Objective 4: List organisms that stain positive in acid-fast stains

Question 1

Acid Fast Stains 101: Back to Basics

Answer 1

• designed to stain bacteria with cell walls contain long chain fatty acids
• use heat or detergent to drive stain into cell, once in the cell resists decolorization=AF bacilli

Question 2

**Im Ass so Fast 4(real)!

Answer 2

1. Mycobacteria
2. Nocardia
3. Cryptosporidia
4. Cyclospora

Question 3

Methods (2)

Answer 3

1. Ziehl-Neelsen (heat)

2. Kinyoun (cold stain + detergent)

Question 4

AF Procedure

Answer 4

1. +/- cells on slide
2. +/- primary stain (carbalfuchsin red)-BOTH stain red
3. Decolorizer (HCl, alcohol)
   +: remain red
   -:become colorless
4. Counterstain (methylene blue)
   +: remain RED
   -: stain BLUE

Question 5

What did Mike do?

Answer 5

Mike the Bacterium led the clan to narnia there was a crypt of spores in his way but he cycled around the spores