Organisation Flashcards
How are muscle cells specialised x 2
they can contract and contain special protien fibres which change their length
full of mitochondria which provide energy for contraction
what are cells
Cells are the basic building blocks of all living organisms.
what are tissues
A tissue is a group of cells with a similar structure and function
what are organs
Organs are aggregations (groups) of tissues that work together performing specific functions
what are organs
Organs are aggregations (groups) of tissues that work together performing specific functions
example of an organ
stomach, contains muscle tissue and glandular tissue which release enzymes
what does the stomach have that helps it carry out its function
contains muscle tissue and glandular tissue which release enzymes
what is an organ system
Organs are organised into organ systems, which work together to form organisms.
what three main nutrients do food contain
carbohydrates eg starch
protiens
lipids eg fat
why do the three main nutrients in food have to be digested
they are large molecules, too large to be absorbed into the bloodstream
what happens during digestion
large food molecules are broken down into small molecules by enzymes. Small molecules absorbed into bloodstream.
what happens in the mouth during digestion
food is chewed. enzymes in the saliva begin to digest the starch into smaller sugar molecules.
where does food travel to from the mouth
oesophagous
food travels from the oesophagous to the stomach. what happens in the stomach
in the stomach, enzymes begin the digestion of protiens
what does the stomach contain that helps digestion and how
hydrochloric acid which helps the enzymes digest protiens
how is the food turned into a fluid. Why is this beneficial
the churning action of stomach muscles turns food into fluid
increases s.a for enzymes to digest
where does the fluid go from the stomach
stomach to small intestine
chemicals are released into the small intestine from……
liver and pancreas
what does the pancreas release
pancreas releases enzymes which continue digestion of starch and protiens
and start digestion of lipids
Bile is made in the …… and stored in the ……..
Bile is made in the liver and stored in the gall bladder.
what does bile do
bile neutralises hydrochloric acid from the stomach
what does bile do to fat
bile emulsifies fat
to form small droplets which increases the surface area.
how does the bile increase fat breakdown
The alkaline conditions and large surface area increase the rate of fat (lipid) breakdown by lipase
what do the walls of the small intestines release
walls of small intestine release enzymes to continue breakdown of protien and lipids
what happens in the small intestines
in small intestine, small food molecules produced by digestion are absorbed into the bloodstream
by diffusion or active transport
where does food go from small intestine
large intestine
what happens in large intestine
in large intestine, water is absorbed into the bloodstream
what happens after the large intestine
faeces released rom body
what happens in the digestive system
large food molecules are digested into smaller molecules and then the products of digestion are absorbed into the bloodstream
how are the products of digestion used
used to build new carbohydrates, lipids and protiens.
state the role of enzymes
Catalyse chemical reactions
what are enzymes and what do they have on their surface
large protien molecules. have a groove on the surface called an active site
what happens at the active site
substrate attaches at active site and is broken down into products
enzymes are …… , the subtrate must fit …….. into the active site, This is called the …. and……. theory
enzymes are specific, the subtrate must fit perfectly into the active site, This is called the LOCK and KEY theory
what enzyme breaks down protiens
proteases
what enzyme breaks down lipids
lipases
what enzyme breaks down starch
amalyse
amalyse breaks down …… into…..
amalyse breaks down starch into glucose
proteas breaks down ……. into……
proteas breaks down protiens into amino acids
lipase breaks down …… into …… & …….
lipase breaks down lipids into fatty acid and glycerol
3 places amalyse is found
salivary glands
pancreas
sm. intestine
3 places proteas is found
stomach
pancreas
sm.intestine
what enzyme is found in only two places, and where
lipase is found in:
pancreas
sm.intestine
what enzymes are found in the pancreas and sm. intestine
lipase
proteas
amalyse
protiens are long chains of……. called ……. ….
protiens are long chains of chemicals called amino acids
what does proteas do to protien
proteas convert the protien back into amino acids which are absorbed into bloodstream
how are human protiens made
amino acids are absorbed by body cells and joined togethe rin a different order
what is starch and what does it consist of
starch is a carbohydrate
consists of a chain of glucose molecules
what are carbohydrates broken down by
carbohydrases
Carbohydrases break down carbohydrates to ……
simple sugars
Amylase is a ………which breaks down starch
carbohydrase ( an enyme)
lipid molecule consists of
a ……. molecule attached to ….. molecules of …. …..
