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BACTE (CHAPTER 16) > Non-sporing,Nonbranching,catalase (+) > Flashcards

Non-sporing,Nonbranching,catalase (+) Flashcards

1
Q

Corynebacterium can be divided into 2 species. What are they?

A

Nonlipophilic and lipophilic

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2
Q

Corynebacteria sp. That are considered fastidious and grow slowly on standard culture.

A

Lipophylic corynebcateria

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3
Q

Additional of ____ in culture medium enhance the grow of Lipophylic Corynebacteria.

A

Lipid

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4
Q

Which is/are true in Gram stain of Corynebacterium?
A. Gram postive
B. Catalase positve
C. Slightly curved
D. Club-shape or coryneform

A

All answers are correct.

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5
Q

They are non-spore-forming and catalase (+) bacilli.

A

Listeria and Corynebacterium

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6
Q

Non-spore-forming and Catalase (-)

A

Erysipelothrix and Lactobacillus

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7
Q

Spore forming and gram postive bacilli.

A

Bacilli spp. (Aerobe)
Clostridium spp. (Anaerobe)

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8
Q

Non-spore-forming, nonbranching catalase (+) bacilli.

A

Corynebacterium
Rothia
Listeria monocytogenes

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9
Q

Disease cause by C. diphtheriae.

A

Diphtheria

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10
Q

Most significant pathogen of Corynebacterium.

A

C. diptheriae

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11
Q

Nondiphtheria C. Spp. is dismissed as ____ .

A

commensals

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12
Q

Major virulence factor of C. diptheriae.

A

Diptheria toxins

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13
Q

Diptheria toxin cam from what?

A

Lysogenic B-phage

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14
Q

Lysogenic B-phage carries ___.

A

Tox gene

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15
Q

T or F
Only toxin-producing C. diptheriae cause diphtheria.

A

True

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16
Q

Corynebacterium spp. That can produce toxins.

A

C. diptheriae
C. pseudotuberculosis
C. ulcerans

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17
Q

The two fragments of Diptheria toxins.

A

Fragment A and B

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18
Q

Why can diphtheria toxin be toxic in humans?

A

This toxins block the protein synthesis in eukaryotic cells.

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19
Q

T or F
Diphtheria toxin secreted by bacterial cell in nontoxic until they react with trypsin.

A

True

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20
Q

Fragment A is responsible for?

A

Cytotoxicity

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21
Q

Fragment B is for?

A

Allows the Fragment A to enter in cytoplasm

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22
Q

Two different form of disease cause by C. diptheriae.

A

Respiratory diphtheria
Cutaneous diphtheria

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23
Q

Respiratory diphtheria can be transmitted through___.

A

Spread of droplets and hand-to-mouth contact

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24
Q

T or F
Corynebacterium is gram + and motile bacteria.

A

False-nonmotile

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25
Q

Vaccine for respiratory diphtheria.

A

DPT vaccine (diphtheriapertasis and tetanus vaccine)

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26
Q

Most common site of respiratory diphtheria.

A

Tonsil and pharynx

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27
Q

Cell necrosis +exudate=

A

Tough gray to white pseudomembrane

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28
Q

Respiratory diphtheria can cause problem except:
A. Suffocation
B. Systemic disease
C. Non-healing ulcer
D. Demyelinating peripheral neutritis

A

C.

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29
Q

This consists of non-healing ulcers and a dirty gray membrane.

A

Cutaneous diphtheria

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30
Q

Treatment for diptheria infections

A

Anti-toxin
Penicillin
Erythromycin

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31
Q

Which is not part of the microscopic identity of Corynebacterium?
A. Pleimorphic
B. Palisades
C. Club-shape swelling
D. Beaded form
E. V or L formation
F. In clusters

A

F.

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32
Q

In microscopic examination of Corynebacterium, the metachromic area is called?

A

Babes-Ernst granules

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33
Q

The babes-ernst granules are accumulation of ___.

