Non-sporing,Nonbranching,catalase (+) Flashcards
Corynebacterium can be divided into 2 species. What are they?
Nonlipophilic and lipophilic
Corynebacteria sp. That are considered fastidious and grow slowly on standard culture.
Lipophylic corynebcateria
Additional of ____ in culture medium enhance the grow of Lipophylic Corynebacteria.
Lipid
Which is/are true in Gram stain of Corynebacterium?
A. Gram postive
B. Catalase positve
C. Slightly curved
D. Club-shape or coryneform
All answers are correct.
They are non-spore-forming and catalase (+) bacilli.
Listeria and Corynebacterium
Non-spore-forming and Catalase (-)
Erysipelothrix and Lactobacillus
Spore forming and gram postive bacilli.
Bacilli spp. (Aerobe)
Clostridium spp. (Anaerobe)
Non-spore-forming, nonbranching catalase (+) bacilli.
Corynebacterium
Rothia
Listeria monocytogenes
Disease cause by C. diphtheriae.
Diphtheria
Most significant pathogen of Corynebacterium.
C. diptheriae
Nondiphtheria C. Spp. is dismissed as ____ .
commensals
Major virulence factor of C. diptheriae.
Diptheria toxins
Diptheria toxin cam from what?
Lysogenic B-phage
Lysogenic B-phage carries ___.
Tox gene
T or F
Only toxin-producing C. diptheriae cause diphtheria.
True
Corynebacterium spp. That can produce toxins.
C. diptheriae
C. pseudotuberculosis
C. ulcerans
The two fragments of Diptheria toxins.
Fragment A and B
Why can diphtheria toxin be toxic in humans?
This toxins block the protein synthesis in eukaryotic cells.
T or F
Diphtheria toxin secreted by bacterial cell in nontoxic until they react with trypsin.
True
Fragment A is responsible for?
Cytotoxicity
Fragment B is for?
Allows the Fragment A to enter in cytoplasm
Two different form of disease cause by C. diptheriae.
Respiratory diphtheria
Cutaneous diphtheria
Respiratory diphtheria can be transmitted through___.
Spread of droplets and hand-to-mouth contact
T or F
Corynebacterium is gram + and motile bacteria.
False-nonmotile
Vaccine for respiratory diphtheria.
DPT vaccine (diphtheriapertasis and tetanus vaccine)
Most common site of respiratory diphtheria.
Tonsil and pharynx
Cell necrosis +exudate=
Tough gray to white pseudomembrane
Respiratory diphtheria can cause problem except:
A. Suffocation
B. Systemic disease
C. Non-healing ulcer
D. Demyelinating peripheral neutritis
C.
This consists of non-healing ulcers and a dirty gray membrane.
Cutaneous diphtheria
Treatment for diptheria infections
Anti-toxin
Penicillin
Erythromycin
Which is not part of the microscopic identity of Corynebacterium?
A. Pleimorphic
B. Palisades
C. Club-shape swelling
D. Beaded form
E. V or L formation
F. In clusters
F.
In microscopic examination of Corynebacterium, the metachromic area is called?
Babes-Ernst granules
The babes-ernst granules are accumulation of ___.
Polymerized polyphosphates
T or F
C. diptheriae is facultative aerobes.
False- facultative anaerobes
The growth of C. diptheriae in culture media requires ____ .
8 essential amino acids
Among the 8 AA, what is the most required one by C. diphtheriae for growth?
Cysteine
Which media is not good for the growth of C. diptheriae?
A. Loeffler medium/ Pai agar
B. CTBA
C. SBA
D. MAC
D.
Modified medium of Tinsdale medium.
Cystine-tellurite blood agar (CTBA)
What color of colonies if C. diphtheriae grows in CTBA ?
Black-brown
CTBA contains what?
Sheep RBCs
Bovine serum
Cystine
Potassium tellurite
Content of CTBA that inhibits the growth of other non-coryneform bacteria.
Potassium tellurite
What does the brown halo that surround the colony in CTBA media?
It is due to cystinase activity
Only Corynebacterium that produce brown halo in CTBA.
C. diphtheriae
C. ulcerans
C. pseudotuberculosis
Vitro diphtheria toxin detection proceudre is an immunodiffusion test.
Elek test
In elek test , filter paper strip impregnated with ___.
Diphtheria toxin
if both concentration of Ag and Ab is equal.(Zone of equivalent )
- they will form precipitin line /band
Merge precipitin line
identical toxin product
If cross out precipitiline line
both produce toxin but different strain and bacteria
Spur formation
-partial identity
Most frequent Corynebacterium sp. from human clinical material.
-Corynebacterium amycolatum
C. jeikeium name after
Johnson and Kaye
Most commonn Corynebacterium associated with prosthetic valve endocarditis .
