Objective 1: Describe how to prepare specimen and perform gram stain

Question 1

Prepare

Answer 1

roll q tip, pipette sample

Question 2

Step 1

Answer 2

+/- cells on the slide

Question 3

Step 2

Answer 3

+/- primary stain crystal violet-stain purple

Question 4

Step 3

Answer 4

+/- stays purple mordant grams iodine (complexes with crystal violet, holds it in the cell)

Question 5

Step 4

Answer 5

decolorizer alcohol (removes lipid layer of cell wall) and/or acetone. Loyola uses 50:50 mix.

POSITIVE: remain purple, not much lipid.

NEGATIVE: become colorless, dissolve/washes away the lipid cell wall

Question 6

Step 5

Answer 6

counterstain SAFRANIN (red stain)

POSITIVE: remain PURPLE

NEGATIVE: stain PINK

Question 7

Observations

Answer 7

observe presence of inflammatory cells