Nonsporeforming, Nonbranching, Catalse (-) Flashcards
Only sp. of Erysipelothrix that cause disease to human.
Erysipelothrix rhusiopathiae
3 species that are non-spore-forming, Nonbranching Catalase (-).
Erysipelothrix rhusiopathiae
Arcanobacterium
Gardnella vaginalis
Gram+
Catalae-
Pleomorphic
Tend to form long filaments
Erysipelothrix rhusiopathiae
Erysipelothrix rhusiopathiae can be acquired through
Occupational exposure (handling fish and animal products)
T or F
The route of infection of E. rhusiopathiae is through mouth.
F. - cuts or scratches
E. rhusiopathiae is resistant to ____.
Salting, picking, smoking
2 common clin. Infection of E. rhusiopathiae.
Erysipeloid
Septicemia
Which is not true about erysipeloid?
A. Usually resemble to strep. Erysipelas
B. It contains pus
C. Painful and swollen
D. Sharp and slightly defined
F. Purplish-red zone
B.
Disease casue by E. rhusiopathiae that is usually associated with endocarditis.
Septicemia
Most common infection caused by E. rhusiopathiae.
Erysipeloid
Identify what bacteria
- thin,rod shaped, gram + that can form long filaments. It is arragein single, short chains, or in V shape.
E. rhusiopathiae
T or F
E. rhusiopathiae is difficult to decolorized.
F. - easily decolorize.
Specimen used in isolation of E. rhusiopathiae.
Tissue
Aspiration in skin
Find the mistakes
Inoculate to NA w/ 2% glucose
Incubate in 5% CO2 at 37°C
1% glucose
35°C
Appearance of colonies of E. rhusiopathiae in SBA.
Non-hemolytic
Pinpoint
After 48hrs of incubation, 2 distinct colonies are form. What are they?
Smaller (smooth, younger)
Larger (rough, older)
The hemolysis of E. rhusiopathiae in SBA is ___.
Alpha hemolysis
T or F
E. rhusiopathiae is nonmotile, pleomorphic, catalase (-)
True
In E. rhusiopathiae, what is the reaction in different biochem tests?
H25
VP
Urease
Esculin hydrolysis
Positive
H25
Negative
VG
Urease
Esculin hydrolysis
Appearance of E. rhusiopathiae in gelatine stab culture.
Test tube brush pattern.
What are the medical inportant Arcanobacterium ?
A. haemolyticum
A. pyogenes
A. bernardiae
An Arcanobacterium sp. that is animalnpathogen and best known for causing infection in cattle
A. pyogenes
A. haemolyticum is formely known as __.
Corynebacterium haemolyticum
Recovered from 10-20 yrs. Old with pharyngitis
A. haemolyticum
Develop pruritic, scarlatiniform rash, desquamation of the skin of hands and feet.
Arcanobacterium haemolyticum
What is observed when the colony of A. haemolyticum is remove?
Black opaque dot
And pitting of agar
A. haemolyticum is both lipase and lecithinase ___.
Positive.
Result of A. haemolyticum in CAMP test.
Reverse CAMP reaction the arrow head has no hemolysis
Concept of CAMP Test in A. haemolyticum.
It inhibits reverse CAMP rxn. Because the hemolysis produced by a Beta-lysin-producing S. aureus is inhibited by a phoppholipase D excreted by A. haemolyticum
Drug of choice for A. haemolyticum.
Erythromycin
Short, pleomorphic, gram+ and rod/coccobacillus that often gram-variable or gram(-)
Gardnerella vaginalis
T or F
Gardnerella vaginalis is the only sp. In this genus.
True
Microflora in Female genital tract.
G. vaginalis
This infection is only cause by G. vaginalis.
Bacterial vaginalis
Describe as malddorous discharge and vaginal ph 7 than 4.5
Bacterial vaginalis
Effect of Bacterial vaginalis
Reduction of Lactobacillus
Increase of pH
Increase G. vaginalis
Drug used for Bacterial vaginalis
Metronidazole
Large squamous epithelial cells with gram + and gram variable bacilli and coccobacilli clustered in edge in bacterial vaginalis infection is called??
Clue cells
___ for gram stain vaginal smear is more accurate compared to culture in diagnosing BV.
Nugent scoring system
G. vaginalis grows best at what concentration of CO2 and at what temp?
5-7% CO2 and 35-37°C
Medium of choice for G. vaginalis .
Human blood Bilayer Tween (HBT) agar.
Appearance of G. vaginalis in HBT agar.
B hemolytic, small, gray, opaque
Biochemical reaction of G. vaginalis
In
Catalase
Oxidase
Hippurate hydrolysis
Catalase (-)
Oxidase (-)
Hippurate hydrolysis (+)