C. jeikeium
Stric anaerobe
Non-hemotytic
Urease positve
Reduces nitrate
Drug of choice: vancomycin
C. jeikeium
C. spp. that is normal flora in nasopharynx.
Corynebacterium pseudodiphtheriticum
T or F
C. pseudodiphtheriticum a pleomorphic morphology.
True
They appear palisades, they grow in standard laboratory media, reduce nitrate and produces urease
C. psedodipththeriticum
They appear palisades, they grow in standard laboratory media, reduce nitrate and produces urease
C. psedodipththeriticum
Type of C. sp. That is primarily a veterenary pathogen and associated contact with sheep.
- C. pseudotuberculosis
C. pseudotuberculosis causes ____.
granulomatous lymphadenitis
It produces demonecrotic toxin and its appearance in SBA is small yellow-white colonies.
- C. pseudotuberculosis
Nonlipophilic, pleomorphic, small, shiny, convex colonies. Resistant to penicillin.
-C. striatum
A veterinary pathogen causing mastitis in cattle and other domestic animals
C. ulcerans
It can be acquired through ingestion of unpasteurized dairy product.
C. ulcerans
Does not reduce nitrate differentiating from C. diphtheriae, and it is urease positive
C. ulcerans
Common C. spp. associated with UTI.
C. urealyticum
C. Bacteria urine isolates with pinpoint, nonhemolytic white colonies and coryneform morphology.
C. urealyticum
C. spp that is nitrate negative, catalase positve and rapidly urease positive.
C. ureayticum
C. sp. That is dry, grows well in SBA and yellow to tan colonies.
C. xerosis
Gram positive cocci that can appear rod like belong to Micrococcaceae
Rothia
They are normal human oropharyngeal microbes
Rothia
Two spp. Of Rothia significant to human
Rothia demtocariosa
Rothia mucilaginosa
Rothia that is found in saliva and supragingival plague
Rothia dentocariosa
Rothia that resemble as corynefomr basilli and facultative actinomycetes.
Rothia dentocariosa
Only listeria spp that is significant to human.
Listeria monocytogenes
Listeria that is an animal pathogen
L. ivanovii
In which L. monocytogenes can infect?
A. Immunocompromised patient
B. Neonates
C. Pregnant women
D. Healthy individual
All of the above
Virulence factors of L. monocytogenes
Listeriosin O
Catalase
Superoxide dismutase
Phospholipase C
Surface protein
Virulence factor of L. monocytogenes that damage phagosome membrane
Listeriolysin O
Virulence factors od L. monocytogenes that induces phagocytosis through increased adhesion and penetration of the cells
Surface protein (p60)
Clinical infection in which via ingestion of contaminated food w subsequent spread through intestine .
Listeriosis
Which among is not true about manifeststion of L. monocytogenes?
A. Tropism for CNS
B. Pregnant women
C. Common in newborn
D. Immunocompromised
E. Healthy person
E.
The listeriosis in pregnant women is common seen during ____.
Third trimesters
What is the effect of Listeriosis in pregnant women?
Can lead to spontaneous abortion and stillborn neonates
Two forms of neonatal listeriosis?
Early onset infection
Late onset infection
Manifestation in early onset infection of listeriosis in neonates.
Sepsis
Manifestation of late onset infection of listeriosis in neonates.
Meningitis
Most common Manifestation in immunosuppresed host.
CNS infection and Endocarditis
T or F
L. monocytogenes appear as gram-positive and gram variable in older culture
L. monocytogenes resembles ____ in coccoid form and _____ in bacillus form
Streptococcus
Corynebacterium
What mediums does the L. monocytogenes grows well?
CHOC
SBA
NA
Brain-Heart infusion
Thioglycolate
T or F
L. monocytogenes is slightly microphilic.
F- slightly capnophilic
Characteristics of L. monocytogenes colonies.
Small
Round
Translucent
Smooth
The colonies and hemolysis of L. monocytogenes resembles ____.
S. agalactiae
Optimal growth temp. Of L. monocytogenes.
30-35°C
Wide range growth of L. monocytogenes.
0.5-45°C
What is the temp. Of cold enrichment techniques in L. monocytogenes?
4°C
In wet mount preparation, the L. monocytogenes excibit
tumbling motility
In motility medium, the characteristics of L. monocytogenes is ___.
Umbrella pattern
What is the rxn. Of L. monocytogenes in hippurate hydrolysis, bile esculin hydrolysis , and in CAMP factor?
Positive
In CAMP reaction, what is used to produce block type hemolysis in L. monocytogenes?
Rhodococcus equi
In CAMP test, what spp. Of bacteria want to differentiate from L. monocytogenes?
Listeria spp.
L. monocytogenes is differentiated from S. agalactiae and enterococci by ___.
Positive catalase test and motility
