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BOC Microbiology > Haemophilus and Simliar Organisms > Flashcards

Haemophilus and Simliar Organisms Flashcards

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Biology 101 flashcards
1
Q

Haemophilus spp normal environment

A

upper respiratory tract flora

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2
Q

List HACEK group

A
  • Haemophilus
  • Actinobacillus actinomycetemcomitans
  • Cardiobacterium hominis
  • Eikenella corrodens
  • Kingella
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3
Q

Haemophilus gram stain morphology

A

Pleomorphic GNCB

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4
Q

Haemophilus growth requirements

A
  • X factor released from Hgb
  • V factor (NAD) heat labile
  • found in CHOC
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5
Q

Haemophilus media growth
SBA
rabbit/horse blood agar
CHOC

A
  • does not grow on SBA bc of NADase inactivating NAD
  • growth on rabbit or horse blood agar
  • growth on CHOC bc it supplies X and V factors
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6
Q

Haemophilus growth conditions

A

35-37°C with 5-10% CO2

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7
Q

Haemophilus CHOC colony morphology

A

smooth/round
flat and opaque
tan

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8
Q

Haemophilus satellitism

A
  • Haemophilus spp can grow around colonies of S. aureus on SBA bc S. aureus release NAD
  • Haemophilus shows up as pinpoint colonies
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9
Q

Haemophilus biochemical traits
oxidase
catalase
motility

A
  • oxidase pos
  • catalase pos
  • nonmotile
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10
Q

H. influenzae number of capsular serotypes and biotypes

A
  • 6 capsular serotypes a-f
  • eight biotypes I-VIII
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11
Q

H. influenzae type b disease association

A
  • meningitis in kids
  • Hib vax reduced disease
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12
Q

H. influenzae isolates should be tested for ______

A

beta-lactamase

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13
Q

H. aegyptius disease assocation

A

pink eye (contagious conjunctivitis)

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14
Q

Carb fermentation difference between H. influenzae and H. aegyptius

A

H. aegyptius is sucrose pos and the other is not

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15
Q

H. influenzae biogroup aegyptius disease assocation

A

conjunctivitis followed by Brazilian purpuric fever

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16
Q

H. influenzae biogroup aegyptius resembles_______ in the following biochemical aspects: indole, urease, ODC

A
  • resembles H. influenzae biotype III
  • indole neg
  • urease pos
  • ODC neg
17
Q

H. ducreyi disease association

A
  • STD genital ulcers
  • chancroids and buboes (swollen lymph nodes)
18
Q

H. ducreyi plating

A

CHOC with vancomycin to inhibit normal flora and contaminants

BOC Microbiology (7 decks)

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