Nucleus Flashcards
Proteins within the nucleus can be categorized by two types. They are?
Histones and non histones
mRNA, tRNA, rRNA, proteins, chromatin, nucleolus, and the nuclear envelope are all components of…
The nucleus
Small, positively charged basic proteins that form the core of the nucleosome complex
Histones
What are the four characteristics described of Non histones according to lecture?
- Negatively charged acidic proteins
- Regulate transcription
- Involved in DNA repair and replication
- Maintain and convert chromatin structure
When staining heterochromatin, which stain do you use and how does it stain?
for the purpose of the lecture slides. Did not look up to see if there are other stains you can use.
H&E and it stains dark
What are the two types of heterochromatin?
Constitutive and facultative
T/F heterochromatin is not transcriptionally active?
True
T/F Constitutive heterochromatin contains DNA that is not transcribed
True
Euchromatin IS transcriptionally active and stains how with H&E?
Lightly
Ribonucleoproteins, nuclear lamina, and chromatin are associated with?
The inner membrane of the nuclear envelope.
The outer membrane of the nuclear envelope is continuous with the ER, similar in structure to the ER, therefore it must be associated with?…
RIBOSOMES!! You got it dude.
Nuclear lamin codes for at least (#?) proteins.
Lucky 7
How do the three intermediate filaments of the nuclear lamina provide support to the inner membrane of the nuclear envelope?
- during cell division- the lamins become phosphorylated and the nuclear membrane undergoes dissolution
- after cell division- nuclear lamins are dephosphorylated and the membrane is reformed and stabilized
Hutchinson-Gilford Progeria is an example of a mutation involving?
The nuclear lamina
Lamins interact to form?…
Dimers!