lipid molecule consists of
a glycerol molecule attached to three molecules of fatty acids
what does lipase do..
it splits apart the glycerol molecule and three molecules of fatty acids
what happens to enzymes as we increase the temperature
as temp increases, the activity of the enzyme increases (reaction gets faster)
why does an increase in temp cause a faster reaction
the enzyme and substrate move faster so there are MORE COLLISIONS per second btwn them
explain the optimum temp for an enzyme
when the enzyme is working at the fastest possible rate
maximum frequency of successful collisions between substrate and active site
what is optimum temp for most enzymes
37 degrees ( human body temp)
what will we see on a graph when temperature is increase past the optimum
the enzyme activity rapidly decreases to 0
why does an enzyme (active site) denature from too high temps. (3 points)
the enzyme molecule vibrates
the shape of the active site changes
substrate no longer fits into active site
what cant happen when enzyme denatures
enzyme can no longer catalyse the reaction
describe the graph for:
effect of temp on activity of enzymes
rises diagonally to a point, then curves and decreases rapidly to 0
a .
c . .
t . .
i . .
v . .
i . .
t . .
y . .
temperature
what happens to the enzyme activity when the ph is more acidic or alkaline then the optimum
enzyme activity drops to 0 when ph is more acidic or alkaline
the active site…….. if the conditions are too ….. or too …….
active site denatures if conditions are too acidic or too alkaline.
fill in the gaps
Each …… has a …….. optimum ..
each enzyme has a specific optimum ph
what PH does a protease enzyme work best at.
acidic ph
where are protease enzymes found
in the stomach
what ph does lipase work best at
alkaline ph
lipase is released from the ……….. into the …… …….
lipase is released from the pancreas into the small intestine
describe a graph for:
effect of ph on enzymes activity
a .
c . .
t . .
i . .
v . .
i . .
t . .
y . .
PH
what solution do you use to test for starch
and what colour does a positive test turn
Starchy Dina
bluey black
use iodine to test for starch
turns bluey black
what solution do you use to test for a reducing sugar, ( glucose )
and what colour does a positive test turn
Sweet Benedict
brick red -only reducing sugars
use Benedict’s solution to test for glucose
when testing for reducing sugars, what colour will a high/ low amount turn
green/yellow if the amount is low
red if it is high.
what solution do you use to test for protein
what colour does a positive test turn
Protien Biuret
purple
add protien solution to test for protien
turn purple/lilac if protien is present
what are the first 5 steps for food tests
1.grind food sample with distilled water
2.use mortar and pestle to make a paste
3.put paste in Beaker add more distilled
4.stir so chemicals in food dissolve
5.filter solution
Rhyme to remember first 5 steps of food tests
Water
mortar
paste in beaker
stir
filter
describe test for starch
add 2cm cubed of food solution into test tube
and orange iodine solution
turns blue-black starch present
stays orange starch not present
describe the test for sugars
add 2cm cubed of food solution into test tube
add benedict solution - blue (10 drops)
Heat for 2 mins in water bath
@boiling point
colour change if sugars are present
what do different colours tell us in the test for sugar
Green- small amount of sugar
Yellow- more sugar present
Brick red- alot of sugar present
benedict test only works for …….. sugars. it will not work for ….. ……. sugars eg ……
benedict test only works for reducing sugars
it will not work for non reducing sugars eg sucrose
describe test for protien
add 2 cm cubed of food solution
then add 2 cm cubed of biuret solution (blue)
protien present:
colour change from blue to purple/ lilac
describe the test for lipids fats
add 2 cm cubed of food solution (unfiltered) to test tube
add few drops of d.water and ethanol
shake shake shake (gently;)
turns cloudy milky if lipids are present
what is the emulsion test
the test for lipids
TRUE / FALSE
we filter the solution when testing for lipids.
