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BACTE (CHAPTER 16) > Nonsporeforming, Nonbranching, Catalse (-) > Flashcards

Nonsporeforming, Nonbranching, Catalse (-) Flashcards

1
Q

Only sp. of Erysipelothrix that cause disease to human.

A

Erysipelothrix rhusiopathiae

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2
Q

3 species that are non-spore-forming, Nonbranching Catalase (-).

A

Erysipelothrix rhusiopathiae
Arcanobacterium
Gardnella vaginalis

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3
Q

Gram+
Catalae-
Pleomorphic
Tend to form long filaments

A

Erysipelothrix rhusiopathiae

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4
Q

Erysipelothrix rhusiopathiae can be acquired through

A

Occupational exposure (handling fish and animal products)

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5
Q

T or F
The route of infection of E. rhusiopathiae is through mouth.

A

F. - cuts or scratches

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6
Q

E. rhusiopathiae is resistant to ____.

A

Salting, picking, smoking

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7
Q

2 common clin. Infection of E. rhusiopathiae.

A

Erysipeloid
Septicemia

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8
Q

Which is not true about erysipeloid?
A. Usually resemble to strep. Erysipelas
B. It contains pus
C. Painful and swollen
D. Sharp and slightly defined
F. Purplish-red zone

A

B.

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9
Q

Disease casue by E. rhusiopathiae that is usually associated with endocarditis.

A

Septicemia

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10
Q

Most common infection caused by E. rhusiopathiae.

A

Erysipeloid

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11
Q

Identify what bacteria
- thin,rod shaped, gram + that can form long filaments. It is arragein single, short chains, or in V shape.

A

E. rhusiopathiae

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12
Q

T or F
E. rhusiopathiae is difficult to decolorized.

A

F. - easily decolorize.

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13
Q

Specimen used in isolation of E. rhusiopathiae.

A

Tissue
Aspiration in skin

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14
Q

Find the mistakes
Inoculate to NA w/ 2% glucose
Incubate in 5% CO2 at 37°C

A

1% glucose
35°C

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15
Q

Appearance of colonies of E. rhusiopathiae in SBA.

A

Non-hemolytic
Pinpoint

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16
Q

After 48hrs of incubation, 2 distinct colonies are form. What are they?

A

Smaller (smooth, younger)
Larger (rough, older)

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17
Q

The hemolysis of E. rhusiopathiae in SBA is ___.

A

Alpha hemolysis

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18
Q

T or F
E. rhusiopathiae is nonmotile, pleomorphic, catalase (-)

A

True

19
Q

In E. rhusiopathiae, what is the reaction in different biochem tests?
H25
VP
Urease
Esculin hydrolysis

A

Positive
H25
Negative
VG
Urease
Esculin hydrolysis

20
Q

Appearance of E. rhusiopathiae in gelatine stab culture.

A

Test tube brush pattern.

21
Q

What are the medical inportant Arcanobacterium ?

A

A. haemolyticum
A. pyogenes
A. bernardiae

22
Q

An Arcanobacterium sp. that is animalnpathogen and best known for causing infection in cattle

A

A. pyogenes

23
Q

A. haemolyticum is formely known as __.

A

Corynebacterium haemolyticum

24
Q

Recovered from 10-20 yrs. Old with pharyngitis

A

A. haemolyticum

25
Q

Develop pruritic, scarlatiniform rash, desquamation of the skin of hands and feet.

A

Arcanobacterium haemolyticum

26
Q

What is observed when the colony of A. haemolyticum is remove?

A

Black opaque dot
And pitting of agar

27
Q

A. haemolyticum is both lipase and lecithinase ___.

A

Positive.

28
Q

Result of A. haemolyticum in CAMP test.

A

Reverse CAMP reaction the arrow head has no hemolysis

29
Q

Concept of CAMP Test in A. haemolyticum.

A

It inhibits reverse CAMP rxn. Because the hemolysis produced by a Beta-lysin-producing S. aureus is inhibited by a phoppholipase D excreted by A. haemolyticum

30
Q

Drug of choice for A. haemolyticum.

A

Erythromycin

31
Q

Short, pleomorphic, gram+ and rod/coccobacillus that often gram-variable or gram(-)

A

Gardnerella vaginalis

32
Q

T or F
Gardnerella vaginalis is the only sp. In this genus.

A

True

33
Q

Microflora in Female genital tract.

A

G. vaginalis

34
Q

This infection is only cause by G. vaginalis.

A

Bacterial vaginalis

35
Q

Describe as malddorous discharge and vaginal ph 7 than 4.5

A

Bacterial vaginalis

36
Q

Effect of Bacterial vaginalis

A

Reduction of Lactobacillus
Increase of pH
Increase G. vaginalis

37
Q

Drug used for Bacterial vaginalis

A

Metronidazole

38
Q

Large squamous epithelial cells with gram + and gram variable bacilli and coccobacilli clustered in edge in bacterial vaginalis infection is called??

A

Clue cells

39
Q

___ for gram stain vaginal smear is more accurate compared to culture in diagnosing BV.

A

Nugent scoring system

40
Q

G. vaginalis grows best at what concentration of CO2 and at what temp?

A

5-7% CO2 and 35-37°C

41
Q

Medium of choice for G. vaginalis .

A

Human blood Bilayer Tween (HBT) agar.

42
Q

Appearance of G. vaginalis in HBT agar.

A

B hemolytic, small, gray, opaque

43
Q

Biochemical reaction of G. vaginalis
In
Catalase
Oxidase
Hippurate hydrolysis

A

Catalase (-)
Oxidase (-)
Hippurate hydrolysis (+)

BACTE (CHAPTER 16) (4 decks)

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