Normal Oral Microflora Flashcards

1
Q

Oral cavity microbial habitats

A

teeth
gingival sulci
attached gingiva
lips, tongue, cheeks
hard and soft palate

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2
Q

Normal flora

A

called commensals

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3
Q

Supragingival bacteria

A

predominantly streptococci
mutans
salivarius
mitis
anginosus

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4
Q

Subgingival bacteria

A

actinomyces
prevotella
prophyromonas
fusobacterium
veillonella

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5
Q

Gram (+) bacteria

A

Cocci:
Streptococcus –> Mutans, salivarius, mitis, anginosus

Bacilli and filaments:
Lactobacilli, eubacteria, propionibacterium acnes and propionicus, actinomyces

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6
Q

What are the four groups of streptococcus

A

mutans, salivarius, mitis, anginosus

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7
Q

What is the the mutans group

A

colonizes tooth or denture surfaces
rapidly produces acid
can grow in an acidic environment

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8
Q

What is the primary pathogen in dental caries

A

Streptococcus mutans

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9
Q

What can streptococcus mutans cause

A

root surface caries in elderly
pit and fissure caries
smooth surface (enamel) caries

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10
Q

What is the important virulence property of mutans group

A

ability to form bioflim

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11
Q

What is salivarious group

A

found on mucosal surfaces, including the tongue

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12
Q

What are the two groups of salivarius group

A

strept. salivarious & strept. vestibularis

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13
Q

Strept. Salivarious

A

Opportunistic pathogen capable of infecting immunocomprised patients

Strongly cariogenic, but less than S. mutans

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14
Q

Strept. vestibularis

A

Normal inhabitant of oral vestibules

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15
Q

Mitis group

A

S. sanguinis, strept. gordonii, strept. mitis, and strept. oralis may become opportunistic pathogens and cause infective endocarditis

early colonizers in biofilm formatoin

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16
Q

What mitis group bacteria is not an opportunistic pathogen

A

S. mitis

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17
Q

What is the anginosus group

A

Found in dental plaque and mucosal surfaces
opportunistic pathogens
involved in maxillofacial infections
includes: strept. anginosus, strept. intermedius, and strept. constellatus

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18
Q

What are the gram (+) cocci bacteria

A

Streptococcus: Mutans, Salivarius, Mitis, Anginosus

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19
Q

What are gram (+) bacilli and filaments

A

Lactobacilli, Eubacteria, Propionbacterium, Actinomyces

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20
Q

Lactobacilli

A

Constitute about 1% of the total cultivable microflora in the oral cavity

Increases in advanced caries lesions of the enamel and root surface

Acidogenic and also acid tolerant

Testing for lactobacilli in the saliva of patients is one way to monitor patients’ dietary intake of carbohydrates

21
Q

Eubacteria

A

pleomorphic, obligate anaerobes found in dental plaque

constitute more than 50% of the anaerobes of periodontal pockets

22
Q

propionibacterium

A

propionibacterium acnes and propionibacterium propionicus

strict anaerobic bacilli found in dental plaque

23
Q

Actinomyces species

A

Major compoenet in dental plaque

In subgingival microbiota of healthy individuals

Actinomyces israelii can be an opportunistic pathogen: can cause actinomycosis chronic inflammation in the orofacial region

24
Q

Gram (-) bacteria

A

cocci: veillonella species
bacilli: aggregatibacteria actino,
fusobacterium
porphyromonas prevotella