A

Polymerized polyphosphates

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34
Q

T or F
C. diptheriae is facultative aerobes.

A

False- facultative anaerobes

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35
Q

The growth of C. diptheriae in culture media requires ____ .

A

8 essential amino acids

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36
Q

Among the 8 AA, what is the most required one by C. diphtheriae for growth?

A

Cysteine

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37
Q

Which media is not good for the growth of C. diptheriae?
A. Loeffler medium/ Pai agar
B. CTBA
C. SBA
D. MAC

A

D.

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38
Q

Modified medium of Tinsdale medium.

A

Cystine-tellurite blood agar (CTBA)

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39
Q

What color of colonies if C. diphtheriae grows in CTBA ?

A

Black-brown

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40
Q

CTBA contains what?

A

Sheep RBCs
Bovine serum
Cystine
Potassium tellurite

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41
Q

Content of CTBA that inhibits the growth of other non-coryneform bacteria.

A

Potassium tellurite

42
Q

What does the brown halo that surround the colony in CTBA media?

A

It is due to cystinase activity

43
Q

Only Corynebacterium that produce brown halo in CTBA.

A

C. diphtheriae
C. ulcerans
C. pseudotuberculosis

44
Q

Vitro diphtheria toxin detection proceudre is an immunodiffusion test.

A

Elek test

45
Q

In elek test , filter paper strip impregnated with ___.

A

Diphtheria toxin

46
Q

if both concentration of Ag and Ab is equal.(Zone of equivalent )

A
  • they will form precipitin line /band
47
Q

Merge precipitin line

A

identical toxin product

48
Q

If cross out precipitiline line

A

both produce toxin but different strain and bacteria

49
Q

Spur formation

A

-partial identity

50
Q

Most frequent Corynebacterium sp. from human clinical material.

A

-Corynebacterium amycolatum

51
Q

C. jeikeium name after

A

Johnson and Kaye

52
Q

Most commonn Corynebacterium associated with prosthetic valve endocarditis .

A

C. jeikeium

53
Q

Stric anaerobe
Non-hemotytic
Urease positve
Reduces nitrate
Drug of choice: vancomycin

A

C. jeikeium

54
Q

C. spp. that is normal flora in nasopharynx.

A

Corynebacterium pseudodiphtheriticum

55
Q

T or F
C. pseudodiphtheriticum a pleomorphic morphology.

A

True

56
Q

They appear palisades, they grow in standard laboratory media, reduce nitrate and produces urease

A

C. psedodipththeriticum

57
Q

They appear palisades, they grow in standard laboratory media, reduce nitrate and produces urease

A

C. psedodipththeriticum

58
Q

Type of C. sp. That is primarily a veterenary pathogen and associated contact with sheep.

A
  • C. pseudotuberculosis
59
Q

C. pseudotuberculosis causes ____.

A

granulomatous lymphadenitis

60
Q

It produces demonecrotic toxin and its appearance in SBA is small yellow-white colonies.

A
  • C. pseudotuberculosis
61
Q

Nonlipophilic, pleomorphic, small, shiny, convex colonies. Resistant to penicillin.

A

-C. striatum

62
Q

A veterinary pathogen causing mastitis in cattle and other domestic animals

A

C. ulcerans

63
Q

It can be acquired through ingestion of unpasteurized dairy product.

A

C. ulcerans

64
Q

Does not reduce nitrate differentiating from C. diphtheriae, and it is urease positive

A

C. ulcerans

65
Q

Common C. spp. associated with UTI.

