Fluorescent Staining Techniques Flashcards
1
Q
Fluorescence Stain
A
- fluorophores or fluorochromes absorb energy from the nonvisible UV and short visible wavelengths
- they become excited and emit energy in the form of longer visible wavelengts
- color of flo depends on dye and filters on the special microscope
2
Q
Fluorescent Microscopy
A
a. Auramine-rhodamine:Mycobacteria
- flo maintained=acid fast
b. Calcofluor white stain:Fungi
- whitener attaches to chitin in cell membrane walls
c. Fluorescein conjugated antibodies:viruses, bordetella, legionella, pneumocystis