Spiral shaped: campylobacter rectus,
treopnema

Pleomorphic: Tannerella forsythia

25
Veillonella species
Gram (-) cocci Anaerobic gram (-) cocci Found mostly in plaque
26
AAA
gram (-) bacilli found occasionally in subgingival plaque associated with aggressive periodontitis stimulate bone resorption
27
Fusobacterium species
gram (-) bacilli Strict anaerobes Fusobacterium nucleatum: present in low numbers in healthy subgingival dental biofilm enriched in periodontal pockets key bridging organism between the early colonizers and periodontal pathogens opportunistic pathogen due to its association with systemic diseases
28
Porphyromonas
gram (-) bacilli Utilize proteins and peptides for their metabolism Porphyromas gingivalis: found almost exclusively at subgingival sites, especially in advanced periodontal lesions; major flora in dental plaque Porphyromas endodontalis: identified primarily in infected root canals
29
Prevotella
gram (-) bacilli Strict anaerobes P. intermedia associated with periodontitis and endodontic infections Major flora in dental plaque
30
Gram (-) spiral-shaped bacteria
Campylobacter rectus Treponema
31
Campylobacter rectus
strict anaerobe and is often found in sites of periodontal disease agent of chronic periodontal disease
32
Treponema
Strict anaerobes spirochete T.denticola: associated with progression of periodontal diseases increase in number in advanced periodontal disease
33
Gram (-) pleomorphic bacteria
Tannerella forsythia
34
Tannerlla forsythia
gram (-) anaerobe inhabits subgingival cavity initiates connective tissue destruction and alveolar bone resorption in periodontal disease
35
Fungi: Candida species
Constitute the largest fraction of fungi in the mouth prevalence of candida in asymptomatic adults is 2-71% and 100% in immunocompromised individuals and those on broad-spectrum antibiotics Most common site is dorsum of the tongue Use of dentures and orthodontic appliances increases the incidence Candida albicans: most common species
36
Viruses
Herpes simplex virus type 1 Coxsackievirus Human papillomavirus types 2,4,6,11 and 16 Hepatitis B and HIV
37
Herpes simplex virus type I
Most frequently found in saliva and orofacial region cause of cold sores, which occur when the latent virus in the trigeminal nerve is activated by stress or ultraviolet light
38
Coxsackievirus
detected in saliva and oral epithelium
39
True/false:Human papillomavirus types 2,4,6,11, and 16 are detected in oral lesions of patients with HIV/AIDS
True
40
True/false: Hepatitis B and HIV in saliva may pose a cross-infection threat, emphasizing the need for universal precautions
True
41
Protozoa
Trichomonas tenax and entamoeba gingivalis found in frequently in oral cavity only 2% of healthy population carries T. tenax but increases to 21% in patients with periodontal disease
42
Factors contributing to disease
salivary flow rate: can be affected by medications diet tobacco use integrity of the immune system
43
Main oral habitats:
Mucosal surface teeth saliva gingival crevicular fluid
44
Dorsum of tongue
Has papillary structures that provide a niche for bacteria that would otherwise be removed by salivary flow Environment suitable for gram (-), obligately anaerobic bacteria that are involved in periodontal disease and malodor (halitosis)
45
Teeth
Constitute a non-shedding surface for bacterial colonization Bacteria and their polysaccharide products accumulate on tooth surface to produce dental plaque, which is generated both in health and in disease More present in pits and fissures Subgingival surfaces are in a more anaerobic environment that supragingival surfaces
46
Saliva
Can clear potentially damaging acids produced by plaque bacteria after metabolizing carbohydrates saliva buffers the pH between 6.75 and 7.25
47
Proteins and glycoproteins in saliva
generate the acquired pellicle on tooth surfaces provide nutrients for normal oral microflora Aggregate microorganisms to facilitate their removal by swallowing Inhibit the growth of exogenous microoganisms
48
Gingival crevicular fluid
Can remove non-adherent bacteria, introduce igG and neutrophils, and provide peptides and carbohydrates that bacteria can use for their metabolism The pH increases in the presence of gingivitis and periodontitis, providing a favorable environment for periodontopathogens such as p. gingivalis and P. intermedia
49
Appliances
Prosthodontic and orthodontic appliances may act as reservoirs for bacteria and yeasts one of the causes of candida-associated denture stomatitis is poor denture hygiene