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BOC Microbiology > Nonfermentative GNB > Flashcards

Nonfermentative GNB Flashcards

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Biology 101 flashcards
1
Q

Where are nonfermentative GNB found and what percent of all GNB are nonfermentative GNB (NFB)?

A
  • water, soil, food, plants, few are normal flora in humans
  • 20%
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2
Q

List general traits of NFB
oxygen requirement
sporulation
carb metabolism
oxidase
TSI
Grow on what plate(s)

A
  • obligate aerobes
  • don’t form spores
  • don’t ferment carbs
  • most are oxidase pos
  • TSI: K/no change
  • SBA and varied MAC growth
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3
Q

Most important NFB

A

Pseudomonas aeruginosa

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4
Q

Pseudomonas fluorescent group

A
  • P. aeruginosa
  • P. fluoresceins
  • P. putida
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5
Q

All members of Pseudomonas fluorescent group produce ____ pigment

A

fluorescein aka pyoverdin
yellow fluorescent pigment

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6
Q

____ agar enhances fluorescein production

A

Cetrimide

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7
Q

P. aeruginosa disease association

A
  • eye (contact lens) infections
  • ear infections (swimmers ear)
  • cystic fibrosis lower resp tract infection
  • burn wound infections
  • immunocomprosmised pt
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8
Q

How does P. aeruginosa fare against disinfectants?

A
  • resistant to a lot of disinfectants
  • nosocomial infections in hospitals, equipment, whirlpools, and swimming pools
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9
Q

P. aeruginosa biochemical traits
oxidase
motility
lactose
growth temp
grows on MAC?
colony color

A
  • oxidase pos
  • motile
  • lactose neg
  • 42°C
  • yes, grows on MAC
  • bluish green pigment
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10
Q

P. aeruginosa virulence factors

A
  • Pili
  • LPS endotoxin
  • Exotoxin A
  • extracellular alginate slime to inhibit phagocytosis
  • proteolytic enzymes
  • intrinsic antimicrobial resistance
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11
Q

P. aeruginosa colony morphology

A
  • large irregular colonies with grape-like odor and metallic sheen on SBA
  • beta hemolytic with feathery edge on SBA
  • mucoid colonies if isolated from CF pt
  • pyocyanin blue pigment unique
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12
Q

Stenotrophomonas maltophilia disease association

A
  • pneumonia
  • UTIs
  • wound infections
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13
Q

How is S. maltophilia acquired?

A

hospitals

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14
Q

S. maltophilia identifying traits
oxidase
maltose
lysine decarboxylase
antimicrobial resistance

A
  • oxidase neg
  • one of only NFB that is oxidase neg and maltose pos
  • one of 2 NFB that is lysine decarboxylase positive
  • resistant to most Abx
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15
Q

Burkholderia cepacia disease association

A
  • nosocomial infections
  • CF pt (2nd to P. aeruginosa)
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16
Q

B. cepacia enhanced on ____ agar, which inhibits _____

A
  • PC
  • P. aeruginosa
17
Q

B. cepacia colony morphology

A

colorless or yellow on nutrient agar

18
Q

B. cepacia biochemical traits
oxidase
lactose
lysine decarboxylase

A
  • oxidase pos
  • lactose pos
  • lysine decarboxylase pos
19
Q

Burkholderia mallei disease association

A

glanders disease of livestock (horses, mules, donkeys)

20
Q

B. mallei transmission and gov’t status

A
  • animal contact transmits to humans
  • potential bioterrorism agent
21
Q

B. mallei biochemical traits
growth on MAC
oxidase
motility
nitrate reduction
glucose OF

A
  • grows on MAC
  • oxidase variable
  • nonmotile
  • reduces nitrate to nitrite without gas
  • oxidizes glucose
22
Q

Burkholderia pseudomallei disease association

A
  • Melioidosis
  • endemic to southeast Asia and northern Australia
23
Q

B. pseudomallei is found where?

A

soil
infections acquired thru skin or inhalation tho

24
Q

B. pseudomallei biochemical traits
growth on MAC
oxidase
motility
reduction of nitrate
carb OF

A
  • grows on MAC
  • oxidase pos
  • motile
  • reduces nitrate to nitrite with gas
  • oxidizes glucose and lactose
25
Q

Acinetobacter
oxygen requirement
cell morphology
normal environment

A
  • obligate aerobe
  • coccobacillus
  • normal flora of GIT and resp tract
26
Q

Acinetobacter disease association

A
  • nosocomial infections
  • UTIs
27
Q

Acinetobacter most common spp to cause nosocomial infection

A

A. baumaniii
multidrug resistance common

28
Q

Is A. baumanii saccharolytic or asaccharolytic?

A

Saccharolytic
makes acid from glucose

29
Q

Acinetobacter biochemical traits
oxidase
motility
nitrate
catalase

A
  • oxidase neg
  • nonmotile
  • nitrate neg
  • catalase pos

BOC Microbiology (7 decks)

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