A

C. urealyticum

66
Q

C. Bacteria urine isolates with pinpoint, nonhemolytic white colonies and coryneform morphology.

A

C. urealyticum

67
Q

C. spp that is nitrate negative, catalase positve and rapidly urease positive.

A

C. ureayticum

68
Q

C. sp. That is dry, grows well in SBA and yellow to tan colonies.

A

C. xerosis

69
Q

Gram positive cocci that can appear rod like belong to Micrococcaceae

A

Rothia

70
Q

They are normal human oropharyngeal microbes

A

Rothia

71
Q

Two spp. Of Rothia significant to human

A

Rothia demtocariosa
Rothia mucilaginosa

72
Q

Rothia that is found in saliva and supragingival plague

A

Rothia dentocariosa

73
Q

Rothia that resemble as corynefomr basilli and facultative actinomycetes.

A

Rothia dentocariosa

74
Q

Only listeria spp that is significant to human.

A

Listeria monocytogenes

75
Q

Listeria that is an animal pathogen

A

L. ivanovii

76
Q

In which L. monocytogenes can infect?
A. Immunocompromised patient
B. Neonates
C. Pregnant women
D. Healthy individual

A

All of the above

77
Q

Virulence factors of L. monocytogenes

A

Listeriosin O
Catalase
Superoxide dismutase
Phospholipase C
Surface protein

78
Q

Virulence factor of L. monocytogenes that damage phagosome membrane

A

Listeriolysin O

79
Q

Virulence factors od L. monocytogenes that induces phagocytosis through increased adhesion and penetration of the cells

A

Surface protein (p60)

80
Q

Clinical infection in which via ingestion of contaminated food w subsequent spread through intestine .

A

Listeriosis

81
Q

Which among is not true about manifeststion of L. monocytogenes?
A. Tropism for CNS
B. Pregnant women
C. Common in newborn
D. Immunocompromised
E. Healthy person

A

E.

82
Q

The listeriosis in pregnant women is common seen during ____.

A

Third trimesters

83
Q

What is the effect of Listeriosis in pregnant women?

A

Can lead to spontaneous abortion and stillborn neonates

84
Q

Two forms of neonatal listeriosis?

A

Early onset infection
Late onset infection

85
Q

Manifestation in early onset infection of listeriosis in neonates.

A

Sepsis

86
Q

Manifestation of late onset infection of listeriosis in neonates.

A

Meningitis

87
Q

Most common Manifestation in immunosuppresed host.

A

CNS infection and Endocarditis

88
Q

T or F
L. monocytogenes appear as gram-positive and gram variable in older culture

A
89
Q

L. monocytogenes resembles ____ in coccoid form and _____ in bacillus form

A

Streptococcus
Corynebacterium

90
Q

What mediums does the L. monocytogenes grows well?

A

CHOC
SBA
NA
Brain-Heart infusion
Thioglycolate

91
Q

T or F
L. monocytogenes is slightly microphilic.

A

F- slightly capnophilic

92
Q

Characteristics of L. monocytogenes colonies.

A

Small
Round
Translucent
Smooth

93
Q

The colonies and hemolysis of L. monocytogenes resembles ____.

A

S. agalactiae

94
Q

Optimal growth temp. Of L. monocytogenes.

A

30-35°C

95
Q

Wide range growth of L. monocytogenes.

A

0.5-45°C

96
Q

What is the temp. Of cold enrichment techniques in L. monocytogenes?

A

4°C

97
Q

In wet mount preparation, the L. monocytogenes excibit

A

tumbling motility

98
Q

In motility medium, the characteristics of L. monocytogenes is ___.

A

Umbrella pattern

99
Q

What is the rxn. Of L. monocytogenes in hippurate hydrolysis, bile esculin hydrolysis , and in CAMP factor?

A

Positive

100
Q

In CAMP reaction, what is used to produce block type hemolysis in L. monocytogenes?

A

Rhodococcus equi

101
Q

In CAMP test, what spp. Of bacteria want to differentiate from L. monocytogenes?

A

Listeria spp.

102
Q

L. monocytogenes is differentiated from S. agalactiae and enterococci by ___.

A

Positive catalase test and motility

BACTE (CHAPTER 16) (4 decks)

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