Objective 5: List 4 types of fungal stains and organism identified with each

Question 1

Lactophenol cotton blue

Answer 1

• phenol kills the organism, cotton blue stains
• fruiting bodies
• Ex: Histoplasma
• IMAGE

Question 2

Gomori methenamine silver (GMS)

Answer 2

A) yeast cells and hyphae

• Pneumocystis carinii, Aspergillus (a)
• blastomyces dermatitidis (better than with PAS)

B)Silver stain for bacteria and spirochetes

-Warthin-Starry or Steiner: silver impregnated stain for formalin fixed tissue. demonstrate: spirochetes, cat scratch disease (B. henselae)

Question 3

Periodic acid-Schiff (PAS)

Answer 3

• yeast cells and hyphae
• Aspergillus (b) in lung bx illuminates hyphae

Question 4

Mucicarmine (mucin)

Answer 4

-cryptococcus capsule (in lung Bx) -only stains capsule, stains around papillary