Nucleic Acid Flashcards
Linear polymers built from individual units called nucleotide connected by bonds called phosphodiester linkages
Nucleic acids
is a covalent bond that links nucleotides together
Phosphodiester bond
Are used to pass genetic information from generation to the next
DNA and RNA
Difference between purine & pyrimidine
Purine: two fused ring
Pyrimidine: single ring
Connects sugars of nucleic acids
Phosphodiester linkages
Used to detect phosphate in nucleic acids which involves the addition ammonium molybdate with dilute nitric acid resulting in bright yellow precipitate of ammonium phosphomolybdate
Test for phosphate
The Bial’s reagent contains concentrated hydrochloric acid which dehydrates pentose into furfural and further reacts with orcinol and ferric chloride yielding a blue-green product
Test for ribose
Acidic conditions convert deoxyribose to a molecule that binds with diphenylamine to form a blue complex
Test for deoxyribose
Chemical test to identify the presence of caffeine and purine derivatives in a sample
Murexide test
Reaction of KOH solution produces a yellowish-brown precipitate after heating which indicates a positive result
Murexide test
Produces a green coloration when sample is treated with bromine water
The addition of barium hydroxide will turn the solution purple
Test for pyrimidines
Stores genetic information
DNA
Transcribe the information stored in DNA process
RNA