why/why not
FALSE
we don’t filter the solution when testing for lipids
as lipid molecules can stick to filter paper
what do you use to test for lipids
what colour does a positive test turn
Fatty Ethanol
cloudy, milky
add ethanol ( and water )
cloudy milky emulsion formed if lipid present
why do you need to take caution with ethanol
Ethanol is highly flammable
Make sure no naked flames are present
describe RP effect of ph on enzymes
Step 1
Place one drop of iodine solution into each well of a spotting tile
Step 2
Take 3 test tubes - label each one
Step 3
1st test tube = add 2cm cubed of starch solution
2nd test tube = add 2cm cubed of amylase solution
3rd test tube = add 2cm cubed of PH5 buffer solution
Buffer solution - used to control PH
Step 4
Place all 3 test tubes in water bath at 30 degrees
Step 5
Leave them for 10 minutes - to allow solutions to reach right temperature
Step 6
Combine 3 solutions into one test tube
Mix with a stirring rod
Step 7
Return test tubes to water bath and start stop watch
Step 8
After 30 seconds use the stirring rod to transfer one drop of solution to a well in the spotting tile that contains iodine
Results
The iodine should turn blue - black if starch is present
Until result
Take a sample every 30 seconds and continue until iodine remains orange
What does it mean when iodine remains orange?
When iodine remains orange - tells us that starch is no longer present and the reaction has been completed
time taken
We record the time for this in our results
Repeat
Repeat the whole experiment several times using different PH Buffers
PH 6, PH 7, PH 8
how is sm. intestine adapted (length)
length of 5 m
large s.a for absorbtion of products of digestion
how is the interior of small intestine adapted
sm. intestine interior has millions of villi
increase s.a for absorbtion of molecules
where are microvilli found and what do they do
microvilli are found on the surface of villi
increase s.a even further
describe another way villi are adapted (blood)
very good blood supply.
blood stream removes products of digestion rapidly
increases concentration gradient
how do villi increase the concentration gradient
they have god blood supply which quickly removes the products of digestion
how are villi adapted ( membrane )
thin membrane,
short diffusion pathway
list all the adaptations of sm.intestine inc villi (5 points)
very long- large s.a for absorbtion
have villi- increase s.a
villi have microvilli
villi have good blood supply
villi have thin membrane
fish have a single circulatory system. describe this
blood pumped from heart to gills
collects o2 in gills(oxygenated)
blood passes straight to organs
oxygen diffuses OUT of blood into body CELLS
blood returns to heart
what is a problem with single circulatory system
blood loses pressure as it passes to gills before organs
blood travels to organs slowly, cant deliver alot of o2
double cirulatory system
heart to lungs
collects o2
o2 blood back to heart
o2 blood pumped to organs
o2 transferred to body cells
deoxygenated blood back to heart
benefit of double circulatory system
blood passes to heart twice,
travels rapidly to body cells
delivers o2 cells need
the heart is an ………. consisting mainly of …….. tissue
the heart is an organ consisting mainly of muscle tissue
name the 4 chambers of the heart
right atrium left atrium
right ventricle left ventricle
the heart and circulatory system
……. are separated from ….. by valves
atria separated from ventricle by valves
what does vena cava do
brings X O2 blood to heart
what does pulmonary artery do
PA takes X O2 blood to lunfs
what does Pulmonary Vein do
brings O2 blood from lungs to heart
what does aorta do
takes O2 blood from heart to body
describe pattern of blood flow through heart
blood enters right and left atrium
atria contract-
blood forced to ventricles
ventricles contract-
blood forced out of heart
what do valves do
valves stop back flow of blood
(in atria when ventricles contract)
The …… ventricle pumps blood to the lungs where
….. …. takes place
The right ventricle pumps blood to the lungs where
gas exchange takes place
The ….. ventricle pumps blood ………
The left ventricle pumps blood around the
rest of the body.
what does the left ventricle do
The left ventricle pumps blood around the
rest of the body.
what does the right ventricle do
The right ventricle pumps blood to the lungs where
gas exchange takes place.
the … side of the heart has a thicker…… …. than the … side
the left side of the heart has a thicker musculer wall then the right side
why does the left side of the heart have a thicker muscular wall then the right side
the left ventricle pumple blood around ENTIRE body.
needs to provide a GREATER force
where do coronary arteries branch out from
coronary arteries branch out from aorta
coronary arteries …… out of ….. and spread out into ….. ……
coronary arteries branch out of aorta and spread out into heart muscle
what is the purpose of the coronary arteries
purpose of coronary arteries is to provide oxygen to muscle cells of heart