 2mm= 2000 um
2000um divide by 4 cells = 500um per cell
parts of baceria cell- x6
cell membrane
cell wall
circular strand of dna
plasmids
cytoplasm
ribosomes
what is cell differentiation
What happens as an organism develops
when do most animal and when do most plant cells specialise
process by which cell changes to become specialised for its job
-cells differentiate- to form different types of cells
-animal cells- at early stage
-plant cells- retain ability to differentiate throughout life
why is differentiation important
-in mature animals- cell division is restricted…….
what happens to a cell as it differentiates-
-allows cell to carry out specific function
-restricted to repairing and replacing cells
- develops different sub-cellular structures to enable it to carry out certain function
***when using an optical/light microscope on an plant cell, what structures can be seen
cytoplasm
nucleus
cell wall
vacuole
chloroplasts
cell membrane
when is a cell said to be specialised
what are stem cells
when it has differentiated and has different subcellular structures to carry out it specific function
UNdifferentiated cells
3 specialised animal cells
3 specialised plant cells
sperm, nerve, muscle cells
root hair, xylem, phloem cell
SPERM CELL
function-
how are they specialised x4
to join with the egg cell- so genetic information of male and female can combine during fertilisation
1-nucleus has 1/2 the genetic info of a normal adult cell
2-long tail, streamlined head-help swim to egg
3-packed with mitochondria, provide energy to swim
4-enzymes (in head) digest through egg cell membrane
NERVE CELL
function-
how are they specialised x4
-carry electrical impulses around body.
1-long axon-carries electrical impulses from+to part of body
2-myelin sheath insulates axon- speed up transmission of nerve impulses
3-end of axon has synapses-allow impulse to pass to another nerve cell
4-cell body has dendrites-increase s.a so other nerve cells can more easily connect
MUSCLE CELLS
function-
how are they specialised x2
contract for movement
1- contain protein fibres which can change length(when cell contracts, fibres shorten= length of cell decreases)
2- contain lots of mitochondria-provide energy for contraction
3–long so they have space to contract-
ROOT HAIR CELL
function
where are they-
how are they specialised x3
-absorb water + minerals
- on surface of plant roots
1-large s.a -have long projection-the root hairs stick out into soil
(2-X contain chloroplasts- underground)
2- thin walls
3-lots of mitrochondria
XYLEM CELL
function
where are they found
how are they specialisedx3
-form long tubes- carry water + minerals from roots to leaves
-in plant stem
1-thick walls contain lignin-provides support for plant
2-end walls btwn cell broken down-form long tube for water,minerals to flow
3-X internal structures(nucleus, cytoplasm, vacuole,chloroplasts-) easier for water,minerals to flow
PHLOEM CELL
function
where is it found
how are they specialised x3
-form tubes -carry dissolved sugars up+down plant
1-phloem vessel cell has X nucleus, limited cytoplasm
2-end walls of vessel cells have pores-sieve plates
BOTH ALLOW DISSOLVED SUGARS TO MOVE THROUGH CELL
3-companion cell connected by pores- have mitochondria to provide energy to phloem vessel cell
define stem cell
where are they found-
undifferentiated capable of giving rise to many more cells of same type, and from which certain other cells can arise from differentiation
-early human embryos
-adult bone marrow
-Meristem tissue in plants
what can stem cells in adult bone marrow do
what can stem cells from human embryos be used for
what can Meristem tissue in plants do
differentiate to form many types of cells inc. blood cells
-cloned and made to differentiate into most different types of human cells
-differentiate into any type of plant cell, throughout life of plant
what are stem cells from embryos and bone marrow used in- how
-medicine and research
-stem cells grown in lab, to produce clones and made to differentiate (depending on the instructions given) into specialised cells which are used
eg of how does medicine currently use adult stem cells
stem cells from bonemarrow of healthy person can replace faulty blood cells in person who receives them
how is an adult made- 4 steps
-sperm cell fertilises egg cell
-fertilised egg undergoes mitosis- forms ball of cells-embryo
-cell in embryo continue to undergo mitosis and differentiate to specialised cells
-these cell eventually form adult
how would someone with bone marrow cancer be treated using stem cells - 3 points
-existing bone marrow destroyed w/ radiation
-patient recieves transplant of bone marrow from healthy donor
-stem cells in bone marrow divide and form new bone marrow + differentiate to form blood cells
problems with bone marrow stem cell transplants x2
donor must be compatible w/ patient or the stem cells may be rejected or wbcells produced by donated bone marrow could attack patient’s body
-risk of viruses passed from donor to patient
how could stem cells help diabetes/ paralysis-3 steps
-embryonic stem cells could replace faults cells
-could make insulin producing cells for ppl w/ diabetes
-or nerve cells for paralysed ppl